PMID- 29048627
OWN - NLM
STAT- MEDLINE
DCOM- 20180702
LR  - 20220318
IS  - 1791-2431 (Electronic)
IS  - 1021-335X (Linking)
VI  - 38
IP  - 5
DP  - 2017 Nov
TI  - SLC7A11, a component of cysteine/glutamate transporter, is a novel biomarker for 
      the diagnosis and prognosis in laryngeal squamous cell carcinoma.
PG  - 3019-3029
LID - 10.3892/or.2017.5976 [doi]
AB  - Solute carrier family 7, membrane 11 (SLC7A11) or (xCT) is a component of the 
      cysteine-glutamate transporter, which plays a critical role in glutathione 
      homeostasis which is important to protect cells from oxidative stress. SLC7A11 is 
      distributed in various tissues and participates in the occurrence of a number of 
      diseases, particularly in the pathogenesis of malignant tumors, but its role in 
      laryngeal cancer development has not yet been clearly defined. The objective of 
      the present study was to investigate the role of SLC7A11 in laryngeal squamous 
      cell carcinoma (LSCC). We conducted immunohistochemistry and RT-PCR to evaluate 
      the protein and mRNA levels of SLC7A11 in LSCC and in control tissues, 
      respectively. The knockdown experiments were conducted with SLC7A11 short hairpin 
      RNA (shRNA) lentivirus, and the protein and mRNA levels of SLC7A11 were assessed 
      by RT-PCR and western blotting. The functional study of SLC7A11 in vitro was 
      conducted by MTT assay, and the effects on the cell cycle were detected using 
      flow cytometry. Immunohistochemical results revealed that the expression levels 
      of SLC7A11, Ki-67 and p53 in LSCC tissues were higher than those in laryngeal 
      dysplasia tissues. The Spearman rank correlation analysis revealed that the 
      expression of SLC7A11 was positively correlated with the expression of p53 and 
      Ki-67. Cox regression analysis and Kaplan-Meier plots confirmed that the 
      expression levels of SLC7A11 were a prognostic factor for overall survival (OS) 
      rates and postoperative recurrence of LSCC. Moreover, the functional study of 
      SLC7A11 in vitro revealed that knockdown of SLC7A11 using shRNA inhibited cell 
      proliferation by inducing cell cycle arrest at the G1 phase. Immunohistochemical 
      and RT-PCR results and knockdown experiments of SLC7A11 revealed that SLC7A11 was 
      involved in the progression of LSCC, and may provide clinical information for the 
      evaluation of OS rates and postoperative recurrence of LSCC. Collectively, these 
      observations suggest that SLC7A11 may be a vital biomarker for the diagnosis and 
      prognosis in human LSCC, and targeting SLC7A11 appears to be a potentially 
      significant method for LSCC treatment.
FAU - Ma, Zhihong
AU  - Ma Z
AD  - Department of Pathology, Beijing Key Laboratory of Head and Neck Molecular 
      Diagnostic Pathology, Beijing Tongren Hospital, Capital Medical University, 
      Beijing 100730, P.R. China.
FAU - Zhang, Hong
AU  - Zhang H
AD  - Department of Pathology, Beijing Key Laboratory of Head and Neck Molecular 
      Diagnostic Pathology, Beijing Tongren Hospital, Capital Medical University, 
      Beijing 100730, P.R. China.
FAU - Lian, Meng
AU  - Lian M
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren 
      Hospital, Capital Medical University, Beijing 100730, P.R. China.
FAU - Yue, Changli
AU  - Yue C
AD  - Department of Pathology, Beijing Key Laboratory of Head and Neck Molecular 
      Diagnostic Pathology, Beijing Tongren Hospital, Capital Medical University, 
      Beijing 100730, P.R. China.
FAU - Dong, Gehong
AU  - Dong G
AD  - Department of Pathology, Beijing Key Laboratory of Head and Neck Molecular 
      Diagnostic Pathology, Beijing Tongren Hospital, Capital Medical University, 
      Beijing 100730, P.R. China.
FAU - Jin, Yulan
AU  - Jin Y
AD  - Department of Pathology, Beijing Key Laboratory of Head and Neck Molecular 
      Diagnostic Pathology, Beijing Tongren Hospital, Capital Medical University, 
      Beijing 100730, P.R. China.
FAU - Li, Rui
AU  - Li R
AD  - Department of Pathology, Beijing Key Laboratory of Head and Neck Molecular 
      Diagnostic Pathology, Beijing Tongren Hospital, Capital Medical University, 
      Beijing 100730, P.R. China.
FAU - Wan, Hongfei
AU  - Wan H
AD  - Department of Pathology, Beijing Key Laboratory of Head and Neck Molecular 
      Diagnostic Pathology, Beijing Tongren Hospital, Capital Medical University, 
      Beijing 100730, P.R. China.
FAU - Wang, Ru
AU  - Wang R
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren 
      Hospital, Capital Medical University, Beijing 100730, P.R. China.
FAU - Wang, Ying
AU  - Wang Y
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren 
      Hospital, Capital Medical University, Beijing 100730, P.R. China.
FAU - Zhai, Jie
AU  - Zhai J
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren 
      Hospital, Capital Medical University, Beijing 100730, P.R. China.
FAU - Ma, Hongzhi
AU  - Ma H
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren 
      Hospital, Capital Medical University, Beijing 100730, P.R. China.
FAU - Feng, Ling
AU  - Feng L
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren 
      Hospital, Capital Medical University, Beijing 100730, P.R. China.
FAU - Han, Jianfeng
AU  - Han J
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren 
      Hospital, Capital Medical University, Beijing 100730, P.R. China.
FAU - Liu, Shuzhou
AU  - Liu S
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren 
      Hospital, Capital Medical University, Beijing 100730, P.R. China.
FAU - Guo, Youxin
AU  - Guo Y
AD  - Department of Otolaryngology, Chengde Central Hospital, Chengde, Hebei 067000, 
      P.R. China.
FAU - Li, Jianhua
AU  - Li J
AD  - Department of Surgical Oncology, Chengde Central Hospital, Chengde, Hebei 067000, 
      P.R. China.
FAU - Liu, Yu
AU  - Liu Y
AD  - Department of Pathology, School of Basic Medical Sciences, Capital Medical 
      University, Beijing 100730, P.R. China.
FAU - Fang, Jugao
AU  - Fang J
AD  - Department of Pathology, Beijing Key Laboratory of Head and Neck Molecular 
      Diagnostic Pathology, Beijing Tongren Hospital, Capital Medical University, 
      Beijing 100730, P.R. China.
FAU - Liu, Honggang
AU  - Liu H
AD  - Department of Pathology, Beijing Key Laboratory of Head and Neck Molecular 
      Diagnostic Pathology, Beijing Tongren Hospital, Capital Medical University, 
      Beijing 100730, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20170920
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (Amino Acid Transport System X-AG)
RN  - 0 (Amino Acid Transport System y+)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (SLC7A11 protein, human)
RN  - K848JZ4886 (Cysteine)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Amino Acid Transport System X-AG/genetics
MH  - Amino Acid Transport System y+/*genetics
MH  - Apoptosis/genetics
MH  - Biomarkers, Tumor/*genetics
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - Cell Proliferation/genetics
MH  - Cysteine/genetics
MH  - Disease-Free Survival
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Head and Neck Neoplasms/*genetics/pathology
MH  - Humans
MH  - Kaplan-Meier Estimate
MH  - Laryngeal Neoplasms/*genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Recurrence, Local/*genetics/pathology
MH  - Prognosis
MH  - Squamous Cell Carcinoma of Head and Neck
EDAT- 2017/10/20 06:00
MHDA- 2018/07/03 06:00
CRDT- 2017/10/20 06:00
PHST- 2017/01/22 00:00 [received]
PHST- 2017/07/14 00:00 [accepted]
PHST- 2017/10/20 06:00 [pubmed]
PHST- 2018/07/03 06:00 [medline]
PHST- 2017/10/20 06:00 [entrez]
AID - 10.3892/or.2017.5976 [doi]
PST - ppublish
SO  - Oncol Rep. 2017 Nov;38(5):3019-3029. doi: 10.3892/or.2017.5976. Epub 2017 Sep 20.