PMID- 29048659
OWN - NLM
STAT- MEDLINE
DCOM- 20180702
LR  - 20220331
IS  - 1791-2431 (Electronic)
IS  - 1021-335X (Linking)
VI  - 38
IP  - 5
DP  - 2017 Nov
TI  - MicroRNA-520c-3p negatively regulates EMT by targeting IL-8 to suppress the 
      invasion and migration of breast cancer.
PG  - 3144-3152
LID - 10.3892/or.2017.5968 [doi]
AB  - Interleukin-8 (IL-8), which is secreted by cancer cells undergoing 
      epithelial-mesenchymal transition (EMT), can promote EMT in adjacent 
      epithelial-like cells. MicroRNAs (miRNAs/miRs) can affect the expression of 
      target genes via binding to their 3'-untranslated regions (3'-UTRs), which may 
      subsequently affect the biological behaviors of cancer cells. In our previous 
      study, miR-520c-3p was predicted to directly target the 3'-UTR of IL-8. 
      Therefore, the present study was carried out to investigate whether miR-520c-3p 
      can interact with the IL-8 gene and regulate the EMT of breast cancer cells. 
      Web-based prediction algorithms were used to identify miRNAs that potentially 
      target the IL-8 transcript. Luciferase reporter assays were used to confirm the 
      targeting of IL-8 by miR-520c-3p. Reverse transcription-quantitative PCR and 
      western blot analyses were used to examine the levels of IL-8 and EMT-related 
      genes in breast cancer cells. The functional impact of miR-520c-3p on EMT 
      phenotype was evaluated using Transwell and wound-healing assays, and rescue 
      experiments were conducted by overexpressing IL-8 to determine its effect on cell 
      properties. miR-520c-3p was predicted by all three databases, which strongly 
      suggested its interaction with the 3'-UTR of IL-8. The relative Renilla 
      luciferase activity of luciferase reporter construct containing the wild-type 
      3'-UTR of IL-8 was markedly decreased by miR-520c-3p transfection when compared 
      with scrambled miRNA control transfection (P<0.001). In addition, compared with 
      the scrambled miRNA control transfection, the overexpression of miR-520c-3p 
      significantly reduced the expression of IL-8, and resulted in increased 
      E-cadherin and decreased vimentin and fibronectin levels in MCF-7 and T47D cells 
      (all P<0.001). Introduction of miR-520c-3p inhibited the invasion and migration 
      of MCF-7 and T47D cells (all P<0.001). By contrast, the rescue of IL-8 expression 
      led to the recovery of EMT-related protein expression patterns and cell motility 
      and invasion capabilities. In conclusion, aberrant miR-520c-3p expression may 
      lead to reduced IL-8 expression and promote the mesenchymal phenotype in breast 
      cancer cells, thereby increasing invasive growth.
FAU - Tang, Cui-Ping
AU  - Tang CP
AD  - Department of Oncology, The First Affiliated Hospital of Chongqing Medical 
      University, Yuzhong, Chongqing 400016, P.R. China.
FAU - Zhou, Han-Jing
AU  - Zhou HJ
AD  - Department of Oncology, The First Affiliated Hospital of Chongqing Medical 
      University, Yuzhong, Chongqing 400016, P.R. China.
FAU - Qin, Jian
AU  - Qin J
AD  - Department of Oncology, The First Affiliated Hospital of Chongqing Medical 
      University, Yuzhong, Chongqing 400016, P.R. China.
FAU - Luo, Yi
AU  - Luo Y
AD  - Department of Oncology, The First Affiliated Hospital of Chongqing Medical 
      University, Yuzhong, Chongqing 400016, P.R. China.
FAU - Zhang, Tao
AU  - Zhang T
AD  - Department of Oncology, The First Affiliated Hospital of Chongqing Medical 
      University, Yuzhong, Chongqing 400016, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20170919
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (CXCL8 protein, human)
RN  - 0 (Interleukin-8)
RN  - 0 (MIRN520 microRNA, human)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Breast Neoplasms/*genetics/pathology
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - Epithelial-Mesenchymal Transition/genetics
MH  - Female
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Humans
MH  - Interleukin-8/*genetics
MH  - MCF-7 Cells
MH  - MicroRNAs/*genetics
MH  - Neoplasm Invasiveness/*genetics/pathology
MH  - Signal Transduction/genetics
EDAT- 2017/10/20 06:00
MHDA- 2018/07/03 06:00
CRDT- 2017/10/20 06:00
PHST- 2017/03/30 00:00 [received]
PHST- 2017/08/25 00:00 [accepted]
PHST- 2017/10/20 06:00 [pubmed]
PHST- 2018/07/03 06:00 [medline]
PHST- 2017/10/20 06:00 [entrez]
AID - 10.3892/or.2017.5968 [doi]
PST - ppublish
SO  - Oncol Rep. 2017 Nov;38(5):3144-3152. doi: 10.3892/or.2017.5968. Epub 2017 Sep 19.