PMID- 29048816
OWN - NLM
STAT- MEDLINE
DCOM- 20181109
LR  - 20181109
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 35
IP  - 8
DP  - 2017 Aug 8
TI  - [Determination of nicotine and cotinine in urine by hydrophilic interaction 
      chromatography-tandem mass spectrometry].
PG  - 826-831
LID - 10.3724/SP.J.1123.2017.05019 [doi]
AB  - The analytical method of nicotine and cotinine in human urine with hydrophilic 
      interaction chromatography-tandem mass spectrometry (HILIC-MS/MS) was 
      established. After the urine sample containing nicotine-d(4) and cotinine-d(3) 
      isotope internal standards being diluted with water, the filtrate was introduced 
      into ultra performance liquid chromatography-tandem mass spectrometry 
      (UPLC-MS/MS) for analysis. Separation was performed on an ACQUITY UPLC((R)) BEH 
      HILIC column (50 mmx3.0 mm, 1.7 mu m), in which methanol and 0.1% (v/v) ammonia 
      were used as the mobile phases with isocratic elution at 0.2 mL/min of flow rate. 
      Positive ion scan mode was used for mass spectrometry measurement and calibration 
      curves were plotted for quantification determination. A good linearity could be 
      obtained in the range of 1.0-1000 mu g/L for nicotine and cotinine with the linear 
      coefficients of 0.9949 and 0.9958, respectively. The limits of detection of 
      nicotine and cotinine were 0.082 mu g/L and 0.077 mu g/L, and the limits of 
      quantification were 0.27 mu g/L and 0.26 mu g/L, respectively. The recoveries of 
      the spiked urine samples were 90.4%-103.5% and 93.0%-104.6%, and the relative 
      standard deviations (RSDs) were 4.80%-6.21% and 4.22%-7.15% for nicotine and 
      cotinine respectively. The established method was applied to the analyis of 200 
      urine samples. Based on the investigation information of the urine of the smoking 
      people, the nicotine contents were 26.68-854.30 mu g/L, and the cotinine contents 
      were 36.66-1191.18 mu g/L (n=86, M(nicotine)=76.00 mu g/L, M(cotinine)=83.52 mu g/L, 
      M:median); of the nonsmoking people, the nicotine contents were 5.08-69.66 mu g/L, 
      and the cotinine contents were 3.16-28.21 mu g/L (n=114, M(nicotine)=7.53 mu g/L, 
      M(cotinine)=3.79 mu g/L). The method is simple, sensitive and rapid. It is 
      suitable for batch analysis of nicotine and cotinine in urine, and it can meet 
      the requirement of evaluating the human tobacco exposure.
FAU - Li, Mingxin
AU  - Li M
AD  - Department of Laboratory Techonology and Science of Public Health, West China 
      School of Public Health, Sichuan University, Chengdu 610041, China.
FAU - Wang, Qian
AU  - Wang Q
AD  - Department of Laboratory Techonology and Science of Public Health, West China 
      School of Public Health, Sichuan University, Chengdu 610041, China.
FAU - Zhu, Jing
AU  - Zhu J
AD  - Department of Laboratory Techonology and Science of Public Health, West China 
      School of Public Health, Sichuan University, Chengdu 610041, China.
FAU - Ren, Lin
AU  - Ren L
AD  - Center for Disease Control and Prevention of Sichuan Province, Chengdu 610041, 
      China.
FAU - Yong, Li
AU  - Yong L
AD  - Center for Disease Control and Prevention of Sichuan Province, Chengdu 610041, 
      China.
FAU - Zou, Xiaoli
AU  - Zou X
AD  - Department of Laboratory Techonology and Science of Public Health, West China 
      School of Public Health, Sichuan University, Chengdu 610041, China. 
      zouxl_1113@163.com.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 6M3C89ZY6R (Nicotine)
RN  - K5161X06LL (Cotinine)
SB  - IM
MH  - Chromatography, High Pressure Liquid
MH  - Chromatography, Liquid
MH  - Cotinine/*urine
MH  - Humans
MH  - Hydrophobic and Hydrophilic Interactions
MH  - Nicotine/*urine
MH  - *Tandem Mass Spectrometry
OTO - NOTNLM
OT  - cotinine
OT  - hydrophilic interaction chromatography-tandem mass spectrometry (HILIC-MS/MS)
OT  - isotope dilution
OT  - nicotine
OT  - urine
EDAT- 2017/10/20 06:00
MHDA- 2018/11/10 06:00
CRDT- 2017/10/20 06:00
PHST- 2017/10/20 06:00 [entrez]
PHST- 2017/10/20 06:00 [pubmed]
PHST- 2018/11/10 06:00 [medline]
AID - 10.3724/SP.J.1123.2017.05019 [doi]
PST - ppublish
SO  - Se Pu. 2017 Aug 8;35(8):826-831. doi: 10.3724/SP.J.1123.2017.05019.