PMID- 29048834
OWN - NLM
STAT- MEDLINE
DCOM- 20181109
LR  - 20181109
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 35
IP  - 7
DP  - 2017 Jul 8
TI  - [Preparation and performance characterization of gold nanoparticles modified 
      chiral capillary electrochromatography stationary phase].
PG  - 712-718
LID - 10.3724/SP.J.1123.2017.03019 [doi]
AB  - Gold nanoparticles (GNPs, 15 nm) were prepared and introduced to amino groups 
      derived silica monolithic column. Bovine serum albumin (BSA) was immobilized via 
      covalent modification method onto the carboxylic functionalized GNPs to afford 
      chiral stationary phase (CSP) for enantioseparation. GNPs were well dispersed and 
      successfully incorporated onto the columns with the contents as high as 17.18% by 
      characterization method such as transmission electron microscopy (TEM), 
      ultraviolet (UV)-visible absorption spectra and scanning electron microscopy 
      (SEM). The preparation conditions of the BSA modified CSP were optimized and 10% 
      (v/v) 3-aminopropyltriethoxysilane (APTES) and 15 g/L BSA were selected as 
      appropriate reaction conditions. The enantioseparation performance of the BSA 
      modified CSP has been investigated by capillary electrochromatography (CEC). 
      Enantiomers of tryptophan, ephedrine and atenolol were resolved, and the baseline 
      separation of tryptophan was achieved. Meanwhile, the influences of pH value, 
      buffer concentrations and applied voltages used on the chiral separation were 
      studied, and the optimal separation conditions were 10 mmol/L phosphate buffer at 
      pH 7.4 and 15 kV applied voltages. In comparison with the BSA modified CSP 
      prepared by physical adsorption, the CSP prepared by covalent modification method 
      had better separation results, and the analytes could be separated directly 
      without pre-column derivatization. In addition, the prepared BSA modified CSP 
      exhibited good run to run repeatability with relative standard deviations (RSDs) 
      of the migration times and selectivity factors not more than 2.3% and 0.96%, 
      respectively. This work offers a good thinking for modification with other 
      proteins or other types of chiral selectors.
FAU - Xiong, Lele
AU  - Xiong L
AD  - College of Science, China Pharmaceutical University, Nanjing 210009, China.
FAU - Li, Ruijun
AU  - Li R
AD  - College of Science, China Pharmaceutical University, Nanjing 210009, China.
FAU - Ji, Yibing
AU  - Ji Y
AD  - College of Science, China Pharmaceutical University, Nanjing 210009, China. 
      jiyibing@msn.com.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 7440-57-5 (Gold)
RN  - 7631-86-9 (Silicon Dioxide)
RN  - 8DUH1N11BX (Tryptophan)
SB  - IM
MH  - Adsorption
MH  - *Capillary Electrochromatography
MH  - *Gold
MH  - *Metal Nanoparticles
MH  - Microscopy, Electron, Scanning
MH  - Microscopy, Electron, Transmission
MH  - Serum Albumin, Bovine
MH  - Silicon Dioxide
MH  - Stereoisomerism
MH  - Tryptophan
OTO - NOTNLM
OT  - capillary electrochromatography (CEC)
OT  - chiral stationary phase (CSP)
OT  - gold nanoparticles (GNPs)
OT  - silica monolithic column (CSM)
EDAT- 2017/10/20 06:00
MHDA- 2018/11/10 06:00
CRDT- 2017/10/20 06:00
PHST- 2017/10/20 06:00 [entrez]
PHST- 2017/10/20 06:00 [pubmed]
PHST- 2018/11/10 06:00 [medline]
AID - 10.3724/SP.J.1123.2017.03019 [doi]
PST - ppublish
SO  - Se Pu. 2017 Jul 8;35(7):712-718. doi: 10.3724/SP.J.1123.2017.03019.