PMID- 29048852
OWN - NLM
STAT- MEDLINE
DCOM- 20190205
LR  - 20190215
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 35
IP  - 9
DP  - 2017 Sep 8
TI  - [Rapid determination of 15 nutrients in rice by ultra high performance liquid 
      chromatography-linear ion trap/orbitrap high resolution mass spectrometry].
PG  - 949-956
LID - 10.3724/SP.J.1123.2017.05001 [doi]
AB  - A rapid method was developed for the simultaneous determination of 15 nutrients 
      containing eight vitamin E, six gamma-oryzanols and beta-carotene in rice by ultra high 
      performance liquid chromatography-linear ion trap/orbitrap high resolution mass 
      spectrometry (UHPLC-LTQ/Orbitrap HRMS). The analytes were extracted by methanol 
      containing 0.05% (v/v) 2,6-di-tert-butyl-4-methylphenol (BHT) under the 
      ultrasonic condition, then separated by a Poroshell 120 PFP column (150 mmx3.0 
      mm, 2.7 mum) with a gradient elution program using 0.1% (v/v) formic acid aqueous 
      solution and methanol containing 0.1% (v/v) formic acid as mobile phases. The 
      detection was performed using an LTQ/Orbitrap HRMS detector with full scan in 
      positive ion mode. Fifteen nutrients were simultaneously separated within 13 min. 
      Furthermore, matrix effects of the white and brown rices were investigated. The 
      correlation coefficients (r) were >/= 0.9950 in the linear ranges of the 15 
      nutrients. The limits of detection (LODs, S/N=3) and limits of quantification 
      (LOQs, S/N=10) of the 15 nutrients were 0.2-1.8 mug/L and 0.7-6.1 mug/L, 
      respectively. At the three spiked levels, the recoveries were 73.2%-101.5% with 
      the relative standard deviations (RSDs) ranging from 1.1% to 5.0% (n=3). The 
      method is accurate, efficient and reliable. It is suitable for the simultaneous 
      determination of various nutrients in rice.
FAU - Zhu, Li
AU  - Zhu L
AD  - Shenzhen Academy of Metrology and Quality Inspection, Shenzhen 518109, China. 
      foodtestzl@126.com.
FAU - Tan, Wei
AU  - Tan W
AD  - Department of Food and Chemical Engineering, Lushan College of Guangxi University 
      of Science and Technology, Liuzhou 545616, China.
FAU - Peng, Zumao
AU  - Peng Z
AD  - Shenzhen Academy of Metrology and Quality Inspection, Shenzhen 518109, China.
FAU - Deng, Mengya
AU  - Deng M
AD  - Shenzhen Academy of Metrology and Quality Inspection, Shenzhen 518109, China.
FAU - Zhang, Xieguang
AU  - Zhang X
AD  - Shenzhen Academy of Metrology and Quality Inspection, Shenzhen 518109, China.
FAU - Yang, Guowu
AU  - Yang G
AD  - Shenzhen Academy of Metrology and Quality Inspection, Shenzhen 518109, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Nutrients)
SB  - IM
MH  - *Chromatography, High Pressure Liquid
MH  - Limit of Detection
MH  - *Mass Spectrometry
MH  - Nutrients/*analysis
MH  - Oryza/*chemistry
OTO - NOTNLM
OT  - linear ion trap-orbitrap high resolution mass spectrometer (LTQ/Orbitrap HRMS)
OT  - nutrients
OT  - rice
OT  - ultra high performance liquid chromatography (UHPLC)
OT  - vitamin E
OT  - beta-carotene
OT  - gamma-oryzanol
EDAT- 2017/10/20 06:00
MHDA- 2019/02/06 06:00
CRDT- 2017/10/20 06:00
PHST- 2017/10/20 06:00 [entrez]
PHST- 2017/10/20 06:00 [pubmed]
PHST- 2019/02/06 06:00 [medline]
AID - 10.3724/SP.J.1123.2017.05001 [doi]
PST - ppublish
SO  - Se Pu. 2017 Sep 8;35(9):949-956. doi: 10.3724/SP.J.1123.2017.05001.