PMID- 29048858
OWN - NLM
STAT- MEDLINE
DCOM- 20190205
LR  - 20190215
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 35
IP  - 9
DP  - 2017 Sep 8
TI  - [Simultaneous determination of diminazene aceturate and isometamidium chloride 
      residues in cattle tissues by high performance liquid chromatography with 
      solid-phase extraction].
PG  - 995-1002
LID - 10.3724/SP.J.1123.2017.04033 [doi]
AB  - A method for the simultaneous determination of trypanocidal diminazene aceturate 
      (DIM) and isometamidium chloride (ISM) that containing benzamidine groups in 
      cattle tissues was developed by high performance liquid chromatography (HPLC) 
      with solid-phase extraction (SPE). The tissue samples were extracted with 
      different proportions of water-acetonitrile, then were cleaned up by Oasis WCX 
      cartridges. DIM and ISM were separated by HPLC with a Spherisorb CN column (250 
      mmx4.6 mm, 5 mum). Acetonitrile-0.05 mol/L ammonium formate solution (pH 2.4) was 
      used as mobile phases with gradient elution. The detection wavelength of UV was 
      set at 380 nm. The limits of detection (LODs) and the limits of quantification 
      (LOQs) of DIM and ISM in cattle tissues were 0.01 mg/kg and 0.025 mg/kg, 
      respectively. The correlation coefficients (r) of DIM and ISM in cattle tissues 
      were not less than 0.9993. The average recoveries of DIM and ISM at three spiked 
      levels were 82.2%-97.6% with the intra-day relative standard derivations (RSDs) 
      of 0.3%-5.2% (n=5) and inter-day RSDs of 1.3%-5.2% (n=15). The method was 
      successfully applied to the analysis of DIM and ISM in cattle tissues. The method 
      is rapid, sensitive and repeatable for the determination of diminazene aceturate 
      and isometamidium chloride in cattle tissues.
FAU - Zhou, Yuhui
AU  - Zhou Y
AD  - College of Veterinary Medicine, South China Agricultural University, National 
      Reference Laboratory of Veterinary Drug Residues, Guangzhou 510642, China.
FAU - Zeng, Zhenling
AU  - Zeng Z
AD  - College of Veterinary Medicine, South China Agricultural University, National 
      Reference Laboratory of Veterinary Drug Residues, Guangzhou 510642, China.
FAU - Yu, Jingxian
AU  - Yu J
AD  - College of Veterinary Medicine, South China Agricultural University, National 
      Reference Laboratory of Veterinary Drug Residues, Guangzhou 510642, China.
FAU - Liu, Rong
AU  - Liu R
AD  - College of Veterinary Medicine, South China Agricultural University, National 
      Reference Laboratory of Veterinary Drug Residues, Guangzhou 510642, China.
FAU - Pan, Lihua
AU  - Pan L
AD  - College of Veterinary Medicine, South China Agricultural University, National 
      Reference Laboratory of Veterinary Drug Residues, Guangzhou 510642, China.
FAU - Zhong, Qiuyu
AU  - Zhong Q
AD  - College of Veterinary Medicine, South China Agricultural University, National 
      Reference Laboratory of Veterinary Drug Residues, Guangzhou 510642, China.
FAU - Chen, Hong
AU  - Chen H
AD  - College of Veterinary Medicine, South China Agricultural University, National 
      Reference Laboratory of Veterinary Drug Residues, Guangzhou 510642, China. 
      shuyi@scau.edu.cn.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Phenanthridines)
RN  - 7NH28I651F (isometamidium chloride)
RN  - JI8SAD85NO (diminazene aceturate)
RN  - Y5G36EEA5Z (Diminazene)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Chromatography, High Pressure Liquid
MH  - Diminazene/*analogs & derivatives/analysis
MH  - Drug Residues/*analysis
MH  - Food Contamination/*analysis
MH  - Phenanthridines/*analysis
MH  - Red Meat/*analysis
MH  - Solid Phase Extraction
OTO - NOTNLM
OT  - cattle tissues
OT  - diminazene aceturate (DIM)
OT  - high performance liquid chromatography (HPLC)
OT  - isometamidium chloride (ISM)
OT  - multi-residue determination
OT  - solid-phase extraction (SPE)
EDAT- 2017/10/20 06:00
MHDA- 2019/02/06 06:00
CRDT- 2017/10/20 06:00
PHST- 2017/10/20 06:00 [entrez]
PHST- 2017/10/20 06:00 [pubmed]
PHST- 2019/02/06 06:00 [medline]
AID - 10.3724/SP.J.1123.2017.04033 [doi]
PST - ppublish
SO  - Se Pu. 2017 Sep 8;35(9):995-1002. doi: 10.3724/SP.J.1123.2017.04033.