PMID- 29051198
OWN - NLM
STAT- MEDLINE
DCOM- 20180806
LR  - 20240327
IS  - 1532-2548 (Electronic)
IS  - 0032-0889 (Print)
IS  - 0032-0889 (Linking)
VI  - 175
IP  - 4
DP  - 2017 Dec
TI  - Abscisic Acid-Induced Reactive Oxygen Species Are Modulated by Flavonols to 
      Control Stomata Aperture.
PG  - 1807-1825
LID - 10.1104/pp.17.01010 [doi]
AB  - Abscisic acid (ABA) increases reactive oxygen species (ROS) in guard cells to 
      close Arabidopsis (Arabidopsis thaliana) stomata. In tomato (Solanum 
      lycopersicum), we find that ABA-increased ROS is followed by stomatal closure and 
      that both responses are blocked by inhibitors of ROS-producing respiratory burst 
      oxidase enzymes. ABA-induced ROS sensor fluorescence accumulates in the nucleus, 
      chloroplasts, and endomembranes. The accumulation of flavonol antioxidants in 
      guard cells, but not surrounding pavement cells, was visualized by confocal 
      microscopy using a flavonol-specific fluorescent dye. Decreased flavonols in 
      guard cells in the anthocyanin reduced (are) mutant and elevated levels in the 
      anthocyanin without (aw) mutant were quantified by confocal microscopy and in 
      leaf extracts by mass spectrometry. Consistent with flavonols acting as 
      antioxidants, higher levels of ROS were detected in guard cells of the tomato are 
      mutant and lower levels were detected in aw both at homeostasis and after 
      treatment with ABA. These results demonstrate the inverse relationship between 
      flavonols and ROS. Guard cells of are show greater ABA-induced closure than the 
      wild type, reduced light-dependent guard cell opening, and reduced water loss, 
      with aw having opposite responses. Ethylene treatment of wild-type tomato plants 
      increased flavonol accumulation in guard cells; however, no flavonol increases 
      were observed in Neverripe (Nr), an ethylene receptor mutant. Consistent with 
      lower levels of ROS due to elevated flavonols, ethylene treatments decreased 
      ABA-induced stomatal closure in the wild type, but not Nr, with ethylene 
      responses attenuated in the are mutant. Together, these results are consistent 
      with flavonols dampening the ABA-dependent ROS burst that drives stomatal closure 
      and facilitating stomatal opening to modulate leaf gas exchange.
CI  - (c) 2017 American Society of Plant Biologists. All Rights Reserved.
FAU - Watkins, Justin M
AU  - Watkins JM
AUID- ORCID: 0000-0003-4127-1950
AD  - Wake Forest University, Department of Biology and Center for Molecular Signaling, 
      Winston-Salem, North Carolina 27109.
FAU - Chapman, Jordan M
AU  - Chapman JM
AUID- ORCID: 0000-0001-6755-9341
AD  - Wake Forest University, Department of Biology and Center for Molecular Signaling, 
      Winston-Salem, North Carolina 27109.
FAU - Muday, Gloria K
AU  - Muday GK
AUID- ORCID: 0000-0002-0377-4517
AD  - Wake Forest University, Department of Biology and Center for Molecular Signaling, 
      Winston-Salem, North Carolina 27109 muday@wfu.edu.
LA  - eng
PT  - Journal Article
DEP - 20171019
PL  - United States
TA  - Plant Physiol
JT  - Plant physiology
JID - 0401224
RN  - 0 (Ethylenes)
RN  - 0 (Flavonols)
RN  - 0 (Plant Proteins)
RN  - 0 (Reactive Oxygen Species)
RN  - 72S9A8J5GW (Abscisic Acid)
RN  - 91GW059KN7 (ethylene)
SB  - IM
MH  - Abscisic Acid
MH  - Ethylenes/pharmacology
MH  - Flavonols/chemistry/*metabolism
MH  - Gene Expression Regulation, Plant/physiology
MH  - Homeostasis
MH  - Light
MH  - Solanum lycopersicum/genetics/*physiology
MH  - Molecular Structure
MH  - Mutation
MH  - Plant Proteins/genetics/metabolism
MH  - Plant Stomata/*physiology
MH  - Plant Transpiration/physiology
MH  - Reactive Oxygen Species/*metabolism
MH  - Signal Transduction
PMC - PMC5717730
EDAT- 2017/10/21 06:00
MHDA- 2018/08/07 06:00
PMCR- 2017/10/19
CRDT- 2017/10/21 06:00
PHST- 2017/07/24 00:00 [received]
PHST- 2017/10/17 00:00 [accepted]
PHST- 2017/10/21 06:00 [pubmed]
PHST- 2018/08/07 06:00 [medline]
PHST- 2017/10/21 06:00 [entrez]
PHST- 2017/10/19 00:00 [pmc-release]
AID - pp.17.01010 [pii]
AID - PP2017RA01010DR1 [pii]
AID - 10.1104/pp.17.01010 [doi]
PST - ppublish
SO  - Plant Physiol. 2017 Dec;175(4):1807-1825. doi: 10.1104/pp.17.01010. Epub 2017 Oct 
      19.