PMID- 29052022
OWN - NLM
STAT- MEDLINE
DCOM- 20180813
LR  - 20231213
IS  - 1618-0860 (Electronic)
IS  - 0918-9440 (Linking)
VI  - 131
IP  - 2
DP  - 2018 Mar
TI  - UDP-arabinopyranose mutase gene expressions are required for the biosynthesis of 
      the arabinose side chain of both pectin and arabinoxyloglucan, and normal leaf 
      expansion in Nicotiana tabacum.
PG  - 307-317
LID - 10.1007/s10265-017-0985-6 [doi]
AB  - Plant cell walls are composed of polysaccharides such as cellulose, 
      hemicelluloses, and pectins, whose location and function differ depending on 
      plant type. Arabinose is a constituent of many different cell wall components, 
      including pectic rhamnogalacturonan I (RG-I) and II (RG-II), 
      glucuronoarabinoxylans (GAX), and arabinoxyloglucan (AXG). Arabinose is found 
      predominantly in the furanose rather than in the thermodynamically more stable 
      pyranose form. The UDP-arabinopyranose mutases (UAMs) have been demonstrated to 
      convert UDP-arabinopyranose (UDP-Arap) to UDP-arabinofuranose (UDP-Araf) in rice 
      (Oryza sativa L.). The UAMs have been implicated in polysaccharide biosynthesis 
      and developmental processes. Arabinose residues could be a component of many 
      polysaccharides, including branched (1-->5)-alpha-arabinans, arabinogalactans in pectic 
      polysaccharides, and arabinoxyloglucans, which are abundant in the cell walls of 
      solanaceous plants. Therefore, to elucidate the role of UAMs and arabinan side 
      chains, we analyzed the UAM RNA interference transformants in tobacco (Nicotiana 
      tabacum L.). The tobacco UAM gene family consists of four members. We generated 
      RNAi transformants (NtUAM-KD) to down-regulate all four of the UAM members. The 
      NtUAM-KD showed abnormal leaf development in the form of a callus-like structure 
      and many holes in the leaf epidermis. A clear reduction in the pectic arabinan 
      content was observed in the tissue of the NtUAM-KD leaf. The arabinose/xylose 
      ratio in the xyloglucan-rich cell wall fraction was drastically reduced in 
      NtUAM-KD. These results suggest that UAMs are required for Ara side chain 
      biosynthesis in both RG-I and AXG in Solanaceae plants, and that 
      arabinan-mediated cell wall networks might be important for normal leaf 
      expansion.
FAU - Honta, Hideyuki
AU  - Honta H
AD  - Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, 
      Ibaraki, 305-8572, Japan.
FAU - Inamura, Takuya
AU  - Inamura T
AD  - Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, 
      Ibaraki, 305-8572, Japan.
FAU - Konishi, Teruko
AU  - Konishi T
AD  - Department of Bioscience and Biotechnology, Faculty of Agriculture, University of 
      the Ryukyus, Nishihara, Okinawa, 903-0213, Japan.
FAU - Satoh, Shinobu
AU  - Satoh S
AD  - Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, 
      Ibaraki, 305-8572, Japan.
FAU - Iwai, Hiroaki
AU  - Iwai H
AD  - Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, 
      Ibaraki, 305-8572, Japan. iwai.hiroaki.gb@u.tsukuba.ac.jp.
LA  - eng
GR  - 24114006/Grant-in-Aid for Scientific Research on Innovative Areas/
GR  - 18075004/Grant-in-Aid for Scientific Research on Priority Areas/
PT  - Journal Article
DEP - 20171019
PL  - Japan
TA  - J Plant Res
JT  - Journal of plant research
JID - 9887853
RN  - 0 (Glucans)
RN  - 0 (Polysaccharides)
RN  - 0 (Uridine Diphosphate Sugars)
RN  - 11078-27-6 (araban)
RN  - 14697-41-7 (uridine diphosphate arabinose)
RN  - 89NA02M4RX (Pectins)
RN  - B40ROO395Z (Arabinose)
RN  - EC 5.4.- (Intramolecular Transferases)
SB  - IM
MH  - Arabinose/metabolism
MH  - *Gene Expression
MH  - Glucans
MH  - Intramolecular Transferases/*genetics/metabolism
MH  - Pectins/metabolism
MH  - Plant Leaves/*growth & development/metabolism
MH  - Polysaccharides/metabolism
MH  - Nicotiana/*genetics/growth & development/metabolism
MH  - Uridine Diphosphate Sugars/metabolism
OTO - NOTNLM
OT  - Arabinoxyloglucan
OT  - Nicotiana tabacum
OT  - Rhamnogalacturonan-I
OT  - UDP-arabinopyranose mutase
EDAT- 2017/10/21 06:00
MHDA- 2018/08/14 06:00
CRDT- 2017/10/21 06:00
PHST- 2017/05/15 00:00 [received]
PHST- 2017/09/04 00:00 [accepted]
PHST- 2017/10/21 06:00 [pubmed]
PHST- 2018/08/14 06:00 [medline]
PHST- 2017/10/21 06:00 [entrez]
AID - 10.1007/s10265-017-0985-6 [pii]
AID - 10.1007/s10265-017-0985-6 [doi]
PST - ppublish
SO  - J Plant Res. 2018 Mar;131(2):307-317. doi: 10.1007/s10265-017-0985-6. Epub 2017 
      Oct 19.