PMID- 29052333
OWN - NLM
STAT- MEDLINE
DCOM- 20180716
LR  - 20210109
IS  - 1615-9861 (Electronic)
IS  - 1615-9853 (Print)
IS  - 1615-9853 (Linking)
VI  - 17
IP  - 23-24
DP  - 2017 Dec
TI  - Label-Free Quantitative Proteomic Analysis of Differentially Expressed Membrane 
      Proteins of Pulmonary Alveolar Macrophages Infected with Highly Pathogenic 
      Porcine Reproductive and Respiratory Syndrome Virus and Its Attenuated Strain.
LID - 10.1002/pmic.201700101 [doi]
LID - 1700101
AB  - Significant differences exist between the highly pathogenic (HP) porcine 
      reproductive and respiratory syndrome virus (PRRSV) and its attenuated pathogenic 
      (AP) strain in the ability to infect host cells. The mechanisms by which 
      different virulent strains invade host cells remain relatively unknown. In this 
      study, pulmonary alveolar macrophages (PAMs) are infected with HP-PRRSV (HuN4) 
      and AP-PRRSV (HuN4-F112) for 24 h, then harvested and subjected to label-free 
      quantitative MS. A total of 2849 proteins are identified, including 95 that are 
      differentially expressed. Among them, 26 proteins are located on the membrane. 
      The most differentially expressed proteins are involved in response to stimulus, 
      metabolic process, and immune system process, which mainly have the function of 
      binding and catalytic activity. Cluster of differentiation CD163, vimentin (VIM), 
      and nmII as well as detected proteins are assessed together by string analysis, 
      which elucidated a potentially different infection mechanism. According to the 
      function annotations, PRRSV with different virulence may mainly differ in 
      immunology, inflammation, immune evasion as well as cell apoptosis. This is the 
      first attempt to explore the differential characteristics between HP-PRRSV and 
      its attenuated PRRSV infected PAMs focusing on membrane proteins which will be of 
      great help to further understand the different infective mechanisms of HP-PRRSV 
      and AP-PRRSV.
CI  - (c) 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Qu, Zehui
AU  - Qu Z
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai, P. R. China.
FAU - Gao, Fei
AU  - Gao F
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai, P. R. China.
AD  - Jiangsu Co-innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou, P. R. China.
FAU - Li, Liwei
AU  - Li L
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai, P. R. China.
FAU - Zhang, Yujiao
AU  - Zhang Y
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai, P. R. China.
FAU - Jiang, Yifeng
AU  - Jiang Y
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai, P. R. China.
AD  - Jiangsu Co-innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou, P. R. China.
FAU - Yu, Lingxue
AU  - Yu L
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai, P. R. China.
AD  - Jiangsu Co-innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou, P. R. China.
FAU - Zhou, Yanjun
AU  - Zhou Y
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai, P. R. China.
AD  - Jiangsu Co-innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou, P. R. China.
FAU - Zheng, Hao
AU  - Zheng H
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai, P. R. China.
AD  - Jiangsu Co-innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou, P. R. China.
FAU - Tong, Wu
AU  - Tong W
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai, P. R. China.
AD  - Jiangsu Co-innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou, P. R. China.
FAU - Li, Guoxin
AU  - Li G
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai, P. R. China.
AD  - Jiangsu Co-innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou, P. R. China.
FAU - Tong, Guangzhi
AU  - Tong G
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai, P. R. China.
AD  - Jiangsu Co-innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou, P. R. China.
LA  - eng
PT  - Journal Article
DEP - 20171124
PL  - Germany
TA  - Proteomics
JT  - Proteomics
JID - 101092707
RN  - 0 (Membrane Proteins)
RN  - 0 (Proteome)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Host-Pathogen Interactions
MH  - Macrophages, Alveolar/*metabolism/virology
MH  - Membrane Proteins/*analysis
MH  - Porcine Reproductive and Respiratory Syndrome/*metabolism/virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/classification/*pathogenicity/physiology
MH  - Proteome/*analysis
MH  - Proteomics/*methods
MH  - Pulmonary Alveoli/*metabolism/virology
MH  - Swine
MH  - Virulence
PMC - PMC6084361
OTO - NOTNLM
OT  - attenuated
OT  - highly pathogenic
OT  - infection
OT  - label-free quantitative proteomics
OT  - membrane proteins
COIS- The authors declare that they have no conflicts of interest associated with this 
      report.
EDAT- 2017/10/21 06:00
MHDA- 2018/07/17 06:00
PMCR- 2020/04/20
CRDT- 2017/10/21 06:00
PHST- 2017/03/20 00:00 [received]
PHST- 2017/09/19 00:00 [revised]
PHST- 2017/10/21 06:00 [pubmed]
PHST- 2018/07/17 06:00 [medline]
PHST- 2017/10/21 06:00 [entrez]
PHST- 2020/04/20 00:00 [pmc-release]
AID - PMIC12759 [pii]
AID - 10.1002/pmic.201700101 [doi]
PST - ppublish
SO  - Proteomics. 2017 Dec;17(23-24):1700101. doi: 10.1002/pmic.201700101. Epub 2017 
      Nov 24.