PMID- 29053210
OWN - NLM
STAT- MEDLINE
DCOM- 20190306
LR  - 20220318
IS  - 1934-6638 (Electronic)
IS  - 1934-662X (Linking)
VI  - 126
IP  - 1
DP  - 2018 Jan
TI  - A combination of MTAP and BAP1 immunohistochemistry in pleural effusion cytology 
      for the diagnosis of mesothelioma.
PG  - 54-63
LID - 10.1002/cncy.21928 [doi]
AB  - BACKGROUND: Homozygous deletion of 9p21 detected by fluorescence in situ 
      hybridization (FISH) and loss of BRCA1-associated protein 1 (BAP1) expression 
      detected by immunohistochemistry (IHC) are useful for the differentiation between 
      malignant pleural mesothelioma (MPM) and reactive mesothelial hyperplasia. The 
      authors previously described that IHC expression of the protein product of the 
      methylthioadenosine phosphorylase (MTAP) gene, which is localized in the 9p21 
      chromosomal region, was correlated with the deletion status of 9p21 FISH in MPM 
      tissues. In the current study, the authors investigated whether a combination of 
      MTAP and BAP1 IHC could distinguish MPM from reactive mesothelial cells (RMC) in 
      cell blocks obtained from pleural effusions. METHODS: The authors examined IHC 
      expression of MTAP and BAP1 in cell blocks obtained from pleural effusions of 45 
      cases of MPM and 21 cases of reactive mesothelial hyperplasia. Furthermore, IHC 
      expression of MTAP was compared with the deletion status of 9p21 FISH. RESULTS: 
      MTAP and BAP1 IHC differentiated MPM from RMC with 100% specificity for both and 
      sensitivities of 42.2% and 60.0%, respectively. The combination of MTAP and BAP1 
      IHC yielded a sensitivity of 77.8%, which was higher than that of BAP1 IHC alone 
      or 9p21 FISH alone (62.2%). Moreover, a high degree of concordance was observed 
      between the results of MTAP IHC and 9p21 FISH in cell blocks. CONCLUSIONS: A 
      combination of MTAP and BAP1 IHC in cell blocks from pleural effusions appears to 
      be a reliable and useful method for differentiating MPM cells from RMC and can be 
      used in the routine diagnosis of MPM. Cancer Cytopathol 2018;126:54-63. (c) 2017 
      American Cancer Society.
CI  - (c) 2017 American Cancer Society.
FAU - Kinoshita, Yoshiaki
AU  - Kinoshita Y
AUID- ORCID: 0000-0002-8872-8957
AD  - Department of Pathology, Fukuoka University Hospital and School of Medicine, 
      Fukuoka, Japan.
AD  - Department of Respiratory Medicine, Fukuoka University Hospital and School of 
      Medicine, Fukuoka, Japan.
FAU - Hida, Tomoyuki
AU  - Hida T
AD  - Department of Anatomic Pathology, Graduate School of Medical Sciences, Kyushu 
      University, Fukuoka, Japan.
FAU - Hamasaki, Makoto
AU  - Hamasaki M
AD  - Department of Pathology, Fukuoka University Hospital and School of Medicine, 
      Fukuoka, Japan.
FAU - Matsumoto, Shinji
AU  - Matsumoto S
AD  - Department of Pathology, Fukuoka University Hospital and School of Medicine, 
      Fukuoka, Japan.
FAU - Sato, Ayuko
AU  - Sato A
AD  - Department of Pathology, Hyogo College of Medicine, Nishinomiya, Japan.
FAU - Tsujimura, Tohru
AU  - Tsujimura T
AD  - Department of Pathology, Hyogo College of Medicine, Nishinomiya, Japan.
FAU - Kawahara, Kunimitsu
AU  - Kawahara K
AD  - Department of Pathology, Osaka Prefectural Medical Center for Respiratory and 
      Allergic Disease, Habikino, Japan.
FAU - Hiroshima, Kenzo
AU  - Hiroshima K
AD  - Department of Pathology, Tokyo Women's Medical University Yachiyo Medical Center, 
      Yachiyo, Japan.
FAU - Oda, Yoshinao
AU  - Oda Y
AD  - Department of Anatomic Pathology, Graduate School of Medical Sciences, Kyushu 
      University, Fukuoka, Japan.
FAU - Nabeshima, Kazuki
AU  - Nabeshima K
AD  - Department of Pathology, Fukuoka University Hospital and School of Medicine, 
      Fukuoka, Japan.
LA  - eng
PT  - Journal Article
DEP - 20171020
PL  - United States
TA  - Cancer Cytopathol
JT  - Cancer cytopathology
JID - 101499453
RN  - 0 (BAP1 protein, human)
RN  - 0 (Tumor Suppressor Proteins)
RN  - EC 2.4.2.1 (Purine-Nucleoside Phosphorylase)
RN  - EC 2.4.2.28 (5'-methylthioadenosine phosphorylase)
RN  - EC 3.4.19.12 (Ubiquitin Thiolesterase)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Lung Neoplasms/*diagnosis/pathology
MH  - Male
MH  - Mesothelioma/*diagnosis/pathology
MH  - Mesothelioma, Malignant
MH  - Middle Aged
MH  - Pleural Effusion/*pathology
MH  - Pleural Neoplasms/*diagnosis/pathology
MH  - Purine-Nucleoside Phosphorylase/*analysis
MH  - Tumor Suppressor Proteins/*analysis
MH  - Ubiquitin Thiolesterase/*analysis
OTO - NOTNLM
OT  - 9p21 fluorescence in situ hybridization
OT  - cell block
OT  - malignant pleural mesothelioma
OT  - pleural effusion
OT  - reactive mesothelial hyperplasia
EDAT- 2017/10/21 06:00
MHDA- 2019/03/07 06:00
CRDT- 2017/10/21 06:00
PHST- 2017/07/19 00:00 [received]
PHST- 2017/09/07 00:00 [revised]
PHST- 2017/09/08 00:00 [accepted]
PHST- 2017/10/21 06:00 [pubmed]
PHST- 2019/03/07 06:00 [medline]
PHST- 2017/10/21 06:00 [entrez]
AID - 10.1002/cncy.21928 [doi]
PST - ppublish
SO  - Cancer Cytopathol. 2018 Jan;126(1):54-63. doi: 10.1002/cncy.21928. Epub 2017 Oct 
      20.