PMID- 29053429 OWN - NLM STAT- MEDLINE DCOM- 20180928 LR - 20181001 IS - 1943-3700 (Electronic) IS - 0090-3558 (Linking) VI - 54 IP - 1 DP - 2018 Jan TI - HOUSE FINCH ( HAEMORHOUS MEXICANUS)-ASSOCIATED MYCOPLASMA GALLISEPTICUM IDENTIFIED IN LESSER GOLDFINCH ( SPINUS PSALTRIA) AND WESTERN SCRUB JAY ( APHELOCOMA CALIFORNICA) USING STRAIN-SPECIFIC QUANTITATIVE PCR. PG - 180-185 LID - 10.7589/2017-04-079 [doi] AB - : In 1994 Mycoplasma gallisepticum was found to be the etiologic agent of House Finch ( Haemorhous mexicanus) conjunctivitis, a rapidly expanding epidemic caused by a genetically discrete, House Finch-associated strain of M. gallisepticum (HFMG). While most prominent in House Finches, HFMG has been reported in other members of the family Fringillidae, including American Goldfinches ( Spinus tristis), Purple Finches ( Haemorhous purpureus), Pine Grosbeaks ( Pinicola enucleator), and Evening Grosbeaks ( Coccothraustes vespertinus). Herein we report two new potential host species of HFMG strain, the Lesser Goldfinch ( Spinus psaltria), belonging to the Fringillidae family, and the Western (California) Scrub Jay ( Aphelocoma californica), belonging to the Corvidae family. The latter is one of only two reports of HFMG being found outside the Fringillidae family, and of these is the only one reported outside of captivity. Furthermore, non-HFMG M. gallisepticum was identified in an American Crow ( Corvus brachyrhynchos), indicating presence of additional strains in wild birds. Strain typing of M. gallisepticum isolates was done via HFMG-specific quantitative PCR analysis and validated using random amplified polymorphic DNA analysis. Our results suggested an expanded host range of HFMG strain, and further suggested that the host range of HFMG was not limited to members of the family Fringillidae. FAU - Allen, Catherine R AU - Allen CR AD - 1 Department of Pathobiology and Veterinary Science and Center of Excellence for Vaccine Research, University of Connecticut, 61 N Eagleville Road, Unit 3089, Storrs, Connecticut 06269, USA. FAU - Mara, Arlind AU - Mara A AD - 1 Department of Pathobiology and Veterinary Science and Center of Excellence for Vaccine Research, University of Connecticut, 61 N Eagleville Road, Unit 3089, Storrs, Connecticut 06269, USA. FAU - Tulman, Edan R AU - Tulman ER AD - 1 Department of Pathobiology and Veterinary Science and Center of Excellence for Vaccine Research, University of Connecticut, 61 N Eagleville Road, Unit 3089, Storrs, Connecticut 06269, USA. FAU - Ley, David H AU - Ley DH AD - 2 Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, Raleigh, North Carolina 27607, USA. FAU - Geary, Steven J AU - Geary SJ AD - 1 Department of Pathobiology and Veterinary Science and Center of Excellence for Vaccine Research, University of Connecticut, 61 N Eagleville Road, Unit 3089, Storrs, Connecticut 06269, USA. LA - eng PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20171020 PL - United States TA - J Wildl Dis JT - Journal of wildlife diseases JID - 0244160 RN - 0 (DNA, Bacterial) SB - IM MH - Animals MH - Bird Diseases/*microbiology MH - DNA, Bacterial/genetics MH - Mycoplasma Infections/microbiology/*veterinary MH - Mycoplasma gallisepticum/genetics/*isolation & purification MH - *Passeriformes MH - Polymerase Chain Reaction/*methods OTO - NOTNLM OT - Corvidae OT - House Finch OT - Lesser Goldfinch OT - Mycoplasma gallisepticum OT - RAPD OT - Western Scrub Jay OT - mycoplasmal conjunctivitis OT - qPCR EDAT- 2017/10/21 06:00 MHDA- 2018/10/03 06:00 CRDT- 2017/10/21 06:00 PHST- 2017/10/21 06:00 [pubmed] PHST- 2018/10/03 06:00 [medline] PHST- 2017/10/21 06:00 [entrez] AID - 10.7589/2017-04-079 [doi] PST - ppublish SO - J Wildl Dis. 2018 Jan;54(1):180-185. doi: 10.7589/2017-04-079. Epub 2017 Oct 20.