PMID- 29054133
OWN - NLM
STAT- MEDLINE
DCOM- 20190705
LR  - 20190705
IS  - 1557-9042 (Electronic)
IS  - 0897-7151 (Linking)
VI  - 35
IP  - 3
DP  - 2018 Feb 1
TI  - Effects of Intrathecal Injection of the Conditioned Medium from Bone Marrow 
      Stromal Cells on Spinal Cord Injury in Rats.
PG  - 521-532
LID - 10.1089/neu.2017.5201 [doi]
AB  - Bone marrow stromal cells (BMSCs) have been studied for the treatment of spinal 
      cord injury (SCI). In previous studies, we showed that the transplantation of 
      BMSCs, even though they disappeared from the host spinal cord within 1-3 weeks 
      after transplantation, improved locomotor behaviors and promoted axonal 
      regeneration. This result led to the hypothesis that BMSCs might release some 
      neurotrophic factors effective for the treatment of SCI. The present study 
      examined this by injecting the conditioned medium (CM) of BMSCs to treat SCI in 
      rats. The spinal cord was contusion-injured, followed immediately by continuous 
      injection for 2 weeks of the CM of BMSCs through the cerebrospinal fluid via the 
      4th ventricle using an Alzet osmotic pump. Locomotor behaviors evaluated by the 
      Basso-Beattie-Bresnahan score were markedly improved in the CM-injection group, 
      compared with the control group, at 1 to 4 weeks post-injection. The 
      contusion-injured site of the spinal cord was identified as an astrocyte-devoid 
      area, which contained no astrocytes but was filled with collagen matrices and 
      empty cavities of various sizes. Collagen matrices contained type I collagen and 
      laminin. Numerous axons extended through the collagen matrices of the 
      astrocyte-devoid area. Axons were surrounded by Schwann cells, exhibiting the 
      same morphological characteristics as peripheral nerve fibers. The density of 
      axons extending through the astrocyte-devoid area was higher in the CM-injection 
      group, compared with the control group. CM injection had beneficial effects on 
      locomotor improvements and tissue repair, including axonal regeneration, meaning 
      that the BMSC-CM stimulated the intrinsic ability of the spinal cord to 
      regenerate. Activation of the intrinsic ability of the spinal cord to regenerate 
      by the injection of neurotrophic factors such as BMSC-CM is considered to be a 
      safe and preferable method for the clinical treatment of SCI.
FAU - Kanekiyo, Kenji
AU  - Kanekiyo K
AD  - 1 Central Biomedical Laboratory, Aino University School of Health Science , 
      Osaka, Japan .
FAU - Wakabayashi, Tamami
AU  - Wakabayashi T
AD  - 1 Central Biomedical Laboratory, Aino University School of Health Science , 
      Osaka, Japan .
FAU - Nakano, Norihiko
AU  - Nakano N
AD  - 1 Central Biomedical Laboratory, Aino University School of Health Science , 
      Osaka, Japan .
FAU - Yamada, Yoshihiro
AU  - Yamada Y
AD  - 2 Department of Physical Therapy, Aino University School of Health Science , 
      Osaka, Japan .
FAU - Tamachi, Masahiro
AU  - Tamachi M
AD  - 2 Department of Physical Therapy, Aino University School of Health Science , 
      Osaka, Japan .
FAU - Suzuki, Yoshihisa
AU  - Suzuki Y
AD  - 3 Department of Plastic and Reconstructive Surgery, Tazuke Medical Research 
      Institute , Kitano Hospital, Osaka, Japan .
FAU - Fukushima, Masatoshi
AU  - Fukushima M
AD  - 4 Translational Research Informatics Center , Foundation for Biomedical Research 
      and Innovation, Kobe, Japan .
FAU - Saito, Fukuki
AU  - Saito F
AD  - 5 Emergency and Clinical Care Center, Kansai Medical University , Osaka, Japan .
FAU - Abe, Seiya
AU  - Abe S
AD  - 6 Department of Occupational Therapy, Aino University School of Health Science , 
      Osaka, Japan .
FAU - Tsukagoshi, Chihiro
AU  - Tsukagoshi C
AD  - 6 Department of Occupational Therapy, Aino University School of Health Science , 
      Osaka, Japan .
FAU - Miyamoto, Chimi
AU  - Miyamoto C
AD  - 6 Department of Occupational Therapy, Aino University School of Health Science , 
      Osaka, Japan .
FAU - Ide, Chizuka
AU  - Ide C
AD  - 1 Central Biomedical Laboratory, Aino University School of Health Science , 
      Osaka, Japan .
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171120
PL  - United States
TA  - J Neurotrauma
JT  - Journal of neurotrauma
JID - 8811626
RN  - 0 (Culture Media, Conditioned)
SB  - IM
MH  - Animals
MH  - Culture Media, Conditioned/*pharmacology
MH  - Female
MH  - Injections, Spinal
MH  - Mesenchymal Stem Cells/*metabolism
MH  - Nerve Regeneration/*drug effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Recovery of Function/drug effects
MH  - Spinal Cord Injuries/*pathology
OTO - NOTNLM
OT  - axonal regeneration
OT  - bone marrow stromal cell
OT  - conditioned culture medium
OT  - locomotor function
OT  - spinal cord injury
EDAT- 2017/10/22 06:00
MHDA- 2019/07/06 06:00
CRDT- 2017/10/22 06:00
PHST- 2017/10/22 06:00 [pubmed]
PHST- 2019/07/06 06:00 [medline]
PHST- 2017/10/22 06:00 [entrez]
AID - 10.1089/neu.2017.5201 [doi]
PST - ppublish
SO  - J Neurotrauma. 2018 Feb 1;35(3):521-532. doi: 10.1089/neu.2017.5201. Epub 2017 
      Nov 20.