PMID- 29055654 OWN - NLM STAT- MEDLINE DCOM- 20190626 LR - 20210929 IS - 1095-8584 (Electronic) IS - 0022-2828 (Print) IS - 0022-2828 (Linking) VI - 114 DP - 2018 Jan TI - Echocardiographic evaluation of diastolic function in mouse models of heart disease. PG - 20-28 LID - S0022-2828(17)30330-9 [pii] LID - 10.1016/j.yjmcc.2017.10.006 [doi] AB - BACKGROUND: Mouse models of heart disease are extensively employed. The echocardiographic characterization of contractile function is usually focused on systolic function with fewer studies assessing diastolic function. Furthermore, the applicability of diverse echocardiographic parameters of diastolic function that are commonly used in humans has not been extensively evaluated in different pathophysiological models in mice. METHODS AND RESULTS: We used high resolution echocardiography to evaluate parameters of diastolic function in mouse models of chronic pressure overload (aortic constriction), volume overload (aorto-caval shunt), heart failure with preserved ejection fraction (HFpEF; DOCA-salt hypertension), and acute sarcoplasmic reticulum dysfunction induced by thapsigargin - all known to exhibit diastolic dysfunction. Left atrial area increased in all three chronic models while mitral E/A was difficult to quantify at high heart rates. Isovolumic relaxation time (IVRT) and Doppler E/E' increased significantly and the peak longitudinal strain rate during early filling (peak reverse longitudinal strain rate) decreased significantly after aortic constriction, with the changes being proportional to the magnitude of hypertrophy. In the HFpEF model, reverse longitudinal strain rate decreased significantly but changes in IVRT and E/E' were non-significant, consistent with less severe dysfunction. With volume overload, there was a significant increase in reverse longitudinal strain rate and decrease in IVRT, indicating a restrictive physiology. Acute thapsigargin treatment caused significant prolongation of IVRT and decrease in reverse longitudinal strain rate. CONCLUSION: These results indicate that the combined measurement of left atrial area plus reverse longitudinal strain rate and/or IVRT provide an excellent overall assessment of diastolic function in the diseased mouse heart, allowing distinction between different types of pathophysiology. CI - Copyright (c) 2017 The Authors. Published by Elsevier Ltd.. All rights reserved. FAU - Schnelle, Moritz AU - Schnelle M AD - King's College London British Heart Foundation Centre of Excellence, Cardiovascular Division, London, United Kingdom; Department of Cardiology and Pneumology, University Medical Center Goettingen, Goettingen, Germany. FAU - Catibog, Norman AU - Catibog N AD - King's College London British Heart Foundation Centre of Excellence, Cardiovascular Division, London, United Kingdom. FAU - Zhang, Min AU - Zhang M AD - King's College London British Heart Foundation Centre of Excellence, Cardiovascular Division, London, United Kingdom. FAU - Nabeebaccus, Adam A AU - Nabeebaccus AA AD - King's College London British Heart Foundation Centre of Excellence, Cardiovascular Division, London, United Kingdom. FAU - Anderson, Grace AU - Anderson G AD - King's College London British Heart Foundation Centre of Excellence, Cardiovascular Division, London, United Kingdom. FAU - Richards, Daniel A AU - Richards DA AD - King's College London British Heart Foundation Centre of Excellence, Cardiovascular Division, London, United Kingdom. FAU - Sawyer, Greta AU - Sawyer G AD - King's College London British Heart Foundation Centre of Excellence, Cardiovascular Division, London, United Kingdom. FAU - Zhang, Xiaohong AU - Zhang X AD - King's College London British Heart Foundation Centre of Excellence, Cardiovascular Division, London, United Kingdom. FAU - Toischer, Karl AU - Toischer K AD - Department of Cardiology and Pneumology, University Medical Center Goettingen, Goettingen, Germany. FAU - Hasenfuss, Gerd AU - Hasenfuss G AD - Department of Cardiology and Pneumology, University Medical Center Goettingen, Goettingen, Germany. FAU - Monaghan, Mark J AU - Monaghan MJ AD - King's College London British Heart Foundation Centre of Excellence, Cardiovascular Division, London, United Kingdom. FAU - Shah, Ajay M AU - Shah AM AD - King's College London British Heart Foundation Centre of Excellence, Cardiovascular Division, London, United Kingdom. Electronic address: ajay.shah@kcl.ac.uk. LA - eng GR - CH/1999001/11735/BHF_/British Heart Foundation/United Kingdom GR - PG/12/26/29477/BHF_/British Heart Foundation/United Kingdom GR - RG/13/11/30384/BHF_/British Heart Foundation/United Kingdom PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20171019 PL - England TA - J Mol Cell Cardiol JT - Journal of molecular and cellular cardiology JID - 0262322 RN - 67526-95-8 (Thapsigargin) RN - EC 3.6.3.8 (Sarcoplasmic Reticulum Calcium-Transporting ATPases) SB - IM MH - Animals MH - Cardiomegaly/complications/pathology/physiopathology MH - Diastole/*physiology MH - Disease Models, Animal MH - *Echocardiography MH - Heart Diseases/complications/*diagnostic imaging/*physiopathology MH - Heart Failure/complications/pathology/physiopathology MH - Mice, Inbred C57BL MH - Observer Variation MH - Pressure MH - Sarcoplasmic Reticulum Calcium-Transporting ATPases/antagonists & inhibitors/metabolism MH - Stroke Volume MH - Systole/physiology MH - Thapsigargin/pharmacology PMC - PMC5807035 OTO - NOTNLM OT - Diastolic function OT - Echocardiography OT - Hypertrophy OT - Mouse EDAT- 2017/10/23 06:00 MHDA- 2019/06/27 06:00 PMCR- 2018/01/01 CRDT- 2017/10/23 06:00 PHST- 2017/06/19 00:00 [received] PHST- 2017/10/05 00:00 [revised] PHST- 2017/10/18 00:00 [accepted] PHST- 2017/10/23 06:00 [pubmed] PHST- 2019/06/27 06:00 [medline] PHST- 2017/10/23 06:00 [entrez] PHST- 2018/01/01 00:00 [pmc-release] AID - S0022-2828(17)30330-9 [pii] AID - 10.1016/j.yjmcc.2017.10.006 [doi] PST - ppublish SO - J Mol Cell Cardiol. 2018 Jan;114:20-28. doi: 10.1016/j.yjmcc.2017.10.006. Epub 2017 Oct 19.