PMID- 29057768
OWN - NLM
STAT- MEDLINE
DCOM- 20180817
LR  - 20180817
IS  - 1348-4540 (Electronic)
IS  - 0918-8959 (Linking)
VI  - 65
IP  - 1
DP  - 2018 Jan 30
TI  - Ghrelin protects adult rat hippocampal neural stem cells from excessive autophagy 
      during oxygen-glucose deprivation.
PG  - 63-73
LID - 10.1507/endocrj.EJ17-0281 [doi]
AB  - Ghrelin functions as a neuroprotective agent and saves neurons from various 
      insults include ischemic injury. However, it remains to be elucidated whether 
      ghrelin protects neuronal cells against ischemic injury-induced excessive 
      autophagy. Autophagy is required for the maintenance of neural stem cell 
      homeostasis. However, regarding autophagic cell death, it is commonly assumed 
      that excessive autophagy leads to self-elimination of mammalian cells. The 
      purpose of this study was to investigate the potential neuroprotection effects of 
      ghrelin from excessive autophagy in adult rat hippocampal neural stem cells 
      (NSCs). Oxygen-Glucose Deprivation (OGD) strongly induces autophagy in adult rat 
      hippocampal NSCs. Ghrelin treatment inhibited OGD-induced cell death of adult rat 
      hippocampal NSCs assessed by cell-counting-kit-8 assay. Ghrelin also suppressed 
      OGD-induced excessive autophagy activity. The protective effect of ghrelin was 
      accompanied by an increased expression levels of Bcl-2, p-62 and decreased 
      expression level of LC3-II, Beclin-1 by Western blot. Furthermore, ghrelin 
      reduced autophagosome formation and number of GFP-LC3 transfected puncta. In 
      conclusion, our data suggest that ghrelin protects adult rat hippocampal NSCs 
      from excessive autophagy in experimental stroke (oxygen-glucose deprivation) 
      model. Regulating autophagic activity may be a potential optimizing target for 
      promoting adult rat hippocampal NSCs based therapy for stroke.
FAU - Chung, Hyunju
AU  - Chung H
AD  - Department of Core Research Laboratory, Clinical Research Institute, Kyung Hee 
      University Hospital at Gangdong, Seoul, Korea.
FAU - Choi, Junghyun
AU  - Choi J
AD  - Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul, 
      Korea.
FAU - Park, Seungjoon
AU  - Park S
AD  - Department of Pharmacology and Medical Research Center for Bioreaction to ROS and 
      Biomedical Science Institute, School of Medicine, Kyung Hee University, Seoul, 
      Korea.
LA  - eng
PT  - Journal Article
DEP - 20171021
PL  - Japan
TA  - Endocr J
JT  - Endocrine journal
JID - 9313485
RN  - 0 (Bcl2 protein, rat)
RN  - 0 (Beclin-1)
RN  - 0 (Becn1 protein, rat)
RN  - 0 (Biomarkers)
RN  - 0 (Ghrelin)
RN  - 0 (LC3 protein, rat)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Sequestosome-1 Protein)
RN  - 0 (Sqstm1 protein, rat)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Adult Stem Cells/cytology/metabolism/pathology/ultrastructure
MH  - Animals
MH  - Apoptosis
MH  - *Autophagy
MH  - Beclin-1/antagonists & inhibitors/metabolism
MH  - Biomarkers/metabolism
MH  - Cell Hypoxia
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Ghrelin/*metabolism
MH  - Green Fluorescent Proteins/chemistry/genetics/metabolism
MH  - Hippocampus/cytology/*metabolism/pathology/ultrastructure
MH  - Hypoglycemia/metabolism/pathology
MH  - Microscopy, Electron, Transmission
MH  - Microtubule-Associated Proteins/antagonists & 
      inhibitors/chemistry/genetics/metabolism
MH  - Nerve Tissue Proteins/agonists/antagonists & inhibitors/genetics/metabolism
MH  - Neural Stem Cells/cytology/*metabolism/pathology/ultrastructure
MH  - *Neuroprotection
MH  - Proto-Oncogene Proteins c-bcl-2/*agonists/metabolism
MH  - Rats, Inbred F344
MH  - Recombinant Fusion Proteins/chemistry/metabolism
MH  - Sequestosome-1 Protein/*agonists/metabolism
OTO - NOTNLM
OT  - Excessive autophagy
OT  - Ghrelin
OT  - Hippocampus
OT  - Neuroprotection
OT  - Oxygen-glucose deprivation
EDAT- 2017/10/24 06:00
MHDA- 2018/08/18 06:00
CRDT- 2017/10/24 06:00
PHST- 2017/10/24 06:00 [pubmed]
PHST- 2018/08/18 06:00 [medline]
PHST- 2017/10/24 06:00 [entrez]
AID - 10.1507/endocrj.EJ17-0281 [doi]
PST - ppublish
SO  - Endocr J. 2018 Jan 30;65(1):63-73. doi: 10.1507/endocrj.EJ17-0281. Epub 2017 Oct 
      21.