PMID- 29057951
OWN - NLM
STAT- MEDLINE
DCOM- 20190712
LR  - 20190712
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 7
IP  - 1
DP  - 2017 Oct 20
TI  - Preconditioning with far-infrared irradiation enhances proliferation, cell 
      survival, and migration of rat bone marrow-derived stem cells via CXCR4-ERK 
      pathways.
PG  - 13718
LID - 10.1038/s41598-017-14219-w [doi]
LID - 13718
AB  - Far-infrared radiation (FIR) has been shown to exert positive effects on the 
      cardiovascular system. However, the biological effects of FIR on bone 
      marrow-derived stem cells (BMSCs) are not understood. In the present study, BMSCs 
      were isolated from rat femur bone marrow and cultured in vitro. To investigate 
      the effects of an FIR generator with an energy flux of 0.13 mW/cm(2) on rat 
      BMSCs, survival of BMSCs was measured by crystal violet staining, and cell 
      proliferation was additionally measured using Ez-Cytox cell viability, EdU, and 
      Brd U assays. FIR preconditioning was found to significantly increase BMSC 
      proliferation and survival against H(2)O(2). The scratch and transwell migration 
      assays showed that FIR preconditioning resulted in an increase in BMSC migration. 
      qRT-PCR and Western blot analyses demonstrated that FIR upregulated Nanog, Sox2, 
      c-Kit, Nkx2.5, and CXCR4 at both the mRNA and protein levels. Consistent with 
      these observations, PD98059 (an ERK inhibitor) and AMD3100 (a CXCR4 inhibitor) 
      prevented the activation of CXCR4/ERK and blocked the cell proliferation and 
      migration induced by FIR. Overall, these findings provide the first evidence that 
      FIR confers a real and significant benefit on the preconditioning of BMSCs, and 
      might lead to novel strategies for improving BMSC therapy for cardiac ischemia.
FAU - Jeong, Yun-Mi
AU  - Jeong YM
AD  - Division of Cardiology, Department of Internal Medicine, Kyung Hee University 
      Hospital, Kyung Hee University, Seoul, Republic of Korea.
FAU - Cheng, Xian Wu
AU  - Cheng XW
AD  - Division of Cardiology, Department of Internal Medicine, Kyung Hee University 
      Hospital, Kyung Hee University, Seoul, Republic of Korea.
AD  - The Department of Cardiology, Yanbian University Hospital, Yanji, China.
FAU - Lee, Sora
AU  - Lee S
AD  - Division of Cardiology, Department of Internal Medicine, Kyung Hee University 
      Hospital, Kyung Hee University, Seoul, Republic of Korea.
FAU - Lee, Kyung Hye
AU  - Lee KH
AD  - Division of Cardiology, Department of Internal Medicine, Kyung Hee University 
      Hospital, Kyung Hee University, Seoul, Republic of Korea.
FAU - Cho, Haneul
AU  - Cho H
AD  - Division of Cardiology, Department of Internal Medicine, Kyung Hee University 
      Hospital, Kyung Hee University, Seoul, Republic of Korea.
FAU - Kang, Jung Hee
AU  - Kang JH
AD  - Division of Cardiology, Department of Internal Medicine, Kyung Hee University 
      Hospital, Kyung Hee University, Seoul, Republic of Korea.
FAU - Kim, Weon
AU  - Kim W
AD  - Division of Cardiology, Department of Internal Medicine, Kyung Hee University 
      Hospital, Kyung Hee University, Seoul, Republic of Korea. mylovekw@hanmail.net.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171020
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Cxcr4 protein, rat)
RN  - 0 (Receptors, CXCR4)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
SB  - IM
MH  - Animals
MH  - Apoptosis/physiology/radiation effects
MH  - Bone Marrow Cells/metabolism/*radiation effects
MH  - Cell Movement/physiology/*radiation effects
MH  - Cell Proliferation/physiology/*radiation effects
MH  - Cell Survival/physiology/*radiation effects
MH  - Cells, Cultured
MH  - Dose-Response Relationship, Radiation
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Femur
MH  - Gene Expression Regulation/radiation effects
MH  - Hydrogen Peroxide/administration & dosage/metabolism
MH  - *Infrared Rays
MH  - MAP Kinase Signaling System/radiation effects
MH  - Male
MH  - Rats, Sprague-Dawley
MH  - Receptors, CXCR4/metabolism
MH  - Time Factors
PMC - PMC5651919
COIS- The authors declare that they have no competing interests.
EDAT- 2017/10/24 06:00
MHDA- 2019/07/13 06:00
PMCR- 2017/10/20
CRDT- 2017/10/24 06:00
PHST- 2017/05/03 00:00 [received]
PHST- 2017/10/06 00:00 [accepted]
PHST- 2017/10/24 06:00 [entrez]
PHST- 2017/10/24 06:00 [pubmed]
PHST- 2019/07/13 06:00 [medline]
PHST- 2017/10/20 00:00 [pmc-release]
AID - 10.1038/s41598-017-14219-w [pii]
AID - 14219 [pii]
AID - 10.1038/s41598-017-14219-w [doi]
PST - epublish
SO  - Sci Rep. 2017 Oct 20;7(1):13718. doi: 10.1038/s41598-017-14219-w.