PMID- 29059705
OWN - NLM
STAT- MEDLINE
DCOM- 20190508
LR  - 20190508
IS  - 1552-4957 (Electronic)
IS  - 1552-4949 (Linking)
VI  - 94
IP  - 3
DP  - 2018 May
TI  - Low synovial double negative T and gammadelta T cells predict longer free-disease 
      survival in oligoarticular JIA.
PG  - 423-427
LID - 10.1002/cyto.b.21597 [doi]
AB  - BACKGROUND: Oligoarticular juvenile idiopathic arthritis (oJIA) is the most 
      frequent form of chronic arthritis in children; the clinical course is extremely 
      variable. In this study we have characterized by flow cytometry synovial B and T 
      cells subsets in patients with oJIA in order to identify any parameters that 
      could predict a more aggressive course of disease. METHODS: B and T cells from 
      synovial fluid (SF) of 39 patients with oJIA were characterized by flow 
      cytometry. In 22 patients SF was analysed at the onset of the disease (GroupA), 
      in 17 SF was analysed at articular relapse (Group B). All patients in Group A 
      were followed up for at least for 2 years after SF analysis: 13 patients relapsed 
      during the follow-up period. RESULTS: Comparison of SF from Group A and Group B 
      demonstrated an activated phenotype in relapsed patients, with higher Switched 
      Memory B cells (58.53 vs 36.07% of CD19+, P-value 0.004) and lower Naive B cells 
      (8.53 vs 25.9 of CD19+, P-value 0.002) in Group B. Furthermore, patients from 
      Group A who did not relapse showed lower percentages of synovial DNT (2.38 vs 
      1.50% of CD3 + TCRalpha/beta+, P-value 0.025) and gammadelta T cells (19.1 vs 15.0% of 
      CD3+ cells, P-value 0.004) at the onset, if compared with other Group A patients. 
      CONCLUSIONS: In oJIA relapse SF present an activated B phenotype. Patients at 
      disease onset with DNTs <1.8% and/or gammadelta T cells <16% of CD3+ in synovial fluid 
      have longer free-disease survival. (c) 2017 International Clinical Cytometry 
      Society.
CI  - (c) 2017 International Clinical Cytometry Society.
FAU - Licciardi, Francesco
AU  - Licciardi F
AD  - Department of Pediatrics, Citta della Salute e della Scienza, Regina Margherita 
      Children Hospital, Turin, Italy.
FAU - Ceci, Maria
AU  - Ceci M
AD  - Department of Pediatrics, Citta della Salute e della Scienza, Regina Margherita 
      Children Hospital, Turin, Italy.
FAU - Toppino, Claudia
AU  - Toppino C
AD  - Department of Pediatrics, Citta della Salute e della Scienza, Regina Margherita 
      Children Hospital, Turin, Italy.
FAU - Turco, Marco
AU  - Turco M
AD  - Department of Pediatrics, Citta della Salute e della Scienza, Regina Margherita 
      Children Hospital, Turin, Italy.
FAU - Martino, Silvana
AU  - Martino S
AD  - Department of Pediatrics, Citta della Salute e della Scienza, Regina Margherita 
      Children Hospital, Turin, Italy.
FAU - Ricotti, Emanuela
AU  - Ricotti E
AD  - Department of Pediatrics, Citta della Salute e della Scienza, Regina Margherita 
      Children Hospital, Turin, Italy.
FAU - Ferro, Francesca
AU  - Ferro F
AD  - Department of Immunohaematology, Cardinal Massaia Hospital, Asti, Italy.
FAU - Montin, Davide
AU  - Montin D
AD  - Department of Pediatrics, Citta della Salute e della Scienza, Regina Margherita 
      Children Hospital, Turin, Italy.
LA  - eng
PT  - Journal Article
DEP - 20171115
PL  - United States
TA  - Cytometry B Clin Cytom
JT  - Cytometry. Part B, Clinical cytometry
JID - 101235690
RN  - 0 (Antigens, CD19)
SB  - IM
MH  - Antigens, CD19/immunology
MH  - Arthritis, Juvenile/*immunology/*mortality
MH  - B-Lymphocytes/immunology
MH  - Child
MH  - Child, Preschool
MH  - Disease-Free Survival
MH  - Female
MH  - Flow Cytometry/methods
MH  - Humans
MH  - Male
MH  - Synovial Fluid/*immunology
MH  - T-Lymphocyte Subsets/*immunology
OTO - NOTNLM
OT  - DNT
OT  - JIA
OT  - prognostic factor
OT  - gammadelta T cells
EDAT- 2017/10/24 06:00
MHDA- 2019/05/09 06:00
CRDT- 2017/10/24 06:00
PHST- 2017/06/15 00:00 [received]
PHST- 2017/09/20 00:00 [revised]
PHST- 2017/10/18 00:00 [accepted]
PHST- 2017/10/24 06:00 [pubmed]
PHST- 2019/05/09 06:00 [medline]
PHST- 2017/10/24 06:00 [entrez]
AID - 10.1002/cyto.b.21597 [doi]
PST - ppublish
SO  - Cytometry B Clin Cytom. 2018 May;94(3):423-427. doi: 10.1002/cyto.b.21597. Epub 
      2017 Nov 15.