PMID- 29062719
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220129
IS  - 2215-0161 (Print)
IS  - 2215-0161 (Electronic)
IS  - 2215-0161 (Linking)
VI  - 4
DP  - 2017
TI  - A highly sensitive liquid chromatography electrospray ionization mass 
      spectrometry method for quantification of TMA, TMAO and creatinine in mouse 
      urine.
PG  - 310-319
LID - 10.1016/j.mex.2017.09.004 [doi]
AB  - Our method describes the quantification in mouse urine of trimethylamine (TMA), 
      trimethylamine N-oxide (TMAO) and creatinine. The method combines derivatization 
      of TMA, with ethyl bromoacetate, and LC chromatographic separation on an ACE 
      C(18) column. The effluent was continuously electrosprayed into the linear ion 
      trap mass spectrometer (LTQ), which operated in selective ion monitoring (SIM) 
      modes set for targeted analytes and their internal standards (IS). All validation 
      parameters were within acceptable ranges of analytical method validation 
      guidelines. Intra- and inter-day assay precision and accuracy coefficients of 
      variation were <3.1%, and recoveries for TMA and TMAO were 97-104%. The method 
      developed uses a two-step procedure. Firstly, TMA and TMAO are analyzed without a 
      purification step using a 5-min gradient cap-LC- SIMs analysis, then creatinine 
      is analyzed using the same experimental conditions. The method is robust, highly 
      sensitive, reproducible and has the high-throughput capability of detecting TMA, 
      TMAO and creatinine at on-column concentrations as low as 28 pg/mL, 115 pg/mL and 
      1 ng/mL, respectively. The method is suitable for analysis of TMA, TMAO and 
      creatinine in both male and female mouse urine. The key benefits of the method 
      are: *The small sample volume of urine required, which overcomes the difficulties 
      of collecting sufficient volumes of urine at defined times.*No sample 
      pre-treatment is necessary.*The quantification of TMA, TMAO and creatinine using 
      the same cap-LC-MS method.
FAU - Veeravalli, Sunil
AU  - Veeravalli S
AD  - Institute of Structural and Molecular Biology, University College London, London, 
      UK.
FAU - Karu, Kersti
AU  - Karu K
AD  - Mass Spectrometry Facility, Department of Chemistry, University College London, 
      London, UK.
FAU - Phillips, Ian R
AU  - Phillips IR
AD  - Institute of Structural and Molecular Biology, University College London, London, 
      UK.
AD  - School of Biological and Chemical Sciences, Queen Mary University of London, 
      London, UK.
FAU - Shephard, Elizabeth A
AU  - Shephard EA
AD  - Institute of Structural and Molecular Biology, University College London, London, 
      UK.
LA  - eng
GR  - MR/K015494/1/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
DEP - 20170928
PL  - Netherlands
TA  - MethodsX
JT  - MethodsX
JID - 101639829
PMC - PMC5643081
OTO - NOTNLM
OT  - Creatinine using LC-ESI-MS
OT  - Electrospray ionization
OT  - FMO3
OT  - Liquid Chromatography
OT  - Mass spectrometry
OT  - Mouse urine
OT  - Quantification of TMA
OT  - TMAO
OT  - Trimethylamine
OT  - Trimethylamine N-oxide
EDAT- 2017/10/25 06:00
MHDA- 2017/10/25 06:01
PMCR- 2017/09/28
CRDT- 2017/10/25 06:00
PHST- 2017/05/10 00:00 [received]
PHST- 2017/09/18 00:00 [accepted]
PHST- 2017/10/25 06:00 [entrez]
PHST- 2017/10/25 06:00 [pubmed]
PHST- 2017/10/25 06:01 [medline]
PHST- 2017/09/28 00:00 [pmc-release]
AID - S2215-0161(17)30034-1 [pii]
AID - 10.1016/j.mex.2017.09.004 [doi]
PST - epublish
SO  - MethodsX. 2017 Sep 28;4:310-319. doi: 10.1016/j.mex.2017.09.004. eCollection 
      2017.