PMID- 2906296
OWN - NLM
STAT- MEDLINE
DCOM- 19890329
LR  - 20190721
IS  - 0014-4819 (Print)
IS  - 0014-4819 (Linking)
VI  - 73
IP  - 3
DP  - 1988
TI  - Structural requirements for activation of excitatory amino acid receptors in the 
      rat spinal cord in vitro.
PG  - 541-5
AB  - The conformational requirements for activation of N-methyl-D-aspartate (NMDA) and 
      quisqualate (QUIS) excitatory amino acid receptors on rat spinal neurones in 
      vitro have been examined using a number of conformationally restricted compounds 
      related to L-glutamate (L-GLU). The excitants were assigned to a receptor type on 
      the basis of their susceptibility to blockade by D (-)-2-amino-5-phosphonvalerate 
      (DAPV) and kynurenate (KYNA). When iontophoretically applied to unidentified 
      neurones in the dorsal horn of spinal cord slices maintained in vitro, three of 
      the isomers of 1-amino-1,3-cyclopentane dicarboxylate (ACPD) evoked excitations 
      which were DAPV-sensitive and therefore were probably elicited via NMDA 
      receptors. The fourth isomer (D-trans-(1R,3S)-ACPD) resembled quinolinate (QUIN) 
      in its actions, and differed from both NMDA and QUIS. Several pyridine 
      derivatives in addition to QUIN were tested, and both the 2,5- and 2,6-pyridine 
      dicarboxylates evoked excitations which, like those produced by QUIS and L-GLU, 
      were largely unaffected by both DAPV and KYNA and thus appeared due to activation 
      of the QUIS receptor. 2,4-Pyridine dicarboxylate acted as a weak and unselective 
      antagonist of amino acid-induced excitations. The results support an earlier 
      conclusion that compounds reacting with the NMDA receptor do so in an extended 
      configuration whereas the QUIS receptor has a more folded template. The 
      possibility that QUIN reacts with a receptor different from those activated by 
      other amino acids is considered.
FAU - Magnuson, D S
AU  - Magnuson DS
AD  - Department of Physiology, University of British Columbia, Vancouver, Canada.
FAU - Curry, K
AU  - Curry K
FAU - Peet, M J
AU  - Peet MJ
FAU - McLennan, H
AU  - McLennan H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Exp Brain Res
JT  - Experimental brain research
JID - 0043312
RN  - 0 (Receptors, AMPA)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 0 (Receptors, Neurotransmitter)
RN  - 76726-92-6 (2-Amino-5-phosphonovalerate)
RN  - H030S2S85J (Kynurenic Acid)
RN  - HG18B9YRS7 (Valine)
SB  - IM
MH  - 2-Amino-5-phosphonovalerate
MH  - Action Potentials/drug effects
MH  - Animals
MH  - In Vitro Techniques
MH  - Kynurenic Acid/pharmacology
MH  - Male
MH  - Protein Conformation
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Receptors, AMPA
MH  - Receptors, N-Methyl-D-Aspartate
MH  - Receptors, Neurotransmitter/drug effects/*metabolism
MH  - Spinal Cord/drug effects/*metabolism
MH  - Valine/analogs & derivatives/pharmacology
EDAT- 1988/01/01 00:00
MHDA- 1988/01/01 00:01
CRDT- 1988/01/01 00:00
PHST- 1988/01/01 00:00 [pubmed]
PHST- 1988/01/01 00:01 [medline]
PHST- 1988/01/01 00:00 [entrez]
AID - 10.1007/BF00406612 [doi]
PST - ppublish
SO  - Exp Brain Res. 1988;73(3):541-5. doi: 10.1007/BF00406612.