PMID- 29066283
OWN - NLM
STAT- MEDLINE
DCOM- 20180326
LR  - 20180920
IS  - 0925-4439 (Print)
IS  - 0925-4439 (Linking)
VI  - 1864
IP  - 1
DP  - 2018 Jan
TI  - The extent of pyroptosis varies in different stages of apical periodontitis.
PG  - 226-237
LID - S0925-4439(17)30389-7 [pii]
LID - 10.1016/j.bbadis.2017.10.025 [doi]
AB  - Apical periodontitis (AP) is an inflammation affecting the periapical region of 
      tooth root. Microbial pathogens activate inflammasomes and promote the production 
      of pro-inflammatory cytokines. Caspase-1-mediated pyroptosis is a possible 
      mechanism involved in the initiation and progression of AP. The purpose of this 
      study was to evaluate the role of caspase-1 and pyroptosis on AP at different 
      stages. Human periapical inflammatory tissue was collected to study chronic AP 
      stage. Human periodontal ligament fibroblasts (hPDLFs) were stimulated with 
      lipopolysaccharide in vitro for 24h to simulate early AP stage. Experimental AP 
      rat model was established to study acute AP stage from 0d to 28d. The results 
      showed that NLRP3, cleaved caspase-1 and Interleukin (IL)-1beta were enhanced in all 
      AP stages. Caspase-1 activation was detectable in most cells. However, the level 
      of pyroptosis was in accordance with the degree of AP inflammation. Early and 
      chronic AP showed a comparable hemostasis state, with pyroptosis remaining in a 
      reduced level. On the contrary, extensive pyroptosis accelerated inflammation and 
      induced cell death in acute AP. VX765, a caspase-1 inhibitor, was used in an 
      experimental AP rat model. The results showed that VX765 suppressed bone loss, 
      suggesting a role of pyroptosis on bone resorption in acute AP. VX765 also 
      inhibited the expressions of IL-1beta, Monocyte chemoattractant protein-1 (MCP-1), 
      IL-6 and IL-8 in vitro, thus decreased inflammatory responses during AP. In 
      conclusion, caspase-1 and pyroptosis contributed to AP inflammation and lesion 
      and pyroptosis extent was in line with AP progression.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Cheng, Ran
AU  - Cheng R
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, Dept. of Preventive Dentistry, West China Hospital of Stomatology, 
      Sichuan University, Chengdu, Sichuan, China; State Key Laboratory of Oral 
      Diseases, National Clinical Research Center for Oral Diseases, Dept. of Cariology 
      and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, 
      Sichuan, China.
FAU - Feng, Yuchao
AU  - Feng Y
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, Dept. of Cariology and Endodontics, West China Hospital of Stomatology, 
      Sichuan University, Chengdu, Sichuan, China.
FAU - Zhang, Rui
AU  - Zhang R
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, Dept. of Preventive Dentistry, West China Hospital of Stomatology, 
      Sichuan University, Chengdu, Sichuan, China; State Key Laboratory of Oral 
      Diseases, National Clinical Research Center for Oral Diseases, Dept. of Cariology 
      and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, 
      Sichuan, China.
FAU - Liu, Wen
AU  - Liu W
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, Dept. of Cariology and Endodontics, West China Hospital of Stomatology, 
      Sichuan University, Chengdu, Sichuan, China.
FAU - Lei, Lei
AU  - Lei L
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, Dept. of Preventive Dentistry, West China Hospital of Stomatology, 
      Sichuan University, Chengdu, Sichuan, China; State Key Laboratory of Oral 
      Diseases, National Clinical Research Center for Oral Diseases, Dept. of Cariology 
      and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, 
      Sichuan, China.
FAU - Hu, Tao
AU  - Hu T
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, Dept. of Preventive Dentistry, West China Hospital of Stomatology, 
      Sichuan University, Chengdu, Sichuan, China; State Key Laboratory of Oral 
      Diseases, National Clinical Research Center for Oral Diseases, Dept. of Cariology 
      and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, 
      Sichuan, China. Electronic address: hutao@scu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171021
PL  - Netherlands
TA  - Biochim Biophys Acta Mol Basis Dis
JT  - Biochimica et biophysica acta. Molecular basis of disease
JID - 101731730
RN  - EC 3.4.22.36 (Caspase 1)
SB  - IM
MH  - Animals
MH  - Caspase 1/physiology
MH  - Cells, Cultured
MH  - Disease Progression
MH  - Female
MH  - Fibroblasts/pathology/physiology
MH  - Humans
MH  - Periapical Periodontitis/*pathology
MH  - Periodontal Ligament/pathology
MH  - Pyroptosis/*physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Severity of Illness Index
OTO - NOTNLM
OT  - Caspase-1
OT  - IL-1beta
OT  - Microbial-cell interaction
OT  - Pyroptosis
OT  - hPDLFs
EDAT- 2017/10/27 06:00
MHDA- 2018/03/27 06:00
CRDT- 2017/10/26 06:00
PHST- 2017/08/15 00:00 [received]
PHST- 2017/10/02 00:00 [revised]
PHST- 2017/10/19 00:00 [accepted]
PHST- 2017/10/27 06:00 [pubmed]
PHST- 2018/03/27 06:00 [medline]
PHST- 2017/10/26 06:00 [entrez]
AID - S0925-4439(17)30389-7 [pii]
AID - 10.1016/j.bbadis.2017.10.025 [doi]
PST - ppublish
SO  - Biochim Biophys Acta Mol Basis Dis. 2018 Jan;1864(1):226-237. doi: 
      10.1016/j.bbadis.2017.10.025. Epub 2017 Oct 21.