PMID- 29067030
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1664-462X (Print)
IS  - 1664-462X (Electronic)
IS  - 1664-462X (Linking)
VI  - 8
DP  - 2017
TI  - Physical Location of New PCR-Based Markers and Powdery Mildew Resistance Gene(s) 
      on Rye (Secale cereale L.) Chromosome 4 Using 4R Dissection Lines.
PG  - 1716
LID - 10.3389/fpls.2017.01716 [doi]
LID - 1716
AB  - Rye (Secale cereale L.) 4R chromosome contains elite genes that are applicable 
      for wheat (Triticum aestivum L.) cultivar improvement. PCR-based 4R-specific 
      markers can benefit the detection of elite genes on 4R in wheat backgrounds. In 
      this study, a new fluorescence in situ hybridization (FISH) map of the 4R(Ku) 
      chromosome of rye Kustro has been constructed. A set of 4R(Ku) dissection lines 
      was obtained and 301 new 4R(Ku)-specific markers were developed using specific 
      length amplified fragment sequencing (SLAF-seq) technology. These markers were 
      combined with the 99 4R(Ku)-specific markers previously developed, and were 
      physically mapped to 4R(Ku) chromosome using the new FISH map and the 4R(Ku) 
      dissection lines. A total of 338 of the 400 markers have been successfully mapped 
      to six regions of 4R(Ku) chromosome. Additionally, the powdery mildew resistance 
      gene(s) on the 4RL(Ku) arm was located to the segment between L.4 and L.8, the 
      same region where 115 4RL(Ku)-specific markers were mapped. The markers developed 
      in this study can be used to identify a specific segment of 4R chromatin in wheat 
      backgrounds, help construct a high-density physical map of 4R chromosome, and 
      facilitate the utilization of elite genes on 4R chromosome in wheat breeding 
      programs.
FAU - Duan, Qiong
AU  - Duan Q
AD  - Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural 
      University, Chengdu, China.
AD  - Institute of Ecological Agriculture, Sichuan Agricultural University, Chengdu, 
      China.
FAU - Wang, Yang Yang
AU  - Wang YY
AD  - Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural 
      University, Chengdu, China.
AD  - Institute of Ecological Agriculture, Sichuan Agricultural University, Chengdu, 
      China.
FAU - Qiu, Ling
AU  - Qiu L
AD  - Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural 
      University, Chengdu, China.
FAU - Ren, Tian Heng
AU  - Ren TH
AD  - Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural 
      University, Chengdu, China.
FAU - Li, Zhi
AU  - Li Z
AD  - College of Life Sciences, Sichuan Agricultural University, Ya'an, China.
FAU - Fu, Shu Lan
AU  - Fu SL
AD  - Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural 
      University, Chengdu, China.
FAU - Tang, Zong Xiang
AU  - Tang ZX
AD  - Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural 
      University, Chengdu, China.
AD  - Institute of Ecological Agriculture, Sichuan Agricultural University, Chengdu, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20171010
PL  - Switzerland
TA  - Front Plant Sci
JT  - Frontiers in plant science
JID - 101568200
PMC - PMC5641395
OTO - NOTNLM
OT  - 4R dissection line
OT  - PCR-based markers
OT  - physical map
OT  - powdery mildew
OT  - rye
OT  - wheat
EDAT- 2017/10/27 06:00
MHDA- 2017/10/27 06:01
PMCR- 2017/01/01
CRDT- 2017/10/26 06:00
PHST- 2017/06/21 00:00 [received]
PHST- 2017/09/20 00:00 [accepted]
PHST- 2017/10/26 06:00 [entrez]
PHST- 2017/10/27 06:00 [pubmed]
PHST- 2017/10/27 06:01 [medline]
PHST- 2017/01/01 00:00 [pmc-release]
AID - 10.3389/fpls.2017.01716 [doi]
PST - epublish
SO  - Front Plant Sci. 2017 Oct 10;8:1716. doi: 10.3389/fpls.2017.01716. eCollection 
      2017.