PMID- 29071911
OWN - NLM
STAT- MEDLINE
DCOM- 20180713
LR  - 20181202
IS  - 1000-0607 (Print)
IS  - 1000-0607 (Linking)
VI  - 41
IP  - 3
DP  - 2016 Jun 25
TI  - [Effect of Electroacupuncture plus Polysaccharide of Gastrodia elata Blume on 
      Expression of Nestin and Brain Derived Neurotrophic Factor in the Basolateral 
      Amygdala of Focal Cerebral Ischemia Rats].
PG  - 230-4
AB  - OBJECTIVE: To observe the effect of electroacupuncture (EA) combined with 
      administration of Polysaccharide of Gastrodia elata Blume (PGB) on stem cell 
      proliferation in the basolateral amygdala (BLA) of focal cerebral ischemia (FCI) 
      rats. METHODS: A total of 40 Sprague-Dawley rats were randomly divided into 
      normal control,model,EA, PGB and EA+PGB groups (n=8 rats in each group). The FCI 
      model was established by occlusion of the right middle cerebral artery. EA (2 Hz, 
      3 V) was applied to "Baihui" (GV 20) and the left "Zusanli"(ST 36) for 30 min, 
      once daily for 14 successive days. Rats of the PGB and EA+PGB groups were given 
      with PGB (100 mg/kg) by gastrogavage, once daily, for14 successive days. The 
      Zea-Longa's method was used to determine the animals' nerurological dysfunction 
      score on day 1 and 14. The expressions of Nestin and brain derived neurotrophic 
      factor (BDNF) of the right BLA were measured by immunohistochemical staining. 
      RESULTS: Following modeling, the neurological score, and the numbers of Nestin- 
      and BDNF-immunoreaction (IR)-positive cells were significantly increased in the 
      model group (P<0.05). Compared with the model group, the neurological scores were 
      considerably decreased in the EA, PGB and EA+PGB groups (P<0.05), and the numbers 
      of Nestin- and BDNF-IR positive cells were further significantly increased in the 
      3 treatment groups (P<0.05), and the effect of EA+PGB was notably superior to 
      that of simple EA and simple PGB in down-regulating neurological score and 
      up-regulating Nestin and BDNF expression (P<0.05). CONCLUSIONS: Both EA and PGB 
      interventions can improve FCI rats' neurological function, which may be related 
      to their effects in up-regulating the expression of Nestin and BDNF to promote 
      the proliferation of neural stem cells in the BLA.
FAU - Huang, Rui
AU  - Huang R
AD  - Department of Anatomy, Wannan Medical College, Wuhu 241002, China.
FAU - Zhao, Jian
AU  - Zhao J
AD  - Department of Anatomy, Wannan Medical College, Wuhu 241002, China.
FAU - Wu, Feng
AU  - Wu F
AD  - Department of Anatomy, Wannan Medical College, Wuhu 241002, China.
FAU - Miao, Hua-Chun
AU  - Miao HC
AD  - Department of Anatomy, Wannan Medical College, Wuhu 241002, China.
FAU - Ding, Jian
AU  - Ding J
AD  - Department of Anatomy, Wannan Medical College, Wuhu 241002, China.
FAU - Xiong, Ke-Ren
AU  - Xiong KR
AD  - Department of Anatomy, Wannan Medical College, Wuhu 241002, China. xkrn@sohu.com.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhen Ci Yan Jiu
JT  - Zhen ci yan jiu = Acupuncture research
JID - 8507710
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nes protein, rat)
RN  - 0 (Nestin)
RN  - 0 (Polysaccharides)
SB  - IM
MH  - Animals
MH  - Basolateral Nuclear Complex/*metabolism
MH  - Brain Ischemia/*metabolism
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - *Electroacupuncture
MH  - Gastrodia/chemistry
MH  - Nestin/*metabolism
MH  - Polysaccharides/*pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2016/06/25 00:00
MHDA- 2018/07/14 06:00
CRDT- 2017/10/27 06:00
PHST- 2017/10/27 06:00 [entrez]
PHST- 2016/06/25 00:00 [pubmed]
PHST- 2018/07/14 06:00 [medline]
PST - ppublish
SO  - Zhen Ci Yan Jiu. 2016 Jun 25;41(3):230-4.