PMID- 29071912
OWN - NLM
STAT- MEDLINE
DCOM- 20180713
LR  - 20181202
IS  - 1000-0607 (Print)
IS  - 1000-0607 (Linking)
VI  - 41
IP  - 3
DP  - 2016 Jun 25
TI  - [Effect of Warm-needle-moxibustion on Behavior Reactions and TNF-alpha and MMP-3 
      Contents in Knee Cartilage of Rabbits with Knee Osteoarthritis].
PG  - 235-9
AB  - OBJECTIVE: To observe the effect of warm-needle-moxibustion on behavior changes 
      and the levels of tumor necrosis factor-alpha (TNF-alpha) and matrix metalloproteinase-3 
      (MMP-3) in the knee cartilage tissue of rabbits with knee osteoarthritis (KOA) so 
      as to explore its mechanisms in relieving KOA. METHODS: Thirty male Japanese 
      white rabbits were randomly divided into normal, model and treatment groups (n=10 
      in each group). The KOA model was established by using immobilization me-thod. 
      The internal and external "Dubi" (ST 35), "Xuehai" (SP 10) and "Yanglingquan "(GB 
      34) were punctured with filiform needles first, followed by attaching an ignited 
      moxa column to the handle of each inserted needles. The treatment was conducted 
      for 20 min every time, once daily for 4 weeks. The behavioral scores were 
      assessed according to the pain severity, gait, joint motion range and articular 
      swelling severity in reference to the modified Lequesne's methods. The TNF-alpha and 
      MMP-3 contents in the knee joint cartilage tissue were assayed by 
      radioimmunoassay, and pathological changes of the articular cartilage tissue were 
      observed under microscope after sectioning and H.E. staining. RESULTS: The 
      Lequesne total score, and the cartilage TNF-alpha and MMP-3 contents were 
      significantly increased in the model group than in the normal group (P<0.05), and 
      obviously down-regulated after warm-needle-moxibustion intervention (P<0.05). 
      Under light microscope, the inflammatory infiltration of the articular cartilage 
      and the disordered arrangement of chondrocytes in KOA rabbits were improved in 
      the treatment group. CONCLUSIONS: Warm-needle-moxibustion intervention can 
      improve the motor function of rabbits with KOA, which may be related to its 
      effects in reducing the level of TNF-alpha and MMP-3 in articular cartilage tissue.
FAU - Yue, Ping
AU  - Yue P
AD  - School of Acu-moxibustion and Tuina, Beijing University of Chinese Medicine, 
      Beijing 100029, China.
FAU - Gao, Liang
AU  - Gao L
AD  - China Rehabilitation Research Center, Beijing 100068.
FAU - Chen, Mo
AU  - Chen M
AD  - Beijing Massage Hospital, Beijing 100035.
FAU - Guo, Wen
AU  - Guo W
AD  - School of Acu-moxibustion and Tuina, Beijing University of Chinese Medicine, 
      Beijing 100029, Chin.
FAU - Xin, Sui-Cheng
AU  - Xin SC
AD  - School of Acu-moxibustion and Tuina, Beijing University of Chinese Medicine, 
      Beijing 100029, Chin. pro_xinsch@163.com.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhen Ci Yan Jiu
JT  - Zhen ci yan jiu = Acupuncture research
JID - 8507710
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Animals
MH  - Cartilage, Articular/*chemistry
MH  - Knee Joint
MH  - Male
MH  - Matrix Metalloproteinase 3/*analysis
MH  - *Moxibustion
MH  - Osteoarthritis, Knee/*therapy
MH  - Rabbits
MH  - Tumor Necrosis Factor-alpha/*analysis
EDAT- 2016/06/25 00:00
MHDA- 2018/07/14 06:00
CRDT- 2017/10/27 06:00
PHST- 2017/10/27 06:00 [entrez]
PHST- 2016/06/25 00:00 [pubmed]
PHST- 2018/07/14 06:00 [medline]
PST - ppublish
SO  - Zhen Ci Yan Jiu. 2016 Jun 25;41(3):235-9.