PMID- 29074103
OWN - NLM
STAT- MEDLINE
DCOM- 20180720
LR  - 20201209
IS  - 1879-0089 (Electronic)
IS  - 0145-305X (Linking)
VI  - 79
DP  - 2018 Feb
TI  - Molecular identification and functional analysis of two variants of myeloid 
      differentiation factor 88 (MyD88) from disk abalone (Haliotis discus discus).
PG  - 113-127
LID - S0145-305X(17)30470-6 [pii]
LID - 10.1016/j.dci.2017.10.010 [doi]
AB  - Myeloid differentiation factor 88 (MyD88) is a crucial adaptor protein of the 
      Toll-like receptor (TLR)- and interleukin 1 receptor-mediated signaling pathways 
      and is involved in a diverse array of inflammatory responses via NF-kappaB 
      activation. In the present study, two MyD88 variants were identified from disk 
      abalone (Haliotis discus discus) and designated AbMyD88-2 and AbMyD88-X. The 
      deduced AbMyD88-2 and AbMyD88-X comprised 433 and 354 amino acids with predicted 
      molecular masses of 48.85 kDa and 40.17 kDa, respectively. AbMyD88-2 and 
      AbMyD88-X possessed typical MyD88 domain structural features including an 
      N-terminal death domain (DD) and C-terminal toll interleukin 1 receptor (TIR) 
      domain similar to those in mammals. Expression analysis of AbMyD88-2 and 
      AbMyD88-X mRNA at different early embryonic developmental stages of abalone by 
      qPCR revealed that their constitutive expression at all developmental stages 
      analyzed with the considerably higher values at the 16-cell (AbMyD88-2) and 
      morula stages (AbMyD88-X). In unchallenged disk abalones, AbMyD88-2 was highly 
      expressed in muscles, while AbMyD88-X mRNA was predominantly transcribed in 
      hemocytes. Moreover, AbMyD88-2 and AbMyD88-X mRNA were differentially modulated 
      in abalone hemocytes after a challenge with live bacteria (Vibrio 
      parahaemolyticus, Listeria monocytogenes), virus (viral hemorrhagic septicemia 
      virus), and pathogen-associated molecular patterns (lipopolysaccharides and Poly 
      I:C). Overexpression of AbMyD88-2 and AbMyD88-X in HEK293T cells induced the 
      activation of the NF-kappaB promoter. AbMyD88-2 and AbMyD88-X involvement in 
      inflammatory responses was characterized by their overexpression in RAW264.7 
      murine macrophage cells. These results revealed comparatively higher NO (Nitric 
      oxide) production, induction of inflammatory mediator genes (iNOS and COX2), and 
      proinflammatory genes (IL1beta, IL6 and TNFalpha) expression in abalone 
      MyD88s-overexpressing cells than in mock control in the presence or absence of 
      LPS stimulation. Altogether, these results suggest that existence of a 
      MyD88-dependent like signaling pathway in disk abalone and that both AbMyD88-2 
      and AbMyD88-X might be involved in innate immune and inflammatory responses.
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Priyathilaka, Thanthrige Thiunuwan
AU  - Priyathilaka TT
AD  - Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National 
      University, Jeju Self-Governing Province 63243, Republic of Korea.
FAU - Bathige, S D N K
AU  - Bathige SDNK
AD  - Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National 
      University, Jeju Self-Governing Province 63243, Republic of Korea; Sri Lanka 
      Institute of Nanotechnology (SLINTEC), Nanotechnology and Science Park, 
      Mahenwatta, Pitipana, Homagama, Sri Lanka.
FAU - Lee, Seongdo
AU  - Lee S
AD  - Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National 
      University, Jeju Self-Governing Province 63243, Republic of Korea.
FAU - Lee, Jehee
AU  - Lee J
AD  - Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National 
      University, Jeju Self-Governing Province 63243, Republic of Korea. Electronic 
      address: jehee@jejunu.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20171023
PL  - United States
TA  - Dev Comp Immunol
JT  - Developmental and comparative immunology
JID - 7708205
RN  - 0 (Cytokines)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (NF-kappa B)
RN  - 0 (Pathogen-Associated Molecular Pattern Molecules)
RN  - 0 (Protein Isoforms)
SB  - IM
MH  - Animals
MH  - Cloning, Molecular
MH  - Cytokines/metabolism
MH  - Gastropoda/*immunology
MH  - Gene Expression Regulation, Developmental
MH  - HEK293 Cells
MH  - Hemocytes/*physiology
MH  - Humans
MH  - Immunity, Innate
MH  - Listeria monocytogenes/*immunology
MH  - Listeriosis/*immunology
MH  - Mice
MH  - Myeloid Differentiation Factor 88/genetics/*metabolism
MH  - NF-kappa B/genetics/metabolism
MH  - Novirhabdovirus/*immunology
MH  - Pathogen-Associated Molecular Pattern Molecules/immunology
MH  - Promoter Regions, Genetic/genetics
MH  - Protein Domains/genetics
MH  - Protein Isoforms/genetics
MH  - RAW 264.7 Cells
MH  - Rhabdoviridae Infections/*immunology
MH  - Transcriptome
MH  - Vibrio Infections/*immunology
MH  - Vibrio parahaemolyticus/*immunology
OTO - NOTNLM
OT  - Development stages
OT  - Disk abalone
OT  - Inflammatory responses
OT  - Innate immune responses
OT  - MyD88
OT  - NF-kappaB activation
EDAT- 2017/10/28 06:00
MHDA- 2018/07/22 06:00
CRDT- 2017/10/28 06:00
PHST- 2017/09/06 00:00 [received]
PHST- 2017/10/18 00:00 [revised]
PHST- 2017/10/19 00:00 [accepted]
PHST- 2017/10/28 06:00 [pubmed]
PHST- 2018/07/22 06:00 [medline]
PHST- 2017/10/28 06:00 [entrez]
AID - S0145-305X(17)30470-6 [pii]
AID - 10.1016/j.dci.2017.10.010 [doi]
PST - ppublish
SO  - Dev Comp Immunol. 2018 Feb;79:113-127. doi: 10.1016/j.dci.2017.10.010. Epub 2017 
      Oct 23.