PMID- 29075579 OWN - NLM STAT- MEDLINE DCOM- 20180709 LR - 20210109 IS - 2162-3279 (Electronic) VI - 7 IP - 10 DP - 2017 Oct TI - Expression of BDNF and trkB in the hippocampus of a rat genetic model of vulnerability (Roman low-avoidance) and resistance (Roman high-avoidance) to stress-induced depression. PG - e00861 LID - 10.1002/brb3.861 [doi] LID - e00861 AB - INTRODUCTION: The selective breeding of Roman High- (RHA) and Low-Avoidance (RLA) rats for, respectively, rapid versus poor acquisition of the active avoidance response has generated two distinct phenotypes differing in many behavioral traits, including coping strategies to aversive conditions. Thus, RLA rats are considered as a genetic model of vulnerability to stress-induced depression whereas RHA rats are a model of resilience to that trait. Besides the monoamine hypothesis of depression, there is evidence that alterations in neuronal plasticity in the hippocampus and other brain areas are critically involved in the pathophysiology of mood disorders. MATERIALS AND METHODS: Western blot (WB) and immunohistochemistry were used to investigate the basal immunochemical occurrence of brain-derived neurotrophic factor (BDNF) and its high-affinity tyrosine-kinase receptor trkB in the dorsal and ventral hippocampus of adult RHA and RLA rats. RESULTS: WB analysis indicated that the optical density of BDNF- and trkB-positive bands in the dorsal hippocampus is, respectively, 48% and 25% lower in RLA versus RHA rats. Densitometric analysis of BDNF- and trkB-like immunoreactivity (LI) in brain sections showed that BDNF-LI is 24% to 34% lower in the different sectors of the Ammon's horn of RLA versus RHA rats, whereas line-related differences are observed in the dentate gyrus (DG) only in the ventral hippocampus. As for trkB-LI, significant differences are observed only in the dorsal hippocampus, where density is 23% lower in the DG of RLA versus RHA rats, while no differences across lines occur in the Ammon's horn. CONCLUSION: These findings support the hypothesis that a reduced BDNF/trkB signaling in the hippocampus of RLA versus RHA rats may contribute to their more pronounced vulnerability to stress-induced depression. FAU - Serra, M Pina AU - Serra MP AD - Department of Biomedical Sciences University of Cagliari Monserrato (CA) Italy. FAU - Poddighe, Laura AU - Poddighe L AD - Department of Biomedical Sciences University of Cagliari Monserrato (CA) Italy. FAU - Boi, Marianna AU - Boi M AD - Department of Biomedical Sciences University of Cagliari Monserrato (CA) Italy. FAU - Sanna, Francesco AU - Sanna F AD - Department of Life and Environmental Sciences University of Cagliari Cagliari Italy. FAU - Piludu, M Antonietta AU - Piludu MA AD - Department of Life and Environmental Sciences University of Cagliari Cagliari Italy. FAU - Corda, M Giuseppa AU - Corda MG AD - Department of Life and Environmental Sciences University of Cagliari Cagliari Italy. FAU - Giorgi, Osvaldo AU - Giorgi O AD - Department of Life and Environmental Sciences University of Cagliari Cagliari Italy. FAU - Quartu, Marina AU - Quartu M AUID- ORCID: 0000-0002-1884-3597 AD - Department of Biomedical Sciences University of Cagliari Monserrato (CA) Italy. LA - eng PT - Journal Article DEP - 20171023 PL - United States TA - Brain Behav JT - Brain and behavior JID - 101570837 RN - 0 (Brain-Derived Neurotrophic Factor) RN - EC 2.7.10.1 (Ntrk2 protein, rat) RN - EC 2.7.10.1 (Receptor, trkB) SB - IM MH - Animals MH - Brain-Derived Neurotrophic Factor/*genetics MH - *Depression/etiology/genetics/metabolism MH - Hippocampus/*metabolism MH - Immunohistochemistry MH - Male MH - Models, Animal MH - Models, Genetic MH - Neuronal Plasticity/genetics MH - Rats MH - Receptor, trkB/*genetics MH - Temporal Lobe/metabolism PMC - PMC5651403 OTO - NOTNLM OT - Roman rat lines OT - brain-derived neurotrophic factor OT - genetic model of depression OT - hippocampus OT - immunohistochemistry OT - trkB OT - western blot EDAT- 2017/10/28 06:00 MHDA- 2018/07/10 06:00 PMCR- 2017/10/23 CRDT- 2017/10/28 06:00 PHST- 2017/05/10 00:00 [received] PHST- 2017/09/13 00:00 [revised] PHST- 2017/09/22 00:00 [accepted] PHST- 2017/10/28 06:00 [entrez] PHST- 2017/10/28 06:00 [pubmed] PHST- 2018/07/10 06:00 [medline] PHST- 2017/10/23 00:00 [pmc-release] AID - BRB3861 [pii] AID - 10.1002/brb3.861 [doi] PST - epublish SO - Brain Behav. 2017 Oct 23;7(10):e00861. doi: 10.1002/brb3.861. eCollection 2017 Oct.