PMID- 29075862 OWN - NLM STAT- MEDLINE DCOM- 20180702 LR - 20210316 IS - 1439-6327 (Electronic) IS - 1439-6319 (Linking) VI - 117 IP - 12 DP - 2017 Dec TI - Concomitant external pneumatic compression treatment with consecutive days of high intensity interval training reduces markers of proteolysis. PG - 2587-2600 LID - 10.1007/s00421-017-3746-2 [doi] AB - PURPOSE: To compare the effects of external pneumatic compression (EPC) and sham when used concurrently with high intensity interval training (HIIT) on performance-related outcomes and recovery-related molecular measures. METHODS: Eighteen recreationally endurance-trained male participants (age: 21.6 +/- 2.4 years, BMI: 25.7 +/- 0.5 kg/m(2), VO(2peak): 51.3 +/- 0.9 mL/kg/min) were randomized to balanced sham and EPC treatment groups. Three consecutive days of HIIT followed by EPC/sham treatment (Days 2-4) and 3 consecutive days of recovery (Days 5-7) with EPC/sham only on Days 5-6 were employed. Venipuncture, flexibility and pressure-to-pain threshold (PPT) measurements were made throughout. Vastus lateralis muscle was biopsied at PRE (i.e., Day 1), 1-h post-EPC/sham treatment on Day 2 (POST1), and 24-h post-EPC/sham treatment on Day 7 (POST2). 6-km run time trial performance was tested at PRE and POST2. RESULTS: No group x time interaction was observed for flexibility, PPT, or serum measures of creatine kinase (CK), hsCRP, and 8-isoprostane. However, there was a main effect of time for serum CK (p = 0.005). Change from PRE in 6-km run times at POST2 were not significantly different between groups. Significant between-groups differences existed for change from PRE in atrogin-1 mRNA (p = 0.018) at the POST1 time point (EPC: - 19.7 +/- 8.1%, sham: + 7.7 +/- 5.9%) and atrogin-1 protein concentration (p = 0.013) at the POST2 time point (EPC: - 31.8 +/- 7.5%, sham: + 96.0 +/- 34.7%). In addition, change from PRE in poly-Ub proteins was significantly different between groups at both the POST1 (EPC: - 26.0 +/- 10.3%, sham: + 34.8 +/- 28.5%; p = 0.046) and POST2 (EPC: - 33.7 +/- 17.2%, sham: + 21.4 +/- 14.9%; p = 0.037) time points. CONCLUSIONS: EPC when used concurrently with HIIT and in subsequent recovery days reduces skeletal muscle markers of proteolysis. FAU - Haun, Cody T AU - Haun CT AD - School of Kinesiology, Auburn University, Auburn, AL, USA. FAU - Roberts, Michael D AU - Roberts MD AD - School of Kinesiology, Auburn University, Auburn, AL, USA. AD - Department of Cell Biology and Physiology, Edward Via College of Osteopathic Medicine, Auburn Campus, 910 S. Donahue Drive, Auburn, AL, 36832, USA. FAU - Romero, Matthew A AU - Romero MA AD - School of Kinesiology, Auburn University, Auburn, AL, USA. FAU - Osburn, Shelby C AU - Osburn SC AD - School of Kinesiology, Auburn University, Auburn, AL, USA. FAU - Healy, James C AU - Healy JC AD - Department of Cell Biology and Physiology, Edward Via College of Osteopathic Medicine, Auburn Campus, 910 S. Donahue Drive, Auburn, AL, 36832, USA. FAU - Moore, Angelique N AU - Moore AN AD - Department of Cell Biology and Physiology, Edward Via College of Osteopathic Medicine, Auburn Campus, 910 S. Donahue Drive, Auburn, AL, 36832, USA. AD - College of Human Sciences, Auburn University, Auburn, AL, 36849, USA. FAU - Mobley, Christopher B AU - Mobley CB AD - School of Kinesiology, Auburn University, Auburn, AL, USA. FAU - Roberson, Paul A AU - Roberson PA AD - School of Kinesiology, Auburn University, Auburn, AL, USA. FAU - Kephart, Wesley C AU - Kephart WC AD - School of Kinesiology, Auburn University, Auburn, AL, USA. FAU - Mumford, Petey W AU - Mumford PW AD - School of Kinesiology, Auburn University, Auburn, AL, USA. FAU - Goodlett, Michael D AU - Goodlett MD AD - Department of Cell Biology and Physiology, Edward Via College of Osteopathic Medicine, Auburn Campus, 910 S. Donahue Drive, Auburn, AL, 36832, USA. AD - Athletics Department, Auburn University, Auburn, AL, USA. FAU - Pascoe, David D AU - Pascoe DD AD - School of Kinesiology, Auburn University, Auburn, AL, USA. FAU - Martin, Jeffrey S AU - Martin JS AUID- ORCID: 0000-0001-6762-086X AD - School of Kinesiology, Auburn University, Auburn, AL, USA. jmartin@auburn.vcom.edu. AD - Department of Cell Biology and Physiology, Edward Via College of Osteopathic Medicine, Auburn Campus, 910 S. Donahue Drive, Auburn, AL, 36832, USA. jmartin@auburn.vcom.edu. LA - eng PT - Journal Article PT - Randomized Controlled Trial DEP - 20171026 PL - Germany TA - Eur J Appl Physiol JT - European journal of applied physiology JID - 100954790 RN - 0 (Muscle Proteins) RN - 27415-26-5 (8-epi-prostaglandin F2alpha) RN - 9007-41-4 (C-Reactive Protein) RN - B7IN85G1HY (Dinoprost) RN - EC 2.3.2.27 (FBXO32 protein, human) RN - EC 2.3.2.27 (SKP Cullin F-Box Protein Ligases) RN - EC 2.7.3.2 (Creatine Kinase) SB - IM MH - Adult MH - C-Reactive Protein/metabolism MH - Creatine Kinase/blood MH - Dinoprost/analogs & derivatives/blood MH - High-Intensity Interval Training/adverse effects/*methods MH - Humans MH - Intermittent Pneumatic Compression Devices/*adverse effects MH - Male MH - Muscle Proteins/metabolism MH - *Proteolysis MH - Quadriceps Muscle/blood supply/metabolism/physiology MH - SKP Cullin F-Box Protein Ligases/metabolism MH - Ubiquitination OTO - NOTNLM OT - Endurance exercise OT - High intensity interval training OT - Oxidative stress OT - Pneumatic compression OT - Proteolysis OT - Recovery OT - Skeletal muscle EDAT- 2017/10/28 06:00 MHDA- 2018/07/03 06:00 CRDT- 2017/10/28 06:00 PHST- 2017/07/01 00:00 [received] PHST- 2017/10/18 00:00 [accepted] PHST- 2017/10/28 06:00 [pubmed] PHST- 2018/07/03 06:00 [medline] PHST- 2017/10/28 06:00 [entrez] AID - 10.1007/s00421-017-3746-2 [pii] AID - 10.1007/s00421-017-3746-2 [doi] PST - ppublish SO - Eur J Appl Physiol. 2017 Dec;117(12):2587-2600. doi: 10.1007/s00421-017-3746-2. Epub 2017 Oct 26.