PMID- 29079788
OWN - NLM
STAT- MEDLINE
DCOM- 20190806
LR  - 20190916
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 7
IP  - 1
DP  - 2017 Oct 27
TI  - Depletion of Mageb16 induces differentiation of pluripotent stem cells 
      predominantly into mesodermal derivatives.
PG  - 14285
LID - 10.1038/s41598-017-14561-z [doi]
LID - 14285
AB  - The Melanoma-associated Antigen gene family (MAGE) generally encodes for tumour 
      antigens. We had identified that one of the MAGE gene members, Mageb16 was highly 
      expressed in undifferentiated murine embryonic stem cells (ESCs). While the role 
      of Mageb16 in stemness and differentiation of pluripotent stem cells is 
      completely unknown, here, in our current study, we have demonstrated that Mageb16 
      (41 kDa) is distributed in cytosol and/or in surface membrane in undifferentiated 
      ESCs. A transcriptome study performed at  differentiated short hairpin RNA 
      (shRNA)-mediated Mageb16 knockdown (KD) ESCs and scrambled control (SCR) ESCs 
      until a period of 22 days, revealed that Mageb16 KD ESCs mainly differentiated 
      towards cells expressing mesodermal and cardiovascular lineage - gene markers. 
      Gene markers of other mesoderm-oriented biological processes such as 
      adipogenesis, osteogenesis, limb morphogenesis and spermatogenesis were also 
      significantly enriched in the differentiated Mageb16 KD ESCs. The expression 
      levels of contractile genes were higher in differentiated Mageb16 KD ESCs when 
      compared to differentiated SCR and wild ESCs, suggesting a higher cardiomyogenic 
      potential of Mageb16 depleted ESCs. Further analysis indicates  that regulative 
      epigenetic networks and nucleocytoplasmic modifications induced by the depletion 
      of Mageb16, may play a probable role in differentiation.
FAU - Gaspar, John Antonydas
AU  - Gaspar JA
AD  - University of Cologne (UKK), Institute of Neurophysiology and Center for 
      Molecular Medicine Cologne (CMMC), Robert-Koch-Str. 39, 50931, Cologne, Germany.
FAU - Srinivasan, Sureshkumar Perumal
AU  - Srinivasan SP
FAU - Sureshkumar, Poornima
AU  - Sureshkumar P
AD  - University of Cologne (UKK), Institute of Neurophysiology and Center for 
      Molecular Medicine Cologne (CMMC), Robert-Koch-Str. 39, 50931, Cologne, Germany.
FAU - Doss, Michael Xavier
AU  - Doss MX
AD  - University of Cologne (UKK), Institute of Neurophysiology and Center for 
      Molecular Medicine Cologne (CMMC), Robert-Koch-Str. 39, 50931, Cologne, Germany.
FAU - Hescheler, Jurgen
AU  - Hescheler J
AD  - University of Cologne (UKK), Institute of Neurophysiology and Center for 
      Molecular Medicine Cologne (CMMC), Robert-Koch-Str. 39, 50931, Cologne, Germany.
FAU - Papadopoulos, Symeon
AU  - Papadopoulos S
AD  - University of Cologne, Center of Physiology and Pathophysiology, Institute of 
      Vegetative Physiology, Robert-Koch-Str. 39, 50931, Cologne, Germany.
FAU - Sachinidis, Agapios
AU  - Sachinidis A
AD  - University of Cologne (UKK), Institute of Neurophysiology and Center for 
      Molecular Medicine Cologne (CMMC), Robert-Koch-Str. 39, 50931, Cologne, Germany. 
      a.sachinidis@uni-koeln.de.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171027
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Mageb16 protein, mouse)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (RNA, Small Interfering)
MH  - Animals
MH  - Antigens, Neoplasm/genetics
MH  - Cell Differentiation/*physiology
MH  - Cell Membrane/metabolism
MH  - Cells, Cultured
MH  - Cytosol/metabolism
MH  - Gene Expression
MH  - Gene Knockdown Techniques
MH  - Mice
MH  - Neoplasm Proteins/*deficiency/genetics
MH  - Pluripotent Stem Cells/cytology/*metabolism
MH  - RNA, Small Interfering/metabolism
MH  - Transcriptome
PMC - PMC5660239
COIS- The authors declare that they have no competing interests.
EDAT- 2017/10/29 06:00
MHDA- 2019/08/07 06:00
PMCR- 2017/10/27
CRDT- 2017/10/29 06:00
PHST- 2017/07/31 00:00 [received]
PHST- 2017/10/11 00:00 [accepted]
PHST- 2017/10/29 06:00 [entrez]
PHST- 2017/10/29 06:00 [pubmed]
PHST- 2019/08/07 06:00 [medline]
PHST- 2017/10/27 00:00 [pmc-release]
AID - 10.1038/s41598-017-14561-z [pii]
AID - 14561 [pii]
AID - 10.1038/s41598-017-14561-z [doi]
PST - epublish
SO  - Sci Rep. 2017 Oct 27;7(1):14285. doi: 10.1038/s41598-017-14561-z.