PMID- 29082265
OWN - NLM
STAT- MEDLINE
DCOM- 20180718
LR  - 20181113
IS  - 2314-7156 (Electronic)
IS  - 2314-8861 (Print)
IS  - 2314-7156 (Linking)
VI  - 2017
DP  - 2017
TI  - Vaccinomics Approach for Designing Potential Peptide Vaccine by Targeting 
      Shigella spp. Serine Protease Autotransporter Subfamily Protein SigA.
PG  - 6412353
LID - 10.1155/2017/6412353 [doi]
LID - 6412353
AB  - Shigellosis, a bacillary dysentery, is closely associated with diarrhoea in human 
      and causes infection of 165 million people worldwide per year. Casein-degrading 
      serine protease autotransporter of enterobacteriaceae (SPATE) subfamily protein 
      SigA, an outer membrane protein, exerts both cytopathic and enterotoxic effects 
      especially cytopathic to human epithelial cell type-2 (HEp-2) and is shown to be 
      highly immunogenic. In the present study, we have tried to impose the vaccinomics 
      approach for designing a common peptide vaccine candidate against the immunogenic 
      SigA of Shigella spp. At first, 44 SigA proteins from different variants of S. 
      flexneri, S. dysenteriae, S. boydii, and S. sonnei were assessed to find the most 
      antigenic protein. We retrieved 12 peptides based on the highest score for human 
      leukocyte antigen (HLA) supertypes analysed by NetCTL. Initially, these peptides 
      were assessed for the affinity with MHC class I and class II alleles, and four 
      potential core epitopes VTARAGLGY, FHTVTVNTL, HTTWTLTGY, and IELAGTLTL were 
      selected. From these, FHTVTVNTL and IELAGTLTL peptides were shown to have 100% 
      conservancy. Finally, IELAGTLTL was shown to have the highest population coverage 
      (83.86%) among the whole world population. In vivo study of the proposed epitope 
      might contribute to the development of functional and unique widespread vaccine, 
      which might be an operative alleyway to thwart dysentery from the world.
FAU - Oany, Arafat Rahman
AU  - Oany AR
AUID- ORCID: 0000-0003-2228-457X
AD  - Department of Biotechnology and Genetic Engineering, Faculty of Life Science, 
      Mawlana Bhashani Science and Technology University, Tangail, Bangladesh.
FAU - Pervin, Tahmina
AU  - Pervin T
AD  - Biotechnology and Genetic Engineering Discipline, Life Science School, Khulna 
      University, Khulna, Bangladesh.
FAU - Mia, Mamun
AU  - Mia M
AD  - Department of Biotechnology and Genetic Engineering, Faculty of Life Science, 
      Mawlana Bhashani Science and Technology University, Tangail, Bangladesh.
FAU - Hossain, Motaher
AU  - Hossain M
AD  - Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, 
      Bangladesh.
FAU - Shahnaij, Mohammad
AU  - Shahnaij M
AD  - Enteric and Food Microbiology Laboratory, International Centre for Diarrhoeal 
      Disease Research Bangladesh (icddr,b), Dhaka, Bangladesh.
FAU - Mahmud, Shahin
AU  - Mahmud S
AD  - Department of Biotechnology and Genetic Engineering, Faculty of Life Science, 
      Mawlana Bhashani Science and Technology University, Tangail, Bangladesh.
FAU - Kibria, K M Kaderi
AU  - Kibria KMK
AUID- ORCID: 0000-0002-8741-2918
AD  - Department of Biotechnology and Genetic Engineering, Faculty of Life Science, 
      Mawlana Bhashani Science and Technology University, Tangail, Bangladesh.
LA  - eng
PT  - Journal Article
DEP - 20170907
PL  - Egypt
TA  - J Immunol Res
JT  - Journal of immunology research
JID - 101627166
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Bacterial Vaccines)
RN  - 0 (Caseins)
RN  - 0 (HLA Antigens)
RN  - 0 (Immunodominant Epitopes)
RN  - 0 (Type V Secretion Systems)
RN  - 0 (Vaccines, Subunit)
SB  - IM
MH  - Antigens, Bacterial/*immunology
MH  - Bacterial Vaccines/*genetics
MH  - Caseins/metabolism
MH  - Diarrhea
MH  - Dysentery, Bacillary/*immunology
MH  - Epithelial Cells/*physiology
MH  - Epitope Mapping
MH  - HLA Antigens/metabolism
MH  - Humans
MH  - Immunodominant Epitopes/*genetics/immunology
MH  - Mass Vaccination
MH  - Protein Binding
MH  - Protein Conformation
MH  - Shigella/*immunology
MH  - Type V Secretion Systems/*genetics/immunology
MH  - Vaccines, Subunit/*genetics
PMC - PMC5610819
EDAT- 2017/10/31 06:00
MHDA- 2018/07/19 06:00
PMCR- 2017/09/07
CRDT- 2017/10/31 06:00
PHST- 2017/03/18 00:00 [received]
PHST- 2017/06/28 00:00 [revised]
PHST- 2017/07/24 00:00 [accepted]
PHST- 2017/10/31 06:00 [entrez]
PHST- 2017/10/31 06:00 [pubmed]
PHST- 2018/07/19 06:00 [medline]
PHST- 2017/09/07 00:00 [pmc-release]
AID - 10.1155/2017/6412353 [doi]
PST - ppublish
SO  - J Immunol Res. 2017;2017:6412353. doi: 10.1155/2017/6412353. Epub 2017 Sep 7.