PMID- 29082371
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 2476-4108 (Print)
IS  - 2476-3772 (Electronic)
IS  - 2476-3772 (Linking)
VI  - 15
IP  - 8
DP  - 2017 Aug
TI  - Frequency of TNFR1 36 A/G gene polymorphism in azoospermic infertile men: A 
      case-control study.
PG  - 521-526
AB  - BACKGROUND: Tumor necrosis factor-alpha (TNF-alpha) is a multifunctional cytokine 
      that regulates different cellular activities related to spermatogenesis. Tumor 
      necrosis factor-alpha receptor 1 (TNFR1) mediates TNF-alpha activity and polymorphism 
      in TNFR1 could lead to gene dysfunction and male infertility. OBJECTIVE: The aim 
      of this study is to determine the association of TNFR1 36 A/G polymorphism with 
      the idiopathic azoospermia in Iranian population. MATERIALS AND METHODS: This 
      case-control study included 108 azoospermic and 119 fertile men. This research 
      investigated the frequency of TNFR1 36 A/G polymorphism in cases who were 
      idiopathic azoospermic men referred to Yazd Research and Clinical Center for 
      Infertility, Iran in comparison with controls. polymerase chain reaction- 
      restriction fragment length polymorphism (PCR-RFLP) method was used to 
      investigate the polymorphism in both case and control groups. PCR fragments were 
      digested by Mspa1I enzyme and products were appeared by gel electrophoresis. The 
      abundance of A-->G was calculated in the azoospermic and healthy men. RESULTS: 
      According to the present study, GG and AG genotypes frequency in the azoospermic 
      men group were higher than the control group (OR= 2.298 (1.248-4.229), p=0.007), 
      (OR=1.47 (0.869-2.498, p=0.149). Our findings also showed that G allele frequency 
      in azoospermic men had significant difference compared to the control group 
      (OR=2.302 (1.580-3.355), p<0.001). CONCLUSION: It seems that the GG genotype and 
      G allele have an association with increased risk of non-obstructive azoospermia.
FAU - Ashrafzadeh, Hamid Reza
AU  - Ashrafzadeh HR
AD  - Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, 
      Ashkezar, Yazd, Iran.
FAU - Nazari, Tahere
AU  - Nazari T
AD  - Department of Medical Genetics, Tehran University of Medical Sciences, Tehran, 
      Iran.
FAU - Dehghan Tezerjani, Masoud
AU  - Dehghan Tezerjani M
AD  - Research and Clinical Center for Infertility, Yazd Reproductive Sciences 
      Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
FAU - Khademi Bami, Maryam
AU  - Khademi Bami M
AD  - Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, 
      Ashkezar, Yazd, Iran.
FAU - Ghasemi-Esmailabad, Saeed
AU  - Ghasemi-Esmailabad S
AD  - Recurrent Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid 
      Sadoughi University of Medical Sciences, Yazd, Iran.
FAU - Ghasemi, Nasrin
AU  - Ghasemi N
AD  - Recurrent Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid 
      Sadoughi University of Medical Sciences, Yazd, Iran.
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Int J Reprod Biomed
JT  - International journal of reproductive biomedicine
JID - 101679102
PMC - PMC5653914
OTO - NOTNLM
OT  - Cytokines
OT  - Male infertility
OT  - Polymorphism
OT  - Spermatozoa
OT  - Tumor necrosis factor alpha
COIS- The authors declare that there is no conflict of interest regarding this study.
EDAT- 2017/10/31 06:00
MHDA- 2017/10/31 06:01
PMCR- 2017/08/01
CRDT- 2017/10/31 06:00
PHST- 2017/10/31 06:00 [entrez]
PHST- 2017/10/31 06:00 [pubmed]
PHST- 2017/10/31 06:01 [medline]
PHST- 2017/08/01 00:00 [pmc-release]
AID - ijrb-15-521 [pii]
PST - ppublish
SO  - Int J Reprod Biomed. 2017 Aug;15(8):521-526.