PMID- 29082888
OWN - NLM
STAT- MEDLINE
DCOM- 20180906
LR  - 20211204
IS  - 2148-5607 (Electronic)
IS  - 1300-4948 (Print)
IS  - 1300-4948 (Linking)
VI  - 29
IP  - 1
DP  - 2018 Jan
TI  - Evaluation of mTOR signaling pathway proteins in rat gastric mucosa exposed to 
      sulfite and ghrelin.
PG  - 94-100
LID - 10.5152/tjg.2017.17294 [doi]
AB  - BACKGROUND/AIMS: Mammalian target of rapamycin (mTOR) signaling serves as a 
      central regulator of cell growth, proliferation, and survival. In this study, we 
      planned to evaluate the expressions of mTOR signaling constituents (p-p70S6K, 
      p-mTOR, and p-Tuberin) in rat gastric mucosa and to compare the results in 
      sulfite- and sulfite+ghrelin-exposed groups. MATERIALS AND METHODS: Rats were 
      divided into three groups: the control group (C), the sodium metabisulfite 
      (Na2S2O5) (S) group, and sulfite+ghrelin (SG) group. Sodium metabisulfite at 100 
      mg/kg/day was administered via gavage, and ghrelin at 20 mug/kg/day was 
      administered intraperitoneally for 35 days. We have used immunohistochemistry for 
      mTOR signaling pathway components. RESULTS: There were no significant differences 
      for p-p70S6K and p-mTOR expression among the C, S, and SG groups. Tuberin 
      expression was significantly increased in the S group compared to the C group. 
      Furthermore, tuberin expression was found to be significantly decreased in the SG 
      group. CONCLUSION: This study is the first one in the literature that shows the 
      expression of mTOR signaling proteins in gastric mucosa of rats exposed to 
      sulfite and ghrelin. Furthermore, it demonstrates that ghrelin treatment reduces 
      p-Tuberin expression induced by ingested sulfite.
FAU - Ercan, Sevim
AU  - Ercan S
AD  - Department of Medical Services and Techniques, Akdeniz University Vocational 
      School of Health Services, Antalya, Turkey.
FAU - Sahin, Pinar
AU  - Sahin P
AD  - Department of Histology and Embryology, Akdeniz University School of Medicine, 
      Antalya, Turkey.
FAU - Kencebay, Ceren
AU  - Kencebay C
AD  - Department of Biophysics, Akdeniz University School of Medicine, Antalya, Turkey.
FAU - Derin, Narin
AU  - Derin N
AD  - Department of Biophysics, Akdeniz University School of Medicine, Antalya, Turkey.
FAU - Celik Ozenci, Ciler
AU  - Celik Ozenci C
AD  - Department of Histology and Embryology, Akdeniz University School of Medicine, 
      Antalya, Turkey.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171030
PL  - Turkey
TA  - Turk J Gastroenterol
JT  - The Turkish journal of gastroenterology : the official journal of Turkish Society 
      of Gastroenterology
JID - 9515841
RN  - 0 (Ghrelin)
RN  - 0 (Sulfites)
RN  - 0 (Tuberous Sclerosis Complex 2 Protein)
RN  - 0 (Tumor Suppressor Proteins)
RN  - 4VON5FNS3C (sodium metabisulfite)
RN  - EC 2.7.1.1 (mTOR protein, rat)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 70-kDa)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Gastric Mucosa/drug effects/*metabolism
MH  - Ghrelin/*pharmacology
MH  - Male
MH  - Phosphorylation
MH  - Rats
MH  - Rats, Wistar
MH  - Ribosomal Protein S6 Kinases, 70-kDa/*metabolism
MH  - Signal Transduction
MH  - Sulfites/*pharmacology
MH  - TOR Serine-Threonine Kinases/*metabolism
MH  - Tuberous Sclerosis Complex 2 Protein
MH  - Tumor Suppressor Proteins/*metabolism
PMC - PMC6322605
COIS- Conflict of Interest: No conflict of interest was declared by the authors.
EDAT- 2017/10/31 06:00
MHDA- 2018/09/07 06:00
PMCR- 2018/01/01
CRDT- 2017/10/31 06:00
PHST- 2017/10/31 06:00 [pubmed]
PHST- 2018/09/07 06:00 [medline]
PHST- 2017/10/31 06:00 [entrez]
PHST- 2018/01/01 00:00 [pmc-release]
AID - tjg-29-1-94 [pii]
AID - 10.5152/tjg.2017.17294 [doi]
PST - ppublish
SO  - Turk J Gastroenterol. 2018 Jan;29(1):94-100. doi: 10.5152/tjg.2017.17294. Epub 
      2017 Oct 30.