PMID- 29084133
OWN - NLM
STAT- MEDLINE
DCOM- 20180608
LR  - 20211204
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 18
IP  - 11
DP  - 2017 Oct 30
TI  - Modulation of the Senescence-Associated Inflammatory Phenotype in Human 
      Fibroblasts by Olive Phenols.
LID - 10.3390/ijms18112275 [doi]
LID - 2275
AB  - Senescent cells display an increase in the secretion of growth factors, 
      inflammatory cytokines and proteolytic enzymes, termed the 
      "senescence-associated-secretory-phenotype" (SASP), playing a major role in many 
      age-related diseases. The phenolic compounds present in extra-virgin olive oil 
      are inhibitors of oxidative damage and have been reported to play a protective 
      role in inflammation-related diseases. Particularly, hydroxytyrosol and 
      oleuropein are the most abundant and more extensively studied. Pre-senescent 
      human lung (MRC5) and neonatal human dermal (NHDF) fibroblasts were used as 
      cellular model to evaluate the effect of chronic (4-6 weeks) treatment with 1 muM 
      hydroxytyrosol (HT) or 10 muM oleuropein aglycone (OLE) on senescence/inflammation 
      markers. Both phenols were effective in reducing beta-galactosidase-positive cell 
      number and p16 protein expression. In addition, senescence/inflammation markers 
      such as IL-6 and metalloprotease secretion, and Ciclooxigenase type 2 (COX-2) and 
      alpha-smooth-actin levels were reduced by phenol treatments. In NHDF, COX-2 
      expression, Nuclear Factor kappa-light-chain-enhancer of activated B cells (NFkappaB) 
      protein level and nuclear localization were augmented with culture senescence and 
      decreased by OLE and HT treatment. Furthermore, the inflammatory effect of Tumor 
      Necrosis Factor alpha (TNFalpha) exposure was almost completely abolished in OLE- and 
      HT-pre-treated NHDF. Thus, the modulation of the senescence-associated 
      inflammatory phenotype might be an important mechanism underlying the beneficial 
      effects of olive oil phenols.
FAU - Menicacci, Beatrice
AU  - Menicacci B
AD  - Department of Biomedical, Experimental and Clinical Science Mario Serio, 
      Experimental Pathology and Oncology Section, University of Florence, Viale 
      Morgagni 50, 50134 Florence, Italy. beatrice.menicacci@stud.unifi.it.
AD  - Department of Medical Biotechnologies, University of Siena, 53100 Siena, Italy. 
      beatrice.menicacci@stud.unifi.it.
FAU - Cipriani, Caterina
AU  - Cipriani C
AD  - NEUROFARBA Department, Pharmacology and Toxicology Section, University of 
      Florence, Viale Pieraccini 6, 50139 Florence, Italy. caterinacipriani8@gmail.com.
FAU - Margheri, Francesca
AU  - Margheri F
AD  - Department of Biomedical, Experimental and Clinical Science Mario Serio, 
      Experimental Pathology and Oncology Section, University of Florence, Viale 
      Morgagni 50, 50134 Florence, Italy. fmargheri@unifi.it.
FAU - Mocali, Alessandra
AU  - Mocali A
AUID- ORCID: 0000-0002-5673-1780
AD  - Department of Biomedical, Experimental and Clinical Science Mario Serio, 
      Experimental Pathology and Oncology Section, University of Florence, Viale 
      Morgagni 50, 50134 Florence, Italy. alessandra.mocali@unifi.it.
FAU - Giovannelli, Lisa
AU  - Giovannelli L
AUID- ORCID: 0000-0002-4027-4693
AD  - NEUROFARBA Department, Pharmacology and Toxicology Section, University of 
      Florence, Viale Pieraccini 6, 50139 Florence, Italy. lisa.giovannelli@unifi.it.
LA  - eng
PT  - Journal Article
DEP - 20171030
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Biomarkers)
RN  - 0 (Iridoid Glucosides)
RN  - 0 (Iridoids)
RN  - 0 (Phenols)
RN  - 0 (Plant Extracts)
RN  - 2O4553545L (oleuropein)
SB  - IM
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Biomarkers
MH  - Cell Line
MH  - Cells, Cultured
MH  - Cellular Senescence/*drug effects
MH  - Fibroblasts/*drug effects/*metabolism
MH  - Humans
MH  - Iridoid Glucosides
MH  - Iridoids/metabolism
MH  - Olea/*chemistry
MH  - Phenols/*pharmacology
MH  - Phenotype
MH  - Plant Extracts/*pharmacology
PMC - PMC5713245
OTO - NOTNLM
OT  - SASP
OT  - hydroxytyrosol
OT  - inflammatory phenotype
OT  - oleuropein
OT  - replicative senescence
COIS- The authors declare no conflict of interest.
EDAT- 2017/10/31 06:00
MHDA- 2018/06/09 06:00
PMCR- 2017/11/01
CRDT- 2017/10/31 06:00
PHST- 2017/09/27 00:00 [received]
PHST- 2017/10/19 00:00 [revised]
PHST- 2017/10/25 00:00 [accepted]
PHST- 2017/10/31 06:00 [entrez]
PHST- 2017/10/31 06:00 [pubmed]
PHST- 2018/06/09 06:00 [medline]
PHST- 2017/11/01 00:00 [pmc-release]
AID - ijms18112275 [pii]
AID - ijms-18-02275 [pii]
AID - 10.3390/ijms18112275 [doi]
PST - epublish
SO  - Int J Mol Sci. 2017 Oct 30;18(11):2275. doi: 10.3390/ijms18112275.