PMID- 29084628 OWN - NLM STAT- MEDLINE DCOM- 20180619 LR - 20180620 IS - 1873-6823 (Electronic) IS - 0741-8329 (Linking) VI - 65 DP - 2017 Dec TI - First description and evaluation of SNPs in the ADH and ALDH genes in a population of alcoholics in Central-West Brazil. PG - 37-43 LID - S0741-8329(16)30204-X [pii] LID - 10.1016/j.alcohol.2017.04.006 [doi] AB - Worldwide, different studies have reported an association of alcohol-use disorder (AUD) with different types of Single Nucleotide Polymorphisms (SNPs) in the genes for aldehyde dehydrogenase (ALDH) and alcohol dehydrogenase (ADH). In Brazil, there is little information about the occurrence of these SNPs in the AUD population and an absence of studies characterizing the population in the Central-West Region of Brazil. Actually, in Brazil, there are more than 4 million people with AUD. Despite the major health hazards of AUD, information on alcohol consumption and its consequences are not well understood. Therefore, it is extremely important to characterize these SNPs for the better understanding of AUD as a genetic disease in the Brazilian population. The present study, unlike other studies in other countries, is done with a subject population that shows a significant amount of racial homogenization. We evaluated the presence of SNPs in the ADH (ADH1B, ADH1C, and ADH4) and ALDH (ALDH2) genes in alcohol users of Goiania, State of Goias - Brazil, and then we established a possible relationship with AUD by allelic and genotypic study. This study was conducted with a population of people with AUD (n = 99) from Goias Alcohol Dependence Recovery Center (GO CEREA) and Psychosocial Care Center for Alcohol and Drugs (CAPS AD), and with a population of people without AUD as controls (n = 100). DNA was extracted from whole-blood samples and the genotyping was performed using TaqMan((R)) SNP genotyping assays. For characterization and evaluation of SNPs in the population, genotype frequency, allele frequency, haplotype frequency, Hardy-Weinberg equilibrium, and linkage disequilibrium were analyzed. Statistical analyses were calculated by GENEPOP 4.5 and Haploview software. The allele 1 was considered as "wild" (or *1) and allele 2 as mutant (or *2). Significant differences were found for ADH1B*, ADH4*2, and ALDH2*2 SNPs when the genotype and allele frequencies were analyzed. In addition, four haplotypes were observed between ADH1B*2 and ADH1C*2 through linkage disequilibrium analysis. The genetic variants may be associated with protection against AUD in the population studied. CI - Copyright (c) 2017 Elsevier Inc. All rights reserved. FAU - Teixeira, Thallita Monteiro AU - Teixeira TM AD - Laboratorio de Genetica Molecular e Citogenetica, Instituto de Ciencias Biologicas, Universidade Federal de Goias, Avenida Esperanca, s/n, Campus Samambaia (Campus II), Goiania, GO, CEP: 74690-900, Brazil. FAU - da Silva, Hugo Delleon AU - da Silva HD AD - Laboratorio de Genetica Molecular e Citogenetica, Instituto de Ciencias Biologicas, Universidade Federal de Goias, Avenida Esperanca, s/n, Campus Samambaia (Campus II), Goiania, GO, CEP: 74690-900, Brazil. FAU - Goveia, Rebeca Mota AU - Goveia RM AD - Laboratorio de Genetica Molecular e Citogenetica, Instituto de Ciencias Biologicas, Universidade Federal de Goias, Avenida Esperanca, s/n, Campus Samambaia (Campus II), Goiania, GO, CEP: 74690-900, Brazil. FAU - Ribolla, Paulo Eduardo Martins AU - Ribolla PEM AD - Laboratorio de Pesquisa e Analises Geneticas, Instituto de Biociencias de Botucatu, Universidade Estadual Paulista, Botucatu, Sao Paulo, Brazil. FAU - Alonso, Diego Peres AU - Alonso DP AD - Laboratorio de Pesquisa e Analises Geneticas, Instituto de Biociencias de Botucatu, Universidade Estadual Paulista, Botucatu, Sao Paulo, Brazil. FAU - Alves, Alessandro Arruda AU - Alves AA AD - Laboratorio de Mutagenese, Instituto de Ciencias Biologicas, Universidade Federal de Goias, Goiania, Brazil. FAU - Melo E Silva, Daniela AU - Melo E Silva D AD - Laboratorio de Mutagenese, Instituto de Ciencias Biologicas, Universidade Federal de Goias, Goiania, Brazil. FAU - Collevatti, Rosane Garcia AU - Collevatti RG AD - Laboratorio de Genetica e Biodiversidade, Instituto de Ciencias Biologicas, Universidade Federal de Goias, Goiania, Brazil. FAU - Bicudo, Lucilene Arilho AU - Bicudo LA AD - Laboratorio de Genetica Molecular e Citogenetica, Instituto de Ciencias Biologicas, Universidade Federal de Goias, Avenida Esperanca, s/n, Campus Samambaia (Campus II), Goiania, GO, CEP: 74690-900, Brazil. FAU - Bergamo, Nadia Aparecida AU - Bergamo NA AD - Laboratorio de Genetica Molecular e Citogenetica, Instituto de Ciencias Biologicas, Universidade Federal de Goias, Avenida Esperanca, s/n, Campus Samambaia (Campus II), Goiania, GO, CEP: 74690-900, Brazil. FAU - de Paula Silveira-Lacerda, Elisangela AU - de Paula Silveira-Lacerda E AD - Laboratorio de Genetica Molecular e Citogenetica, Instituto de Ciencias Biologicas, Universidade Federal de Goias, Avenida Esperanca, s/n, Campus Samambaia (Campus II), Goiania, GO, CEP: 74690-900, Brazil. Electronic address: elacerda@ufg.br. LA - eng PT - Journal Article DEP - 20170923 PL - United States TA - Alcohol JT - Alcohol (Fayetteville, N.Y.) JID - 8502311 RN - EC 1.1.1.1 (ADH1B protein, human) RN - EC 1.1.1.1 (ADH1C protein, human) RN - EC 1.1.1.1 (Alcohol Dehydrogenase) RN - EC 1.1.1.1 (alcohol dehydrogenase IV) RN - EC 1.2.1.3 (ALDH2 protein, human) RN - EC 1.2.1.3 (Aldehyde Dehydrogenase) RN - EC 1.2.1.3 (Aldehyde Dehydrogenase, Mitochondrial) SB - IM MH - Alcohol Dehydrogenase/*genetics MH - *Alcoholics MH - Alcoholism/diagnosis/epidemiology/*genetics MH - Aldehyde Dehydrogenase/genetics MH - Aldehyde Dehydrogenase, Mitochondrial/*genetics MH - Brazil/epidemiology MH - Case-Control Studies MH - Female MH - Humans MH - Male MH - Polymorphism, Single Nucleotide MH - Population Surveillance OTO - NOTNLM OT - ADH OT - ALDH OT - Alcohol-use disorder OT - Linkage disequilibrium OT - Polymorphism EDAT- 2017/11/01 06:00 MHDA- 2018/06/21 06:00 CRDT- 2017/11/01 06:00 PHST- 2016/09/29 00:00 [received] PHST- 2017/04/07 00:00 [revised] PHST- 2017/04/11 00:00 [accepted] PHST- 2017/11/01 06:00 [pubmed] PHST- 2018/06/21 06:00 [medline] PHST- 2017/11/01 06:00 [entrez] AID - S0741-8329(16)30204-X [pii] AID - 10.1016/j.alcohol.2017.04.006 [doi] PST - ppublish SO - Alcohol. 2017 Dec;65:37-43. doi: 10.1016/j.alcohol.2017.04.006. Epub 2017 Sep 23.