PMID- 29085079 OWN - NLM STAT- MEDLINE DCOM- 20190730 LR - 20221207 IS - 2045-2322 (Electronic) IS - 2045-2322 (Linking) VI - 7 IP - 1 DP - 2017 Oct 30 TI - Asymmetric Sensory-Motor Regeneration of Transected Peripheral Nerves Using Molecular Guidance Cues. PG - 14323 LID - 10.1038/s41598-017-14331-x [doi] LID - 14323 AB - Neural interfaces are designed to decode motor intent and evoke sensory precepts in amputees. In peripheral nerves, recording movement intent is challenging because motor axons are only a small fraction compared to sensory fibers and are heterogeneously mixed particularly at proximal levels. We previously reported that pain and myelinated axons regenerating through a Y-shaped nerve guide with sealed ends, can be modulated by luminar release of nerve growth factor (NGF) and neurotrophin-3 (NT-3), respectively. Here, we evaluate the differential potency of NGF, glial cell line-derived neurotrophic factor (GDNF), brain-derived neurotrophic factor (BDNF), pleiotrophin (PTN), and NT-3 in asymmetrically guiding the regeneration of sensory and motor neurons. We report that, in the absence of distal target organs, molecular guidance cues can mediate the growth of electrically conductive fascicles with normal microanatomy. Compared to Y-tube compartments with bovine serum albumin (BSA), GDNF and NGF increased the motor and sensory axon content, respectively. In addition, the sensory to motor ratio was significantly increased by PTN (12.7:1) when compared to a BDNF + GDNF choice. The differential content of motor and sensory axons modulated by selective guidance cues may provide a strategy to better define axon types in peripheral nerve interfaces. FAU - Anand, Sanjay AU - Anand S AD - Department of Bioengineering, University of Texas at Dallas, 800 W. Campbell Road, Richardson, TX, 75080, USA. FAU - Desai, Vidhi AU - Desai V AD - Department of Bioengineering, University of Texas at Arlington, 500 UTA Blvd, Arlington, Texas, 76010, USA. FAU - Alsmadi, Nesreen AU - Alsmadi N AD - Department of Bioengineering, University of Texas at Dallas, 800 W. Campbell Road, Richardson, TX, 75080, USA. FAU - Kanneganti, Aswini AU - Kanneganti A AD - Department of Bioengineering, University of Texas at Dallas, 800 W. Campbell Road, Richardson, TX, 75080, USA. FAU - Nguyen, Dianna Huyen-Tram AU - Nguyen DH AD - Department of Bioengineering, University of Texas at Arlington, 500 UTA Blvd, Arlington, Texas, 76010, USA. FAU - Tran, Martin AU - Tran M AD - Department of Bioengineering, University of Texas at Arlington, 500 UTA Blvd, Arlington, Texas, 76010, USA. FAU - Patil, Lokesh AU - Patil L AD - Department of Bioengineering, University of Texas at Dallas, 800 W. Campbell Road, Richardson, TX, 75080, USA. FAU - Vasudevan, Srikanth AU - Vasudevan S AD - Division of Biomedical Physics, Office of Science and Engineering Laboratories, Center for Devices and Radiological Health, U.S. Food and Drug Administration, Silver Spring, MD, 20993, USA. FAU - Xu, Cancan AU - Xu C AD - Department of Bioengineering, University of Texas at Arlington, 500 UTA Blvd, Arlington, Texas, 76010, USA. FAU - Hong, Yi AU - Hong Y AD - Department of Bioengineering, University of Texas at Arlington, 500 UTA Blvd, Arlington, Texas, 76010, USA. FAU - Cheng, Jonathan AU - Cheng J AD - Department of Plastic Surgery, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX, 75390, USA. FAU - Keefer, Edward AU - Keefer E AD - Nerves Incorporated, P.O. Box 141295, Dallas, TX 75214, USA. FAU - Romero-Ortega, Mario I AU - Romero-Ortega MI AD - Department of Bioengineering, University of Texas at Dallas, 800 W. Campbell Road, Richardson, TX, 75080, USA. Mario.Romero-Ortega@utdallas.edu. LA - eng GR - R56 NS095046/NS/NINDS NIH HHS/United States PT - Journal Article PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20171030 PL - England TA - Sci Rep JT - Scientific reports JID - 101563288 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Carrier Proteins) RN - 0 (Cytokines) RN - 0 (Glial Cell Line-Derived Neurotrophic Factor) RN - 0 (Neurotrophin 3) RN - 134034-50-7 (pleiotrophin) RN - 9061-61-4 (Nerve Growth Factor) MH - *Amputation, Surgical MH - Animals MH - Brain-Derived Neurotrophic Factor/*metabolism MH - Carrier Proteins/metabolism MH - Cells, Cultured MH - Cues MH - Cytokines/metabolism MH - Evoked Potentials, Motor/drug effects MH - Glial Cell Line-Derived Neurotrophic Factor/*metabolism MH - Mice MH - Motor Neurons/*physiology MH - Nerve Growth Factor/metabolism MH - *Nerve Regeneration MH - Neurotrophin 3/metabolism MH - Peripheral Nerves/*physiology MH - Sensory Receptor Cells/*physiology PMC - PMC5662603 COIS- The authors declare that they have no competing interests. EDAT- 2017/11/01 06:00 MHDA- 2019/07/31 06:00 PMCR- 2017/10/30 CRDT- 2017/11/01 06:00 PHST- 2017/07/07 00:00 [received] PHST- 2017/10/06 00:00 [accepted] PHST- 2017/11/01 06:00 [entrez] PHST- 2017/11/01 06:00 [pubmed] PHST- 2019/07/31 06:00 [medline] PHST- 2017/10/30 00:00 [pmc-release] AID - 10.1038/s41598-017-14331-x [pii] AID - 14331 [pii] AID - 10.1038/s41598-017-14331-x [doi] PST - epublish SO - Sci Rep. 2017 Oct 30;7(1):14323. doi: 10.1038/s41598-017-14331-x.