PMID- 29085305
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1664-042X (Print)
IS  - 1664-042X (Electronic)
IS  - 1664-042X (Linking)
VI  - 8
DP  - 2017
TI  - Heat and Hypoxic Acclimation Increase Monocyte Heat Shock Protein 72 but Do Not 
      Attenuate Inflammation following Hypoxic Exercise.
PG  - 811
LID - 10.3389/fphys.2017.00811 [doi]
LID - 811
AB  - Acclimation to heat or hypoxic stress activates the heat shock response and 
      accumulation of cytoprotective heat shock proteins (HSPs). By inhibiting the 
      NF-kappaB pathway HSP72 can preserve epithelial function and reduce systemic 
      inflammation. The aim of this study was to determine the time course of mHSP72 
      accumulation during acclimation, and to assess intestinal barrier damage and 
      systemic inflammation following hypoxic exercise. Three groups completed 10 x 
      60-min acclimation sessions (50% normoxic VO(2)peak) in control (n = 7; 18 degrees C, 35% 
      RH), hypoxic (n = 7; F(i)O(2) = 0.14, 18 degrees C, 35% RH), or hot (n = 7; 40 degrees C, 25% RH) 
      conditions. Tumor necrosis factor-alpha (TNF-alpha), interleukin 6 (IL-6), interleukin 10 
      (IL-10), and intestinal fatty acid binding protein (I-FABP) were determined at 
      rest and following a cycling normoxic stress test (NST; ~2 weeks before 
      acclimation), pre-acclimation hypoxic stress test (HST1; F(i)O(2) = 0.14, both at 
      50% normoxic VO(2)peak; ~1 week before acclimation) and post-acclimation HST (48 
      h; HST2). Monocyte HSP72 (mHSP72) was determined before and after exercise on day 
      1, 3, 5, 6, and 10 of acclimation. Accumulation of basal mHSP72 was evident from 
      day 5 (p < 0.05) of heat acclimation and increased further on day 6 (p < 0.01), 
      and day 10 (p < 0.01). In contrast, basal mHSP72 was elevated on the final day of 
      hypoxic acclimation (p < 0.05). Following the NST, plasma TNF-alpha (-0.11 +/- 0.27 
      ng(.)mL(-1)), IL-6 (+0.62 +/- 0.67 ng(.)mL(-1)) IL-10 (+1.09 +/- 9.06 ng(.)mL(-1)) 
      and I-FABP (+37.6 +/- 112.8 pg(.)mL(-1)) exhibited minimal change. After HST1, IL-6 
      (+3.87 +/- 2.56 ng(.)mL(-1)), IL-10 (+26.15 +/- 26.06 ng(.)mL(-1)) and I-FABP (+183.7 
      +/- 182.1 pg(.)mL(-1)) were elevated (p < 0.01), whereas TNF-alpha was unaltered (+0.08 
      +/- 1.27; p > 0.05). A similar trend was observed after HST2, with IL-6 (+3.09 +/- 
      1.30 ng(.)mL(-1)), IL-10 (+23.22 +/- 21.67 ng(.)mL(-1)) and I-FABP (+145.9 +/-123.2 
      pg(.)mL(-1)) increased from rest. Heat acclimation induces mHSP72 accumulation 
      earlier and at a greater magnitude compared to matched work hypoxic acclimation, 
      however neither acclimation regime attenuated the systemic cytokine response or 
      intestinal damage following acute exercise in hypoxia.
FAU - Lee, Ben J
AU  - Lee BJ
AD  - Occupational Performance Research Group, Department of Sport and Exercise 
      Sciences, University of Chichester, Chichester, United Kingdom.
AD  - Centre for Applied Biological and Exercise Sciences, Coventry University, 
      Coventry, United Kingdom.
FAU - Thake, Charles D
AU  - Thake CD
AD  - Occupational Performance Research Group, Department of Sport and Exercise 
      Sciences, University of Chichester, Chichester, United Kingdom.
LA  - eng
PT  - Journal Article
DEP - 20171016
PL  - Switzerland
TA  - Front Physiol
JT  - Frontiers in physiology
JID - 101549006
PMC - PMC5650636
OTO - NOTNLM
OT  - HSP72
OT  - I-FABP
OT  - Plasma cytokines
OT  - acclimation
OT  - cross-tolerance
EDAT- 2017/11/01 06:00
MHDA- 2017/11/01 06:01
PMCR- 2017/10/16
CRDT- 2017/11/01 06:00
PHST- 2017/05/21 00:00 [received]
PHST- 2017/10/02 00:00 [accepted]
PHST- 2017/11/01 06:00 [entrez]
PHST- 2017/11/01 06:00 [pubmed]
PHST- 2017/11/01 06:01 [medline]
PHST- 2017/10/16 00:00 [pmc-release]
AID - 10.3389/fphys.2017.00811 [doi]
PST - epublish
SO  - Front Physiol. 2017 Oct 16;8:811. doi: 10.3389/fphys.2017.00811. eCollection 
      2017.