PMID- 29085582
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 2008-3866 (Print)
IS  - 2008-3874 (Electronic)
IS  - 2008-3866 (Linking)
VI  - 20
IP  - 8
DP  - 2017 Aug
TI  - Rapamycin protects testes against germ cell apoptosis and oxidative stress 
      induced by testicular ischemia-reperfusion.
PG  - 905-911
LID - 10.22038/IJBMS.2017.9112 [doi]
AB  - OBJECTIVES: Rapamycin is an immunosuppressant compound with a broad spectrum of 
      pharmaco-logical activities. In recent years, it has been used successfully to 
      decrease ischemia-reperfusion injury in several organ systems. The purpose of the 
      present study was to examine the effect of rapamycin on testicular 
      ischemia-reperfusion injury. MATERIALS AND METHODS: Seventy-two adult male Wistar 
      rats were divided into six groups: control (group1), sham-operated (Group2), T/D 
      + DMSO as vehicle group (group3), and groups 4-6; respectively received 0.5, 1, 
      and 1.5 mgkg(-1) of rapamycin, IP 30 min before detorsion. Ischemia was achieved 
      by twisting the right testis 720 degrees  clockwise for 1 hr. The right testis of 6 
      animals from each group were excised 4 hr after detorsion for the measurement of 
      lipid peroxidation, caspase-3, and antioxidant enzyme activities. 
      Histopathological changes and germ cell apoptosis were determined by measuring 
      mean of seminiferous tubules diameters (MSTD) and TUNEL test in right testis of 6 
      animals per group, 24 hr after detorsion. RESULTS: Testicular T/D caused 
      increases in the apoptosis, malondialdehyde (MDA), and caspase-3 levels and 
      decreases in the superoxide dismutase (SOD), catalase (CAT), and glutathione 
      peroxidase (GPx) activities in ipsilateral testis (P<0.001). The rats treated 
      with rapamycin had significant decreases in the MDA and caspase-3 levels and 
      significant increases in the SOD, CAT and GPx activities in ipsilateral testis 
      compared with the T/D group (P<0.001); germ cell apoptosis was decreased, and 
      MSTD was improved. CONCLUSION: Rapamycin administration during testicular torsion 
      decreased ischemia/reperfusion (I/R) cellular damage.
FAU - Ghasemnejad-Berenji, Morteza
AU  - Ghasemnejad-Berenji M
AD  - Department of Pharmacology, School of Medicine, Tehran University of Medical 
      Sciences, Tehran, Iran.
AD  - Experimental Medicine Research Center, Tehran University of Medical Sciences, 
      Tehran, Iran.
AD  - Department of Pharmacology, Faculty of Pharmacy, Urmia University of Medical 
      Sciences, Urmia, Iran.
FAU - Ghazi-Khansari, Mahmoud
AU  - Ghazi-Khansari M
AD  - Department of Pharmacology, School of Medicine, Tehran University of Medical 
      Sciences, Tehran, Iran.
FAU - Yazdani, Iraj
AU  - Yazdani I
AD  - Department of Pharmacology, School of Medicine, Tehran University of Medical 
      Sciences, Tehran, Iran.
AD  - Experimental Medicine Research Center, Tehran University of Medical Sciences, 
      Tehran, Iran.
FAU - Saravi, Seyed Soheil Saeedi
AU  - Saravi SSS
AD  - Department of Pharmacology, School of Medicine, Tehran University of Medical 
      Sciences, Tehran, Iran.
AD  - Experimental Medicine Research Center, Tehran University of Medical Sciences, 
      Tehran, Iran.
AD  - Department of Pharmacology, Faculty of Pharmacy, Guilan University of Medical 
      Sciences, Rasht, Iran.
FAU - Nobakht, Maliheh
AU  - Nobakht M
AD  - Department of Anatomy, School of Medicine, Iran University of Medical Sciences, 
      Tehran, Iran.
FAU - Abdollahi, Alireza
AU  - Abdollahi A
AD  - Department of Pathology, Cancer Institute, Tehran University of Medical Sciences, 
      Tehran, Iran.
FAU - Ansari, Javad Mohajer
AU  - Ansari JM
AD  - Department of Anatomy, School of Medicine, Iran University of Medical Sciences, 
      Tehran, Iran.
FAU - Ghasemnejad-Berenji, Hojjat
AU  - Ghasemnejad-Berenji H
AD  - Department of Pathology, Cancer Institute, Tehran University of Medical Sciences, 
      Tehran, Iran.
FAU - Pashapour, Sarvin
AU  - Pashapour S
AD  - Department of Anatomy and Reproductive Biology, School of Medicine, Shahid 
      Beheshti University of Medical Sciences, Tehran, Iran.
FAU - Dehpour, Ahmad Reza
AU  - Dehpour AR
AD  - Department of Pharmacology, School of Medicine, Tehran University of Medical 
      Sciences, Tehran, Iran.
AD  - Experimental Medicine Research Center, Tehran University of Medical Sciences, 
      Tehran, Iran.
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Iran J Basic Med Sci
JT  - Iranian journal of basic medical sciences
JID - 101517966
PMC - PMC5651476
OTO - NOTNLM
OT  - Apoptosis
OT  - Ischemia-reperfusion
OT  - Rapamycin
OT  - Testicular torsion
OT  - Testis
EDAT- 2017/11/01 06:00
MHDA- 2017/11/01 06:01
PMCR- 2017/08/01
CRDT- 2017/11/01 06:00
PHST- 2017/11/01 06:00 [entrez]
PHST- 2017/11/01 06:00 [pubmed]
PHST- 2017/11/01 06:01 [medline]
PHST- 2017/08/01 00:00 [pmc-release]
AID - IJBMS-20-905 [pii]
AID - 10.22038/IJBMS.2017.9112 [doi]
PST - ppublish
SO  - Iran J Basic Med Sci. 2017 Aug;20(8):905-911. doi: 10.22038/IJBMS.2017.9112.