PMID- 29086205
OWN - NLM
STAT- MEDLINE
DCOM- 20190114
LR  - 20240209
IS  - 1466-1268 (Electronic)
IS  - 1355-8145 (Print)
IS  - 1355-8145 (Linking)
VI  - 23
IP  - 3
DP  - 2018 May
TI  - Testicular torsion and reperfusion: evidences for biochemical and molecular 
      alterations.
PG  - 429-439
LID - 10.1007/s12192-017-0855-0 [doi]
AB  - This study was done in order to determine the molecular and biochemical 
      alterations following testicular torsion (TT) and torsion-reperfusion (TR). For 
      this purpose, 54 male Wistar rats were divided into five groups as control group 
      (n = 6) and experimental group subjected to 1, 2, 4, and 8 h unilateral left 
      torsion induction (n = 12 in each group). After induction of TT, testicular 
      samples were collected from each group (n = 6), and the other six rats of each 
      group underwent the same period of reperfusion after TT and then were sampled. 
      Histological changes, the mRNA and protein expression of heat shock protein-70 
      (Hsp70), and caspase-3 were examined using reverse transcriptase-PCR (RT-PCR) and 
      immunohistochemistry, respectively. Testicular total antioxidant capacity (TAC), 
      glutathione peroxidase (GSH-px), and malondialdehyde (MDA) levels were evaluated. 
      The mRNA damage and DNA fragmentation were assessed. The TT and TR significantly 
      reduced differentiation and spermiogenesis indices (p < 0.05). The TT- and 
      TR-induced groups exhibited a severe reduction in Hsp70 expression as well as 
      remarkable enhancement in caspase-3 expression. The TAC and GSH-px levels were 
      decreased and the MDA content was increased in TT- and TR-induced groups. 
      Finally, the TT and TR enhanced mRNA damage and DNA fragmentation. The TT- and 
      TR-induced damaging oxidative stress, diminished Hsp70 expression, and enhanced 
      caspase-3 mRNA and protein levels result in apoptosis following 1, 2, and 4 h. 
      Whereas, following 8 h, TT and TR initiate the necrosis by inducing energy 
      depletion as well as severe mRNA damage.
FAU - Shamsi-Gamchi, Naeimeh
AU  - Shamsi-Gamchi N
AD  - Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, 
      P.O. BOX: 1177, Urmia, Iran.
FAU - Razi, Mazdak
AU  - Razi M
AUID- ORCID: 0000-0002-4309-4831
AD  - Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, 
      P.O. BOX: 1177, Urmia, Iran. mazdak.razi@gmail.com.
FAU - Behfar, Mehdi
AU  - Behfar M
AD  - Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, 
      Urmia University, Urmia, Iran.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171031
PL  - Netherlands
TA  - Cell Stress Chaperones
JT  - Cell stress & chaperones
JID - 9610925
RN  - 0 (Antioxidants)
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (RNA, Messenger)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Animals
MH  - Antioxidants/metabolism
MH  - Caspase 3/metabolism
MH  - DNA Fragmentation
MH  - HSP70 Heat-Shock Proteins/genetics/metabolism
MH  - Male
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats, Wistar
MH  - Reperfusion Injury/*genetics/*pathology
MH  - Seminiferous Tubules/pathology
MH  - Spermatic Cord Torsion/*genetics/*pathology
MH  - Spermatogenesis/genetics
PMC - PMC5904087
OTO - NOTNLM
OT  - Apoptosis
OT  - Caspase-3
OT  - Hsp70
OT  - Necrosis
OT  - Rat
OT  - Torsion-reperfusion
EDAT- 2017/11/01 06:00
MHDA- 2019/01/15 06:00
PMCR- 2018/11/01
CRDT- 2017/11/01 06:00
PHST- 2017/05/31 00:00 [received]
PHST- 2017/10/18 00:00 [accepted]
PHST- 2017/09/19 00:00 [revised]
PHST- 2017/11/01 06:00 [pubmed]
PHST- 2019/01/15 06:00 [medline]
PHST- 2017/11/01 06:00 [entrez]
PHST- 2018/11/01 00:00 [pmc-release]
AID - S1355-8145(23)00302-4 [pii]
AID - 855 [pii]
AID - 10.1007/s12192-017-0855-0 [doi]
PST - ppublish
SO  - Cell Stress Chaperones. 2018 May;23(3):429-439. doi: 10.1007/s12192-017-0855-0. 
      Epub 2017 Oct 31.