PMID- 29086456
OWN - NLM
STAT- MEDLINE
DCOM- 20180801
LR  - 20180815
IS  - 1365-313X (Electronic)
IS  - 0960-7412 (Linking)
VI  - 93
IP  - 1
DP  - 2018 Jan
TI  - TAF15b, involved in the autonomous pathway for flowering, represses transcription 
      of FLOWERING LOCUS C.
PG  - 79-91
LID - 10.1111/tpj.13758 [doi]
AB  - TATA-binding protein-associated factors (TAFs) are general transcription factors 
      within the transcription factor IID (TFIID) complex, which recognizes the core 
      promoter of genes. In addition to their biochemical function, it is known that 
      several TAFs are involved in the regulation of developmental processes. In this 
      study, we found that TAF15b affects flowering time, especially through the 
      autonomous pathway (AP) in Arabidopsis. The mutant taf15b shows late flowering 
      compared with the wild type plant during both long and short days, and 
      vernalization accelerates the flowering time of taf15b. In addition, taf15b shows 
      strong upregulation of FLOWERING LOCUS C (FLC), a flowering repressor in 
      Arabidopsis, and the flc taf15b double mutant completely offsets the late 
      flowering of taf15b, indicating that TAF15b is a typical AP gene. The taf15b 
      mutant also shows increased transcript levels of COOLAIR, an antisense transcript 
      of FLC. Consistently, chromatin immunoprecipitation (ChIP) analyses showed that 
      the TAF15b protein is enriched around both sense and antisense transcription 
      start sites of the FLC locus. In addition, co-immunoprecipitation showed that 
      TAF15b interacts with RNA polymerase II (Pol II), while ChIP showed increased 
      enrichment of the phosphorylated forms, both serine 2 (Ser2) and Ser5, of the 
      C-terminal domain of Pol II at the FLC locus, which is indicative of 
      transcriptional elongation. Finally, taf15b showed higher enrichment of the 
      active histone marker, H3K4me3, on FLC chromatin. Taken together, our results 
      suggest that TAF15b affects flowering time through transcriptional repression of 
      FLC in Arabidopsis.
CI  - (c) 2017 The Authors The Plant Journal (c) 2017 John Wiley & Sons Ltd.
FAU - Eom, Hyunjoo
AU  - Eom H
AD  - School of Biological Sciences, Seoul National University, Seoul, 08826, Korea.
AD  - Center for RNA Research, Institute for Basic Science, Seoul, 08826, Korea.
FAU - Park, Su Jung
AU  - Park SJ
AD  - Department of Plant Biotechnology, College of Agriculture and Life Sciences, 
      Chonnam National University, Gwangju, 61186, Korea.
FAU - Kim, Min Kyung
AU  - Kim MK
AD  - Department of Plant Biotechnology, College of Agriculture and Life Sciences, 
      Chonnam National University, Gwangju, 61186, Korea.
FAU - Kim, Hoyeun
AU  - Kim H
AD  - School of Biological Sciences, Seoul National University, Seoul, 08826, Korea.
FAU - Kang, Hunseung
AU  - Kang H
AD  - Department of Plant Biotechnology, College of Agriculture and Life Sciences, 
      Chonnam National University, Gwangju, 61186, Korea.
FAU - Lee, Ilha
AU  - Lee I
AD  - School of Biological Sciences, Seoul National University, Seoul, 08826, Korea.
AD  - Plant Genomics and Breeding Institute, Seoul National University, Seoul, 08826, 
      Korea.
LA  - eng
SI  - GENBANK/GQ352646
SI  - GENBANK/GQ342259
SI  - GENBANK/NP_053733
SI  - GENBANK/NP_004951
SI  - GENBANK/NP_631961
PT  - Journal Article
DEP - 20171130
PL  - England
TA  - Plant J
JT  - The Plant journal : for cell and molecular biology
JID - 9207397
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Chromatin)
RN  - 0 (FLF protein, Arabidopsis)
RN  - 0 (Histones)
RN  - 0 (MADS Domain Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (TAF15b protein, Arabidopsis)
RN  - 0 (TATA-Binding Protein Associated Factors)
RN  - EC 2.7.7.- (RNA Polymerase II)
SB  - IM
MH  - Arabidopsis/*genetics/growth & development/physiology
MH  - Arabidopsis Proteins/genetics/*metabolism
MH  - Chromatin/genetics
MH  - Chromatin Immunoprecipitation
MH  - Flowers/genetics/growth & development/physiology
MH  - *Gene Expression Regulation, Plant
MH  - Histones/genetics
MH  - MADS Domain Proteins/genetics/*metabolism
MH  - Mutation
MH  - Promoter Regions, Genetic/genetics
MH  - RNA Polymerase II/genetics/metabolism
MH  - Repressor Proteins/genetics/metabolism
MH  - TATA-Binding Protein Associated Factors/genetics/*metabolism
MH  - Time Factors
MH  - Up-Regulation
OTO - NOTNLM
OT  - Arabidopsis thaliana
OT  - FLOWERING LOCUS C (FLC)
OT  - RNA polymerase II
OT  - TBP-associated factor 15b (TAF15b)
OT  - autonomous pathway
OT  - flowering time
OT  - transcriptional regulation
EDAT- 2017/11/01 06:00
MHDA- 2018/08/02 06:00
CRDT- 2017/11/01 06:00
PHST- 2017/08/17 00:00 [received]
PHST- 2017/09/26 00:00 [revised]
PHST- 2017/10/25 00:00 [accepted]
PHST- 2017/11/01 06:00 [pubmed]
PHST- 2018/08/02 06:00 [medline]
PHST- 2017/11/01 06:00 [entrez]
AID - 10.1111/tpj.13758 [doi]
PST - ppublish
SO  - Plant J. 2018 Jan;93(1):79-91. doi: 10.1111/tpj.13758. Epub 2017 Nov 30.