PMID- 29088760
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240327
IS  - 1949-2553 (Electronic)
IS  - 1949-2553 (Linking)
VI  - 8
IP  - 43
DP  - 2017 Sep 26
TI  - The shortening of leukocyte telomere length relates to DNA hypermethylation of 
      LINE-1 in type 2 diabetes mellitus.
PG  - 73964-73973
LID - 10.18632/oncotarget.18167 [doi]
AB  - BACKGROUND: We aim to investigate the cross-talking of leukocyte telomere length 
      (LTL) and DNA methylation of LINE-1 in type 2 diabetes mellitus (T2DM). RESULTS: 
      LTL (ratio of the copy number of telomere [T] repeats to that of a single [S] 
      gene) was significantly shortened in T2DM compared with controls (0.94 +/- 0.41 vs. 
      1.14 +/- 0.48, P < 0.001), and decreased steadily with age in both controls and 
      T2DM. Conversely, significant increase of LINE-1 DNA methylation was found in 
      T2DM compared with controls (49.60 +/- 14.55 vs. 37.81 +/- 9.07, P < 0.001). 
      Moreover, age, HbA1c, and LINE-1 methylation ratio were stably negatively related 
      with LTL after multi-adjustment. Shorter LTL was associated with an increased 
      risk of T2DM [adjusted OR (95% CI) = 2.458 (1.192, 5.070), P = 0.015], while 
      lower LINE-1 DNA methylation levels could reduce the risk of T2DM [adjusted OR 
      (95% CI) = 0.189 (0.089, 0.400), P < 0.001]. MATERIALS AND METHODS: We performed 
      a hospital-based case-control study of 205 T2DM patients and 213 subjects of 
      healthy control with sex and age matched. LTL and DNA methylation of LINE-1 was 
      measured by quantitative PCR and quantitative methylation-specific PCR (qMSP), 
      respectively. CONCLUSIONS: Our research demonstrates the association between 
      shorter LTL and LINE-1 hyper-methylation in Chinese T2DM patients. These findings 
      suggest that shorter LTL might be associated with T2DM in a manner dependent of 
      epigenetic level.
FAU - Wu, Yue
AU  - Wu Y
AD  - Department of Clinical Laboratory, National Cancer Center/Cancer Hospital, 
      Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 
      100021, China.
AD  - Department of Clinical Laboratory, Peking Union Medical College Hospital, Peking 
      Union Medical College and Chinese Academy of Medical Sciences, Beijing, 100730, 
      China.
FAU - Cui, Wei
AU  - Cui W
AD  - Department of Clinical Laboratory, National Cancer Center/Cancer Hospital, 
      Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 
      100021, China.
AD  - Department of Clinical Laboratory, Peking Union Medical College Hospital, Peking 
      Union Medical College and Chinese Academy of Medical Sciences, Beijing, 100730, 
      China.
FAU - Zhang, Donghong
AU  - Zhang D
AD  - Department of Cardiology, The Second Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou 325027, Zhejiang, China.
AD  - Department of Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, 
      USA.
FAU - Wu, Wei
AU  - Wu W
AD  - Department of Clinical Laboratory, Peking Union Medical College Hospital, Peking 
      Union Medical College and Chinese Academy of Medical Sciences, Beijing, 100730, 
      China.
FAU - Yang, Zhuo
AU  - Yang Z
AD  - Department of Clinical Laboratory, Peking Union Medical College Hospital, Peking 
      Union Medical College and Chinese Academy of Medical Sciences, Beijing, 100730, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20170522
PL  - United States
TA  - Oncotarget
JT  - Oncotarget
JID - 101532965
PMC - PMC5650315
OTO - NOTNLM
OT  - DNA methylation
OT  - LINE-1
OT  - glycated hemoglobin
OT  - leukocyte telomere length
OT  - type 2 diabetes mellitus
COIS- CONFLICTS OF INTEREST The authors declare no competing financial interests.
EDAT- 2017/11/02 06:00
MHDA- 2017/11/02 06:01
PMCR- 2017/09/26
CRDT- 2017/11/02 06:00
PHST- 2017/04/04 00:00 [received]
PHST- 2017/05/11 00:00 [accepted]
PHST- 2017/11/02 06:00 [entrez]
PHST- 2017/11/02 06:00 [pubmed]
PHST- 2017/11/02 06:01 [medline]
PHST- 2017/09/26 00:00 [pmc-release]
AID - 18167 [pii]
AID - 10.18632/oncotarget.18167 [doi]
PST - epublish
SO  - Oncotarget. 2017 May 22;8(43):73964-73973. doi: 10.18632/oncotarget.18167. 
      eCollection 2017 Sep 26.