PMID- 29089999 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20200930 IS - 1673-5374 (Print) IS - 1876-7958 (Electronic) IS - 1673-5374 (Linking) VI - 12 IP - 9 DP - 2017 Sep TI - Jisuikang, a Chinese herbal formula, increases neurotrophic factor expression and promotes the recovery of neurological function after spinal cord injury. PG - 1519-1528 LID - 10.4103/1673-5374.215264 [doi] AB - The Chinese medicine compound, Jisuikang, can promote recovery of neurological function by inhibiting lipid peroxidation, scavenging oxygen free radicals, and effectively improving the local microenvironment after spinal cord injury. However, the mechanism remains unclear. Thus, we established a rat model of acute spinal cord injury using a modified version of Allen's method. Jisuikang (50, 25, and 12.5 g/kg/d) and prednisolone were administered 30 minutes after anesthesia. Basso, Beattie, and Bresnahan locomotor scale scores and the oblique board test showed improved motor function recovery in the prednisone group and moderate-dose Jisuikang group compared with the other groups at 3-7 days post-injury. The rats in the moderate-dose Jisuikang group recovered best at 14 days post-injury. Hematoxylin-eosin staining and transmission electron microscopy showed that the survival rate of neurons in treatment groups increased after 3-7 days of administration. Further, the structure of neurons and glial cells was more distinct, especially in prednisolone and moderate-dose Jisuikang groups. Western blot assay and immunohistochemistry showed that expression of brain-derived neurotrophic factor (BDNF) in injured segments was maintained at a high level after 7-14 days of treatment. In contrast, expression of nerve growth factor (NGF) was down-regulated at 7 days after spinal cord injury. Real-time fluorescence quantitative polymerase chain reaction showed that expression of BDNF and NGF mRNA was induced in injured segments by prednisolone and Jisuikang. At 3-7 days after injury, the effect of prednisolone was greater, while 14 days after injury, the effect of moderate-dose Jisuikang was greater. These results confirm that Jisuikang can upregulate BDNF and NGF expression for a prolonged period after spinal cord injury and promote repair of acute spinal cord injury, with its effect being similar to prednisolone. FAU - Guo, Yang AU - Guo Y AD - Institute of Traumatology & Orthopedics and Laboratory of New Techniques of Restoration & Reconstruction of Orthopedics and Traumatology, Nanjing University of Chinese Medicine, Nanjing, Jiangsu Province, China. FAU - Ma, Yong AU - Ma Y AD - Institute of Traumatology & Orthopedics and Laboratory of New Techniques of Restoration & Reconstruction of Orthopedics and Traumatology, Nanjing University of Chinese Medicine, Nanjing, Jiangsu Province, China. AD - Department of Traumatology & Orthopedics, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu Province, China. FAU - Pan, Ya-Lan AU - Pan YL AD - Institute of Traumatology & Orthopedics and Laboratory of New Techniques of Restoration & Reconstruction of Orthopedics and Traumatology, Nanjing University of Chinese Medicine, Nanjing, Jiangsu Province, China. FAU - Zheng, Su-Yang AU - Zheng SY AD - Institute of Traumatology & Orthopedics and Laboratory of New Techniques of Restoration & Reconstruction of Orthopedics and Traumatology, Nanjing University of Chinese Medicine, Nanjing, Jiangsu Province, China. FAU - Wang, Jian-Wei AU - Wang JW AD - Department of Traumatology & Orthopedics, Wuxi Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Wuxi, Jiangsu Province, China. FAU - Huang, Gui-Cheng AU - Huang GC AUID- ORCID: 0000-0002-7518-4073 AD - Institute of Traumatology & Orthopedics and Laboratory of New Techniques of Restoration & Reconstruction of Orthopedics and Traumatology, Nanjing University of Chinese Medicine, Nanjing, Jiangsu Province, China. LA - eng PT - Journal Article PL - India TA - Neural Regen Res JT - Neural regeneration research JID - 101316351 PMC - PMC5649474 OTO - NOTNLM OT - Chinese medicine OT - Jisuikang OT - axon regeneration OT - brain-derived neurotrophic factor OT - microenvironment OT - nerve growth factor OT - nerve regeneration OT - neural regeneration OT - neuronal apoptosis OT - prednisolone OT - secondary changes OT - spinal cord injury COIS- Conflicts of interest: None declared. EDAT- 2017/11/02 06:00 MHDA- 2017/11/02 06:01 PMCR- 2017/09/01 CRDT- 2017/11/02 06:00 PHST- 2017/11/02 06:00 [entrez] PHST- 2017/11/02 06:00 [pubmed] PHST- 2017/11/02 06:01 [medline] PHST- 2017/09/01 00:00 [pmc-release] AID - NRR-12-1519 [pii] AID - 10.4103/1673-5374.215264 [doi] PST - ppublish SO - Neural Regen Res. 2017 Sep;12(9):1519-1528. doi: 10.4103/1673-5374.215264.