PMID- 29091303
OWN - NLM
STAT- MEDLINE
DCOM- 20190415
LR  - 20220408
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Linking)
VI  - 119
IP  - 7
DP  - 2018 Jul
TI  - MiR-613 suppressed the laryngeal squamous cell carcinoma progression through 
      regulating PDK1.
PG  - 5118-5125
LID - 10.1002/jcb.26468 [doi]
AB  - MicroRNAs (miRNAs) are aberrantly expressed in several tumors and play important 
      role in tumorigenesis. However, little is known about the role of miR-613 in 
      laryngeal squamous cell carcinoma (LSCC). We determined the expression of miR-613 
      in a panel of 30 LSCC specimens. Compared with the adjacent normal samples, 20 
      cases of LSCC tissues exhibited decreased expression of miR-613. The average 
      expression of miR-613 in LSCC tissues was lower than in normal samples. Moreover, 
      we demonstrated that exogenous expression of miR-613 suppressed LSCC cell 
      proliferation, invasion, and blocked G1/S phase transition. We identified that 
      3-phosphoinositide-dependent protein kinase-1 (PDK1) was a direct target gene of 
      miR-613 in LSCC cell. Overexpression of miR-613 suppressed PDK1 expression in 
      LSCC cell. Furthermore, we demonstrated that PDK1 was upregulated in LSCC 
      tissues. MiR-613 expression was inversely correlated with the expression of PDK1 
      in LSCC tissues. Moreover, we showed that PDK1 was involved in the 
      miR-613-mediated cancer suppression of LSCC cell. These results suggested that 
      miR-613 played as a tumor suppressor gene in LSCC partly by inhibiting PDK1 
      expression.
CI  - (c) 2017 Wiley Periodicals, Inc.
FAU - Wang, Jing
AU  - Wang J
AD  - Department of Otolaryngology, Liaocheng People's Hospital and EENT Hospital, 
      Liaocheng, Shandong, China.
FAU - Yang, Shujuan
AU  - Yang S
AD  - Department of Otolaryngology, Liaocheng People's Hospital and EENT Hospital, 
      Liaocheng, Shandong, China.
FAU - Ge, Wensheng
AU  - Ge W
AUID- ORCID: 0000-0003-2606-3504
AD  - Department of Otolaryngology, Liaocheng People's Hospital and EENT Hospital, 
      Liaocheng, Shandong, China.
FAU - Wang, Ying
AU  - Wang Y
AD  - Department of Otolaryngology, Liaocheng People's Hospital and EENT Hospital, 
      Liaocheng, Shandong, China.
FAU - Han, Chaodong
AU  - Han C
AD  - Department of Otolaryngology, Liaocheng People's Hospital and EENT Hospital, 
      Liaocheng, Shandong, China.
FAU - Li, Maocai
AU  - Li M
AD  - Department of Otolaryngology, Liaocheng People's Hospital and EENT Hospital, 
      Liaocheng, Shandong, China.
LA  - eng
PT  - Journal Article
DEP - 20180406
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (MIRN613 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (PDK1 protein, human)
RN  - 0 (Pyruvate Dehydrogenase Acetyl-Transferring Kinase)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
SB  - IM
MH  - Blotting, Western
MH  - Cell Cycle/genetics/physiology
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics/physiology
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics
MH  - MicroRNAs/genetics/*metabolism
MH  - Protein Serine-Threonine Kinases/genetics/*metabolism
MH  - Pyruvate Dehydrogenase Acetyl-Transferring Kinase
MH  - Real-Time Polymerase Chain Reaction
OTO - NOTNLM
OT  - PDK1
OT  - laryngeal squamous cell carcinoma
OT  - miR-613
OT  - miRNAs
EDAT- 2017/11/02 06:00
MHDA- 2019/04/16 06:00
CRDT- 2017/11/02 06:00
PHST- 2017/08/15 00:00 [received]
PHST- 2017/10/31 00:00 [accepted]
PHST- 2017/11/02 06:00 [pubmed]
PHST- 2019/04/16 06:00 [medline]
PHST- 2017/11/02 06:00 [entrez]
AID - 10.1002/jcb.26468 [doi]
PST - ppublish
SO  - J Cell Biochem. 2018 Jul;119(7):5118-5125. doi: 10.1002/jcb.26468. Epub 2018 Apr 
      6.