PMID- 29093800 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20200930 IS - 1993-0771 (Print) IS - 1993-078X (Electronic) IS - 1993-0771 (Linking) VI - 11 IP - 3 DP - 2017 TI - Diversification of the P genome among Agropyron Gaertn. (Poaceae) species detected by FISH. PG - 495-509 LID - 10.3897/CompCytogen.v11i3.13124 [doi] AB - The genomes of five Agropyron Gaertner, 1770 species were characterized using all potential di- or trinucleotide simple sequence repeat (SSR) motifs and four satellite DNA repeats as fluorescence in situ hybridization (FISH) probes. The sites of 5S and 45S rDNA were relatively conserved among the diploid and tetraploid species. A number of sites for the dinucleotide SSRs AC, AG, and pSc119.2 was detected in all investigated species except A. mongolicum Keng, 1938. Several different trinucleotide SSRs were identified in different tetraploid species. All Agropyron species were suggested to include the basic P genome, although genome differentiation was still observed. The P genome of A. mongolicum was distinct from that of the diploid A. cristatum (Linnaeus, 1753) Gaertner, 1770. and other tetraploid species, with no hybridizations for AC, AG, or pSc119.2 observed. This finding supports designation of the P genomes of A. cristatum and A. mongolicum as P(c) and P(m), respectively. An exceptional 5S rDNA site revealed in one set of homoeologous chromosomes strongly supports the allopolyploid origin of A. desertorum (Fischer ex Link, 1821) Schultes, 1824. However, the diploid donors to A. desertorum need further investigation. Similarly, the unique FISH pattern of a pair of 5S rDNA-carrying chromosomes was indicative of a potential allopolyploid origin for A. fragile (Roth, 1800) Candargy, 1984. The conserved distribution of 5S and 45S rDNA suggests A. cristatum (4x) and A. michnoi Roshevitz, 1929 are closely related. Two forms of B chromosomes were identified among individuals A. mongolicum and A. desertorum plants. FAU - Zhao, Yan AU - Zhao Y AD - College of Grassland, Resource and Environmental Science, Inner Mongolia Agricultural University, Hohhot, 010011, China. AD - Key Laboratory of Grassland Resources (IMAU), Ministry of Education, Hohhot, 010011, China. AD - Key Laboratory of Forage Cultivation, Processing and High Efficient Utilization of Ministry of Agriculture, Hohhot, 010011, China. FAU - Xie, Jihong AU - Xie J AD - Grassland Research Institute, Chinese Academy of Agricultural Sciences, Hohhot, 010010, China. FAU - Dou, Quanwen AU - Dou Q AD - Key Laboratory of Crop Molecular Breeding, Qinghai Province, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China. FAU - Wang, Junjie AU - Wang J AD - College of Grassland, Resource and Environmental Science, Inner Mongolia Agricultural University, Hohhot, 010011, China. AD - Key Laboratory of Grassland Resources (IMAU), Ministry of Education, Hohhot, 010011, China. AD - Key Laboratory of Forage Cultivation, Processing and High Efficient Utilization of Ministry of Agriculture, Hohhot, 010011, China. FAU - Zhang, Zhong AU - Zhang Z AD - College of Grassland, Resource and Environmental Science, Inner Mongolia Agricultural University, Hohhot, 010011, China. AD - Key Laboratory of Grassland Resources (IMAU), Ministry of Education, Hohhot, 010011, China. AD - Key Laboratory of Forage Cultivation, Processing and High Efficient Utilization of Ministry of Agriculture, Hohhot, 010011, China. LA - eng PT - Journal Article DEP - 20170803 PL - Bulgaria TA - Comp Cytogenet JT - Comparative cytogenetics JID - 101503807 PMC - PMC5646661 OTO - NOTNLM OT - Agropyron OT - FISH OT - P genome OT - rDNA OT - repetitive sequence EDAT- 2017/11/03 06:00 MHDA- 2017/11/03 06:01 PMCR- 2017/08/03 CRDT- 2017/11/03 06:00 PHST- 2017/04/07 00:00 [received] PHST- 2017/06/21 00:00 [accepted] PHST- 2017/11/03 06:00 [entrez] PHST- 2017/11/03 06:00 [pubmed] PHST- 2017/11/03 06:01 [medline] PHST- 2017/08/03 00:00 [pmc-release] AID - 10.3897/CompCytogen.v11i3.13124 [doi] PST - epublish SO - Comp Cytogenet. 2017 Aug 3;11(3):495-509. doi: 10.3897/CompCytogen.v11i3.13124. eCollection 2017.