PMID- 29096159
OWN - NLM
STAT- MEDLINE
DCOM- 20171211
LR  - 20171221
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Linking)
VI  - 513
DP  - 2018 Jan 1
TI  - Porcine reproductive and respiratory disease virus: Evolution and recombination 
      yields distinct ORF5 RFLP 1-7-4 viruses with individual pathogenicity.
PG  - 168-179
LID - S0042-6822(17)30345-8 [pii]
LID - 10.1016/j.virol.2017.10.002 [doi]
AB  - Recent cases of porcine reproductive and respiratory syndrome virus (PRRSV) 
      infection in United States swine-herds have been associated with high mortality 
      in piglets and severe morbidity in sows. Analysis of the ORF5 gene from such 
      clinical cases revealed a unique restriction fragment polymorphism (RFLP) of 
      1-7-4. The genome diversity of seventeen of these viruses (81.4% to 99.8% 
      identical; collected 2013-2015) and the pathogenicity of 4 representative viruses 
      were compared to that of SDSU73, a known moderately virulent strain. 
      Recombination analyses revealed genomic breakpoints in structural and 
      nonstructural regions of the genomes with evidence for recombination events 
      between lineages. Pathogenicity varied between the isolates and the patterns were 
      not consistent. IA/2014/NADC34, IA/2013/ISU-1 and IN/2014/ISU-5 caused more 
      severe disease, and IA/2014/ISU-2 did not cause pyrexia and had little effect on 
      pig growth. ORF5 RFLP genotyping was ineffectual in providing insight into 
      isolate pathogenicity and that other parameters of virulence remain to be 
      identified.
CI  - Published by Elsevier Inc.
FAU - van Geelen, Albert G M
AU  - van Geelen AGM
AD  - Virus and Prion Research Unit, National Animal Disease Center, USDA, Agricultural 
      Research Service, Ames, IA, USA.
FAU - Anderson, Tavis K
AU  - Anderson TK
AD  - Virus and Prion Research Unit, National Animal Disease Center, USDA, Agricultural 
      Research Service, Ames, IA, USA.
FAU - Lager, Kelly M
AU  - Lager KM
AD  - Virus and Prion Research Unit, National Animal Disease Center, USDA, Agricultural 
      Research Service, Ames, IA, USA.
FAU - Das, Phani B
AU  - Das PB
AD  - Virus and Prion Research Unit, National Animal Disease Center, USDA, Agricultural 
      Research Service, Ames, IA, USA.
FAU - Otis, Nicholas J
AU  - Otis NJ
AD  - Virus and Prion Research Unit, National Animal Disease Center, USDA, Agricultural 
      Research Service, Ames, IA, USA.
FAU - Montiel, Nestor A
AU  - Montiel NA
AD  - Virus and Prion Research Unit, National Animal Disease Center, USDA, Agricultural 
      Research Service, Ames, IA, USA.
FAU - Miller, Laura C
AU  - Miller LC
AD  - Virus and Prion Research Unit, National Animal Disease Center, USDA, Agricultural 
      Research Service, Ames, IA, USA.
FAU - Kulshreshtha, Vikas
AU  - Kulshreshtha V
AD  - Virus and Prion Research Unit, National Animal Disease Center, USDA, Agricultural 
      Research Service, Ames, IA, USA.
FAU - Buckley, Alexandra C
AU  - Buckley AC
AD  - Virus and Prion Research Unit, National Animal Disease Center, USDA, Agricultural 
      Research Service, Ames, IA, USA.
FAU - Brockmeier, Susan L
AU  - Brockmeier SL
AD  - Virus and Prion Research Unit, National Animal Disease Center, USDA, Agricultural 
      Research Service, Ames, IA, USA.
FAU - Zhang, Jianqiang
AU  - Zhang J
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA, USA.
FAU - Gauger, Phillip C
AU  - Gauger PC
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA, USA.
FAU - Harmon, Karen M
AU  - Harmon KM
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA, USA.
FAU - Faaberg, Kay S
AU  - Faaberg KS
AD  - Virus and Prion Research Unit, National Animal Disease Center, USDA, Agricultural 
      Research Service, Ames, IA, USA. Electronic address: kay.faaberg@ars.usda.gov.
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20171105
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Animals
MH  - *Evolution, Molecular
MH  - *Genetic Variation
MH  - Genotype
MH  - Polymorphism, Restriction Fragment Length
MH  - Porcine Reproductive and Respiratory Syndrome/epidemiology/pathology/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/*pathogenicity
MH  - *Recombination, Genetic
MH  - Sequence Analysis, DNA
MH  - Swine
MH  - United States/epidemiology
MH  - Viral Envelope Proteins/*genetics
OTO - NOTNLM
OT  - Porcine reproductive and respiratory syndrome virus (PRRSV)
OT  - RFLP1-7-4
OT  - Type 2 genomes
OT  - Viral pathogenesis
OT  - Viral recombination
EDAT- 2017/11/03 06:00
MHDA- 2017/12/12 06:00
CRDT- 2017/11/03 06:00
PHST- 2017/08/15 00:00 [received]
PHST- 2017/09/29 00:00 [revised]
PHST- 2017/10/02 00:00 [accepted]
PHST- 2017/11/03 06:00 [pubmed]
PHST- 2017/12/12 06:00 [medline]
PHST- 2017/11/03 06:00 [entrez]
AID - S0042-6822(17)30345-8 [pii]
AID - 10.1016/j.virol.2017.10.002 [doi]
PST - ppublish
SO  - Virology. 2018 Jan 1;513:168-179. doi: 10.1016/j.virol.2017.10.002. Epub 2017 Nov 
      5.