PMID- 29096559
OWN - NLM
STAT- MEDLINE
DCOM- 20180705
LR  - 20231213
IS  - 1029-2470 (Electronic)
IS  - 1029-2470 (Linking)
VI  - 51
IP  - 11-12
DP  - 2017 Dec
TI  - Retinoic acid receptors alpha and gamma are involved in antioxidative protection in renal 
      tubular epithelial cells injury induced by hypoxia/reoxygenation.
PG  - 873-885
LID - 10.1080/10715762.2017.1387655 [doi]
AB  - Renal interstitial fibrosis (RIF) is a common outcome in various chronic kidney 
      diseases. Injury to renal tubular epithelial cell (RTEC) is major link in RIF. 
      Hypoxia is one of the common factors for RTEC damage. Retinoic acid receptors 
      (RARs), RARalpha, RARbeta and RARgamma, are evolutionary conserved and pleiotropic proteins 
      that have been involved in various cellular functions, including proliferation, 
      differentiation, apoptosis, and transcription. Recently, we discovered that 
      aberrant expression of RARs was involved in the development of RIF in rats. Here, 
      we investigated the role of RARs in the hypoxia/reoxygenation (HR) damage model 
      in RTEC with virus-based delivery vectors to knockdown or overexpress RARs. 
      Relevant indicators were detected. Our results showed that HR inhibited RARalpha and 
      RARgamma expressions in a time-dependent manner in RTECs; however, the expression of 
      RARbeta was not changed obviously. RARalpha and RARgamma overexpression could protect cells 
      from oxidative stress-induced injury by inhibiting HR-induced intracellular 
      superoxide anion (O(2)(-)) generation, cell viability and mitochondria membrane 
      potential (MMP) decrease and transforming growth factor beta1 (TGF-beta1) expression 
      and promoting endogenous antioxidant defense components, superoxide dismutase 
      (SOD) and glutathione (GSH). Meanwhile, inhibition of RARalpha and RARgamma expressions 
      by small interference RNAs (siRNA) resulted in a less resistance of RTEC to HR as 
      shown in increased O(2)(-) production and TGF-beta1 expression and decreased cell 
      viability, MMP, SOD and GSH levels. These data indicates that RARalpha and RARgamma act 
      as positive regulators to offset oxidative damage and profibrosis cytokine 
      accumulation and therefore has an antioxidative effect.
FAU - Jiang, Ling
AU  - Jiang L
AD  - a Department of Pediatrics , The First Affiliated Hospital of Guangxi Medical 
      University , Nanning , PR China.
FAU - Qin, Yuanhan
AU  - Qin Y
AD  - a Department of Pediatrics , The First Affiliated Hospital of Guangxi Medical 
      University , Nanning , PR China.
FAU - Lei, Fengying
AU  - Lei F
AD  - a Department of Pediatrics , The First Affiliated Hospital of Guangxi Medical 
      University , Nanning , PR China.
FAU - Chen, Xiuping
AU  - Chen X
AD  - a Department of Pediatrics , The First Affiliated Hospital of Guangxi Medical 
      University , Nanning , PR China.
FAU - Zhou, Zhiqiang
AU  - Zhou Z
AD  - a Department of Pediatrics , The First Affiliated Hospital of Guangxi Medical 
      University , Nanning , PR China.
LA  - eng
PT  - Journal Article
DEP - 20171103
PL  - England
TA  - Free Radic Res
JT  - Free radical research
JID - 9423872
RN  - 0 (Antioxidants)
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (Retinoic Acid Receptor alpha)
SB  - IM
MH  - Animals
MH  - Antioxidants/*metabolism
MH  - Cell Hypoxia/*physiology
MH  - Epithelial Cells/*metabolism/pathology
MH  - Kidney Diseases/*etiology/metabolism
MH  - Male
MH  - Rats
MH  - Receptors, Retinoic Acid/*metabolism
MH  - Retinoic Acid Receptor alpha/*metabolism
MH  - Retinoic Acid Receptor gamma
OTO - NOTNLM
OT  - Hypoxia/reoxygenation
OT  - oxidative stress
OT  - renal interstitial fibrosis
OT  - renal tubular epithelial cells
OT  - retinoic acid receptors
EDAT- 2017/11/04 06:00
MHDA- 2018/07/06 06:00
CRDT- 2017/11/04 06:00
PHST- 2017/11/04 06:00 [pubmed]
PHST- 2018/07/06 06:00 [medline]
PHST- 2017/11/04 06:00 [entrez]
AID - 10.1080/10715762.2017.1387655 [doi]
PST - ppublish
SO  - Free Radic Res. 2017 Dec;51(11-12):873-885. doi: 10.1080/10715762.2017.1387655. 
      Epub 2017 Nov 3.