PMID- 29098710 OWN - NLM STAT- MEDLINE DCOM- 20191223 LR - 20200930 IS - 1097-4547 (Electronic) IS - 0360-4012 (Linking) VI - 96 IP - 5 DP - 2018 May TI - Physical exercise alters the activation of downstream proteins related to BDNF-TrkB signaling in male Wistar rats with epilepsy. PG - 911-920 LID - 10.1002/jnr.24196 [doi] AB - There are a considerable number of studies concerning the behavioral effects of physical exercise on the epileptic brain; however, the intracellular signaling mechanisms involved remain unclear. We investigated the effects of aerobic exercise on hippocampal levels of brain-derived neurotrophic factor (BDNF), expression of its receptor tropomyosin receptor kinase B (TrkB), and activation of intracellular proteins related to BDNF-TrkB signaling in male Wistar rats with pilocarpine-induced epilepsy. Thirty days after the first spontaneous seizure, rats from the exercise group undertook a 30-day physical exercise program on the treadmill. Thereafter, BDNF levels, expression of TrkB, and activation of intracellular proteins were quantified by enzyme-linked immunosorbent assay, Western blotting, and multiplex assay, respectively. Statistical analyses were conducted using nonparametric tests. Rats with epilepsy presented decreased BDNF levels compared with control rats. BDNF levels increased significantly in the exercise group compared with the epileptic and control groups. Expression of full-length and truncated TrkB was increased in rats with epilepsy, and physical exercise restored its expression to control levels. RAC-alpha serine/threonine-protein kinase, mammalian target of rapamycin, and extracellular signal-regulated kinase activation were reduced in rats with epilepsy, and exercise increased activation compared with control and epilepsy groups. Increased cAMP response element binding protein activation was observed in the exercise group compared with the epilepsy group. Our findings indicate that the beneficial effects of exercise in the epileptic brain can be in part related to alterations in the activation of proteins related to the BDNF-TrkB signaling pathway. CI - (c) 2017 Wiley Periodicals, Inc. FAU - de Almeida, Alexandre Aparecido AU - de Almeida AA AD - Departamento de Fisiologia, Universidade Federal de Sao Paulo, Sao Paulo, Brazil. AD - Instituto Federal Goiano (IF Goiano), Campus Ceres, Ceres, Brazil. FAU - Gomes da Silva, Sergio AU - Gomes da Silva S AD - Hospital Israelita Albert Einstein, Sao Paulo, Brazil. AD - Universidade de Mogi das Cruzes, Mogi das Cruzes, Brazil. FAU - Lopim, Glauber Menezes AU - Lopim GM AD - Departamento de Fisiologia, Universidade Federal de Sao Paulo, Sao Paulo, Brazil. FAU - Vannucci Campos, Diego AU - Vannucci Campos D AD - Departamento de Fisiologia, Universidade Federal de Sao Paulo, Sao Paulo, Brazil. FAU - Fernandes, Jansen AU - Fernandes J AD - Departamento de Fisiologia, Universidade Federal de Sao Paulo, Sao Paulo, Brazil. FAU - Cabral, Francisco Romero AU - Cabral FR AD - Hospital Israelita Albert Einstein, Sao Paulo, Brazil. AD - Faculdade de Ciencias Medicas da Santa Casa de Sao Paulo, Sao Paulo, Brazil. FAU - Arida, Ricardo Mario AU - Arida RM AUID- ORCID: 0000-0002-7771-6133 AD - Departamento de Fisiologia, Universidade Federal de Sao Paulo, Sao Paulo, Brazil. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20171102 PL - United States TA - J Neurosci Res JT - Journal of neuroscience research JID - 7600111 RN - 0 (Bdnf protein, rat) RN - 0 (Brain-Derived Neurotrophic Factor) RN - EC 2.7.10.1 (Ntrk2 protein, rat) RN - EC 2.7.10.1 (Receptor, trkB) SB - IM MH - Animals MH - Brain-Derived Neurotrophic Factor/*metabolism MH - Epilepsy/chemically induced/*metabolism MH - Hippocampus/metabolism MH - Male MH - Physical Conditioning, Animal/*physiology MH - Rats MH - Rats, Wistar MH - Receptor, trkB/*metabolism MH - Seizures/metabolism MH - Signal Transduction OTO - NOTNLM OT - BDNF OT - cell signaling OT - epilepsy OT - physical exercise EDAT- 2017/11/04 06:00 MHDA- 2019/12/24 06:00 CRDT- 2017/11/04 06:00 PHST- 2017/06/28 00:00 [received] PHST- 2017/10/16 00:00 [revised] PHST- 2017/10/17 00:00 [accepted] PHST- 2017/11/04 06:00 [pubmed] PHST- 2019/12/24 06:00 [medline] PHST- 2017/11/04 06:00 [entrez] AID - 10.1002/jnr.24196 [doi] PST - ppublish SO - J Neurosci Res. 2018 May;96(5):911-920. doi: 10.1002/jnr.24196. Epub 2017 Nov 2.