PMID- 29102108
OWN - NLM
STAT- MEDLINE
DCOM- 20180702
LR  - 20180702
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 211
DP  - 2017 Nov
TI  - Increased virulence of a PB2/HA mutant of an avian H9N2 influenza strain after 
      three passages in porcine differentiated airway epithelial cells.
PG  - 129-134
LID - S0378-1135(17)30937-9 [pii]
LID - 10.1016/j.vetmic.2017.10.015 [doi]
AB  - We analyzed the adaptation of influenza viruses to growth in differentiated 
      airway epithelial cells of a new host by passaging an avian H9N2 virus three 
      times in porcine precision-cut lung slices (PCLS). Sequence analysis revealed 
      four mutations: one each in the PB2 and NS1 proteins, and two in the HA protein. 
      In this study, we characterized the PB2 mutation G685R by generating recombinant 
      H9N2 viruses containing the PB2 single mutation alone or in combination with one 
      of the HA mutations (A190V or T212I). When analyzed in porcine cells - a tracheal 
      cell line (NPTr) or PCLS - the PB2-685 mutant did not provide a growth advantage 
      and had no effect on the ciliary activity which is a virulence marker of swine 
      influenza viruses. Pathogenicity for mice was also not increased by the single 
      PB2 mutation. However, both double mutants (HA-190+PB2-685 and HA-212+PB2-685) 
      showed significantly increased virulence in mice. Therefore, the mutations in the 
      HA and PB2 proteins may confer early adaptation of an avian H9N2 virus to a 
      mammalian host. In conclusion, we expect that a broader ensemble of mutations 
      will be required to render an H9N2 virus virulent for pigs.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Yang, Wei
AU  - Yang W
AD  - Institute of Virology, University of Veterinary Medicine Hannover, Hannover, 
      Germany.
FAU - Lambertz, Ruth L O
AU  - Lambertz RLO
AD  - Department of Infection Genetics, Helmholtz Centre for Infection Research and 
      University of Veterinary Medicine Hannover, Braunschweig, Germany.
FAU - Punyadarsaniya, Darsaniya
AU  - Punyadarsaniya D
AD  - Virology and Immunology Department, Faculty of Veterinary Medicine, Mahanakorn 
      University of Technology, Bangkok, Thailand.
FAU - Leist, Sarah R
AU  - Leist SR
AD  - Department of Infection Genetics, Helmholtz Centre for Infection Research and 
      University of Veterinary Medicine Hannover, Braunschweig, Germany.
FAU - Stech, Jurgen
AU  - Stech J
AD  - Institute for Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, 
      Greifswald-Insel Riems, Germany.
FAU - Schughart, Klaus
AU  - Schughart K
AD  - Department of Infection Genetics, Helmholtz Centre for Infection Research and 
      University of Veterinary Medicine Hannover, Braunschweig, Germany; University of 
      Tennessee Health Science Center, Department of Microbiology, Immunology and 
      Biochemistry, Memphis, TN, USA.
FAU - Herrler, Georg
AU  - Herrler G
AD  - Institute of Virology, University of Veterinary Medicine Hannover, Hannover, 
      Germany.
FAU - Wu, Nai-Huei
AU  - Wu NH
AD  - Institute of Virology, University of Veterinary Medicine Hannover, Hannover, 
      Germany. Electronic address: Nai-Huei.Wu@tiho-hannover.de.
FAU - Meng, Fandan
AU  - Meng F
AD  - Institute of Virology, University of Veterinary Medicine Hannover, Hannover, 
      Germany. Electronic address: Fandan.Meng@tiho-hannover.de.
LA  - eng
PT  - Journal Article
DEP - 20171016
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
SB  - IM
MH  - Animals
MH  - Birds
MH  - Cell Line
MH  - Epithelial Cells/virology
MH  - Influenza A Virus, H9N2 Subtype/genetics/*pathogenicity
MH  - Influenza in Birds/*virology
MH  - Lung/virology
MH  - Mice
MH  - Mutation
MH  - Orthomyxoviridae Infections/*veterinary/virology
MH  - Recombination, Genetic
MH  - Respiratory System/virology
MH  - Swine
MH  - Virulence
OTO - NOTNLM
OT  - Adaptation
OT  - Avian influenza viruses
OT  - H9N2
OT  - Precision-cut lung slices
OT  - Well-differentiated epithelial cells
EDAT- 2017/11/06 06:00
MHDA- 2018/07/03 06:00
CRDT- 2017/11/06 06:00
PHST- 2017/08/08 00:00 [received]
PHST- 2017/10/09 00:00 [revised]
PHST- 2017/10/12 00:00 [accepted]
PHST- 2017/11/06 06:00 [entrez]
PHST- 2017/11/06 06:00 [pubmed]
PHST- 2018/07/03 06:00 [medline]
AID - S0378-1135(17)30937-9 [pii]
AID - 10.1016/j.vetmic.2017.10.015 [doi]
PST - ppublish
SO  - Vet Microbiol. 2017 Nov;211:129-134. doi: 10.1016/j.vetmic.2017.10.015. Epub 2017 
      Oct 16.