PMID- 29103678
OWN - NLM
STAT- MEDLINE
DCOM- 20180626
LR  - 20180626
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 210
DP  - 2017 Oct
TI  - Cytotoxic activity of Flavobacterium psychrophilum in skeletal muscle cells of 
      rainbow trout (Oncorhynchus mykiss).
PG  - 101-106
LID - S0378-1135(17)30123-2 [pii]
LID - 10.1016/j.vetmic.2017.09.009 [doi]
AB  - Flavobacterium psychrophilum is the etiologic agent of bacterial coldwater 
      disease (BCWD) and rainbow trout fry syndrome (RTFS), which cause significant 
      worldwide losses in aquaculture. Juvenile rainbow trout are particularly 
      susceptible to F. psychrophilum infection, the main external clinical signs of 
      which are extensive necrotic myositis and ulcerative lesions. Despite the 
      economic relevance of this pathogen in aquaculture, little is known about the 
      molecular mechanisms underlying F. psychrophilum infection and pathogenesis. In 
      this study, cultured skeletal muscle cells from rainbow trout (Oncorhynchus 
      mykiss) were co-incubated with the virulent strain of F. psychrophilum JIP02/86 
      (ATCC 49511). Trypan blue exclusion analysis at 48h post-incubation revealed 
      decreased cellular viability. Direct bacteria-myoblast contact was found a key 
      factor in inducing F. psychrophilum cytotoxicity. Apoptosis was characterized by 
      nuclear DNA fragmentation, decreased plasma membrane integrity, increased caspase 
      activity, and the proteolytic cleavage of poly(ADP-ribose)polymerase-1 (PARP-1). 
      Moreover, bacterial infection induced an early inhibition of NF-kappaB signaling, as 
      well as a differential expression of the pro- and anti-apoptotic genes, bax and 
      bcl-2. These findings suggest that F. psychrophilum induces rainbow trout muscle 
      apoptosis through the modulation of the NF-kappaB signaling as a mechanism for 
      nutrient acquisition and survival.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Iturriaga, Mathias
AU  - Iturriaga M
AD  - Universidad Andres Bello, Departamento Ciencias Biologicas, Facultad Ciencias 
      Biologicas, 8370146 Santiago, Chile.
FAU - Espinoza, Marlen Brisa
AU  - Espinoza MB
AD  - Universidad Andres Bello, Departamento Ciencias Biologicas, Facultad Ciencias 
      Biologicas, 8370146 Santiago, Chile.
FAU - Poblete-Morales, Matias
AU  - Poblete-Morales M
AD  - Universidad Andres Bello, Departamento Ciencias Biologicas, Facultad Ciencias 
      Biologicas, 8370146 Santiago, Chile; Interdisciplinary Center for Aquaculture 
      Research (INCAR), 4030000 Concepcion, Chile.
FAU - Feijoo, Carmen Gloria
AU  - Feijoo CG
AD  - Universidad Andres Bello, Departamento Ciencias Biologicas, Facultad Ciencias 
      Biologicas, 8370146 Santiago, Chile; Interdisciplinary Center for Aquaculture 
      Research (INCAR), 4030000 Concepcion, Chile.
FAU - Reyes, Ariel E
AU  - Reyes AE
AD  - Universidad Andres Bello, Departamento Ciencias Biologicas, Facultad Ciencias 
      Biologicas, 8370146 Santiago, Chile; Interdisciplinary Center for Aquaculture 
      Research (INCAR), 4030000 Concepcion, Chile.
FAU - Molina, Alfredo
AU  - Molina A
AD  - Universidad Andres Bello, Departamento Ciencias Biologicas, Facultad Ciencias 
      Biologicas, 8370146 Santiago, Chile; Interdisciplinary Center for Aquaculture 
      Research (INCAR), 4030000 Concepcion, Chile; Universidad Andres Bello, Centro de 
      Investigacion Marina Quintay (CIMARQ), Facultad de Ecologia y Recursos Naturales, 
      2340000 Valparaiso, Chile.
FAU - Avendano-Herrera, Ruben
AU  - Avendano-Herrera R
AD  - Universidad Andres Bello, Departamento Ciencias Biologicas, Facultad Ciencias 
      Biologicas, 8370146 Santiago, Chile; Interdisciplinary Center for Aquaculture 
      Research (INCAR), 4030000 Concepcion, Chile; Universidad Andres Bello, Centro de 
      Investigacion Marina Quintay (CIMARQ), Facultad de Ecologia y Recursos Naturales, 
      2340000 Valparaiso, Chile. Electronic address: ravendano@unab.cl.
FAU - Valdes, Juan Antonio
AU  - Valdes JA
AD  - Universidad Andres Bello, Departamento Ciencias Biologicas, Facultad Ciencias 
      Biologicas, 8370146 Santiago, Chile; Interdisciplinary Center for Aquaculture 
      Research (INCAR), 4030000 Concepcion, Chile; Universidad Andres Bello, Centro de 
      Investigacion Marina Quintay (CIMARQ), Facultad de Ecologia y Recursos Naturales, 
      2340000 Valparaiso, Chile. Electronic address: jvaldes@unab.cl.
LA  - eng
PT  - Journal Article
DEP - 20170918
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Aquaculture
MH  - Cell Survival
MH  - Fish Diseases/*microbiology
MH  - Flavobacteriaceae Infections/microbiology/*veterinary
MH  - Flavobacterium/*physiology
MH  - Muscle, Skeletal/microbiology
MH  - Myoblasts/microbiology
MH  - Oncorhynchus mykiss/*microbiology
OTO - NOTNLM
OT  - Apoptosis
OT  - Flavobacterium psychrophilum
OT  - Rainbow trout
OT  - Skeletal muscle
EDAT- 2017/11/07 06:00
MHDA- 2018/06/27 06:00
CRDT- 2017/11/07 06:00
PHST- 2017/01/29 00:00 [received]
PHST- 2017/09/15 00:00 [revised]
PHST- 2017/09/16 00:00 [accepted]
PHST- 2017/11/07 06:00 [entrez]
PHST- 2017/11/07 06:00 [pubmed]
PHST- 2018/06/27 06:00 [medline]
AID - S0378-1135(17)30123-2 [pii]
AID - 10.1016/j.vetmic.2017.09.009 [doi]
PST - ppublish
SO  - Vet Microbiol. 2017 Oct;210:101-106. doi: 10.1016/j.vetmic.2017.09.009. Epub 2017 
      Sep 18.