PMID- 29103681
OWN - NLM
STAT- MEDLINE
DCOM- 20180626
LR  - 20220330
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 210
DP  - 2017 Oct
TI  - Phenotypic characterization of a highly pathogenic Italian porcine reproductive 
      and respiratory syndrome virus (PRRSV) type 1 subtype 1 isolate in experimentally 
      infected pigs.
PG  - 124-133
LID - S0378-1135(17)30892-1 [pii]
LID - 10.1016/j.vetmic.2017.09.002 [doi]
AB  - Highly pathogenic (HP) isolates of the PRRS virus started emerging in North 
      America and Asia in the late 1990s. More recently, they have emerged in Europe. 
      These isolates are characterized by high viral loads, severe general clinical 
      signs and high mortality, in sows, weaners and growers. Their genome shows a 
      discontinuous aminoacids deletion in the non-structural protein 2 (NSP2). The 
      present study was aimed at characterizing the clinical, pathological and 
      immunological features of a highly pathogenetic, Italian PRRSV-1 subtype 1 
      isolate (PRRSV1_PR40/2014), following experimental infection in conventional 
      4-weeks-old pigs. The PRRSV1_PR40/2014 infected group showed severe clinical 
      signs (high fever and dispnoea). Pathological lesions, including severe 
      lymphocytopenia in bronchial lymph-nodes and thymus were also recorded. Higher 
      serum PRRSV genome copies and lower virus neutralizing antibody titer were 
      observed in the PR40 group, when compared to the group infected with a 
      conventional PRRSV strain. The genetic analysis of the strain, and the phenotypic 
      features observed in the field and reproduced in the experimental study, 
      confirmed the high pathogenicity of the Italian PRRSV-1 subtype 1 PR40 isolate.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Canelli, Elena
AU  - Canelli E
AD  - Department of Veterinary Science, University of Parma, Strada del Taglio 10, 
      43126, Parma, Italy. Electronic address: elena.canelli@unipr.it.
FAU - Catella, Alessia
AU  - Catella A
AD  - Department of Veterinary Science, University of Parma, Strada del Taglio 10, 
      43126, Parma, Italy.
FAU - Borghetti, Paolo
AU  - Borghetti P
AD  - Department of Veterinary Science, University of Parma, Strada del Taglio 10, 
      43126, Parma, Italy.
FAU - Ferrari, Luca
AU  - Ferrari L
AD  - Department of Veterinary Science, University of Parma, Strada del Taglio 10, 
      43126, Parma, Italy.
FAU - Ogno, Giulia
AU  - Ogno G
AD  - Department of Veterinary Science, University of Parma, Strada del Taglio 10, 
      43126, Parma, Italy.
FAU - De Angelis, Elena
AU  - De Angelis E
AD  - Department of Veterinary Science, University of Parma, Strada del Taglio 10, 
      43126, Parma, Italy.
FAU - Corradi, Attilio
AU  - Corradi A
AD  - Department of Veterinary Science, University of Parma, Strada del Taglio 10, 
      43126, Parma, Italy.
FAU - Passeri, Benedetta
AU  - Passeri B
AD  - Department of Veterinary Science, University of Parma, Strada del Taglio 10, 
      43126, Parma, Italy.
FAU - Bertani, Valeria
AU  - Bertani V
AD  - Department of Veterinary Science, University of Parma, Strada del Taglio 10, 
      43126, Parma, Italy.
FAU - Sandri, Giampietro
AU  - Sandri G
AD  - Gruppo Veronesi, Via Valpantena 18/G, 37142, Verona, Italy.
FAU - Bonilauri, Paolo
AU  - Bonilauri P
AD  - IZSLER, Istituto Zooprofilattico Sperimentale Della Lombardia e dell'Emilia 
      Romagna, Unit of Reggio Emilia, Via Pitagora 2, 42100, Reggio Emilia, Italy.
FAU - Leung, Frederick C
AU  - Leung FC
AD  - School of Biological Science, The University of Hong Kong, Hong Kong, China; FCL 
      Bioscience, Hong Kong Science and Technology Park, Shatin, New Territories, Hong 
      Kong, China.
FAU - Guazzetti, Stefano
AU  - Guazzetti S
AD  - AUSL Reggio Emilia, Via Giovanni Amendola 2, 42122, Reggio Emilia, Italy.
FAU - Martelli, Paolo
AU  - Martelli P
AD  - Department of Veterinary Science, University of Parma, Strada del Taglio 10, 
      43126, Parma, Italy.
LA  - eng
PT  - Journal Article
DEP - 20170915
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (RNA, Viral)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
RN  - EC 3.4.22.- (nsP2 proteinase)
SB  - IM
MH  - Animals
MH  - Antibodies, Neutralizing/blood
MH  - Cysteine Endopeptidases/*genetics
MH  - Lymphopenia/pathology/*veterinary/virology
MH  - Phenotype
MH  - Phylogeny
MH  - Porcine Reproductive and Respiratory Syndrome/pathology/*virology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*pathogenicity
MH  - RNA, Viral/blood
MH  - Sequence Deletion
MH  - Swine
MH  - Viral Load
MH  - Viremia/veterinary
MH  - Virulence
MH  - Weaning
OTO - NOTNLM
OT  - Clinical signs
OT  - HP-PRRSV
OT  - Highly pathogenic
OT  - Immunity
OT  - PRRSV-1
OT  - Viremia
EDAT- 2017/11/07 06:00
MHDA- 2018/06/27 06:00
CRDT- 2017/11/07 06:00
PHST- 2017/07/24 00:00 [received]
PHST- 2017/09/01 00:00 [revised]
PHST- 2017/09/06 00:00 [accepted]
PHST- 2017/11/07 06:00 [entrez]
PHST- 2017/11/07 06:00 [pubmed]
PHST- 2018/06/27 06:00 [medline]
AID - S0378-1135(17)30892-1 [pii]
AID - 10.1016/j.vetmic.2017.09.002 [doi]
PST - ppublish
SO  - Vet Microbiol. 2017 Oct;210:124-133. doi: 10.1016/j.vetmic.2017.09.002. Epub 2017 
      Sep 15.