PMID- 29103907
OWN - NLM
STAT- MEDLINE
DCOM- 20190415
LR  - 20210225
IS  - 1871-403X (Print)
IS  - 1871-403X (Linking)
VI  - 12
IP  - 2
DP  - 2018 Mar-Apr
TI  - Myeloid ATG16L1 does not affect adipose tissue inflammation or body mass in mice 
      fed high fat diet.
PG  - 174-186
LID - S1871-403X(17)30118-7 [pii]
LID - 10.1016/j.orcp.2017.10.006 [doi]
AB  - BACKGROUND: An influx of lipid-loaded macrophages characterizes visceral adipose 
      tissue (VAT) inflammation, which is an important factor in the development of 
      insulin resistance (IR) in obesity. Depletion of macrophage lipids accompanies 
      increased whole body insulin sensitivity, but the underlying mechanism is 
      unknown. Deficiency of autophagy protein ATG16L1 is associated with increases in 
      inflammatory diseases and lipid metabolism, but the connection between ATG16L1, 
      IR, and obesity remains elusive. We hypothesize that myeloid ATG16L1 contributes 
      to lipid loading in macrophages and to IR. METHODS: Wild-type (WT) bone marrow 
      derived macrophages (BMDMs) were treated with fatty acids and assessed for 
      markers of autophagy. Myeloid-deficient Atg16l1 and littermate control male mice 
      were fed high fat diet (HFD) or low fat diet (LFD) for 3 months starting at 8 
      weeks of age. Mice were assessed for body mass, fat and lean mass, glucose and 
      insulin sensitivity, food consumption and adipose inflammation. 
      Fluorescence-activated cell sorted VAT macrophages were assessed for lipid 
      content and expression of autophagy related genes. RESULTS: VAT and VAT 
      macrophages from HFD-fed WT mice did not show differences in autophagy protein 
      and gene expression compared to tissue from LFD-fed mice. Fatty acid-treated 
      BMDMs increased neutral lipid content but did not change autophagy protein 
      expression. HFD-fed Atg16l1 myeloid-deficient and littermate mice demonstrated no 
      differences in body mass, glucose or insulin sensitivity, food consumption, fat 
      or lean mass, macrophage lipid content, or adipose tissue inflammation. 
      CONCLUSION: ATG16L1 does not contribute to obesity, IR, adipose tissue 
      inflammation or lipid loading in macrophages in mice fed HFD.
CI  - Copyright (c) 2017 Asia Oceania Association for the Study of Obesity. Published by 
      Elsevier Ltd. All rights reserved.
FAU - Litwinoff, Evelyn M S
AU  - Litwinoff EMS
AD  - Diabetes Research Program, Division of Endocrinology, Diabetes and Metabolism, 
      Department of Medicine, NYU Langone Health, New York, NY 10016, USA.
FAU - Gold, Merav Y
AU  - Gold MY
AD  - Diabetes Research Program, Division of Endocrinology, Diabetes and Metabolism, 
      Department of Medicine, NYU Langone Health, New York, NY 10016, USA.
FAU - Singh, Karan
AU  - Singh K
AD  - Diabetes Research Program, Division of Endocrinology, Diabetes and Metabolism, 
      Department of Medicine, NYU Langone Health, New York, NY 10016, USA.
FAU - Hu, Jiyuan
AU  - Hu J
AD  - Departments of Population Health (Biostatistics) and Environmental Medicine, NYU 
      Langone Health, New York, NY 10016, USA.
FAU - Li, Huilin
AU  - Li H
AD  - Departments of Population Health (Biostatistics) and Environmental Medicine, NYU 
      Langone Health, New York, NY 10016, USA.
FAU - Cadwell, Ken
AU  - Cadwell K
AD  - Kimmel Center for Biology and Medicine at the Skirball Institute, and the 
      Department of Microbiology, NYU Langone Health, New York, NY 10016, USA.
FAU - Schmidt, Ann Marie
AU  - Schmidt AM
AD  - Kimmel Center for Biology and Medicine at the Skirball Institute, and the 
      Department of Microbiology, NYU Langone Health, New York, NY 10016, USA. 
      Electronic address: Annmarie.Schmidt@nyumc.org.
LA  - eng
GR  - P01 HL131481/HL/NHLBI NIH HHS/United States
GR  - P30 CA016087/CA/NCI NIH HHS/United States
GR  - R01 DK109675/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20171102
PL  - Netherlands
TA  - Obes Res Clin Pract
JT  - Obesity research & clinical practice
JID - 101303911
RN  - 0 (Atg16l1 protein, mouse)
RN  - 0 (Autophagy-Related Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Fatty Acids)
SB  - IM
MH  - Adipose Tissue/*pathology
MH  - Animals
MH  - Autophagy-Related Proteins
MH  - Carrier Proteins/*physiology
MH  - *Diet, High-Fat
MH  - Disease Models, Animal
MH  - Energy Intake
MH  - Fatty Acids
MH  - Gene Expression Regulation
MH  - Inflammation/*genetics/*pathology
MH  - Lipid Metabolism/genetics
MH  - Macrophages/*physiology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Obesity/genetics/pathology
PMC - PMC5932285
MID - NIHMS917225
OTO - NOTNLM
OT  - Adipose tissue
OT  - Autophagy
OT  - Inflammation
OT  - Obesity
EDAT- 2017/11/07 06:00
MHDA- 2019/04/16 06:00
PMCR- 2019/03/01
CRDT- 2017/11/07 06:00
PHST- 2017/05/26 00:00 [received]
PHST- 2017/10/04 00:00 [revised]
PHST- 2017/10/17 00:00 [accepted]
PHST- 2017/11/07 06:00 [pubmed]
PHST- 2019/04/16 06:00 [medline]
PHST- 2017/11/07 06:00 [entrez]
PHST- 2019/03/01 00:00 [pmc-release]
AID - S1871-403X(17)30118-7 [pii]
AID - 10.1016/j.orcp.2017.10.006 [doi]
PST - ppublish
SO  - Obes Res Clin Pract. 2018 Mar-Apr;12(2):174-186. doi: 10.1016/j.orcp.2017.10.006. 
      Epub 2017 Nov 2.