PMID- 29104466
OWN - NLM
STAT- MEDLINE
DCOM- 20180625
LR  - 20181202
IS  - 1449-1907 (Electronic)
IS  - 1449-1907 (Linking)
VI  - 14
IP  - 11
DP  - 2017
TI  - The Crosstalk between HDPSCs and HUCMSCs on Proliferation and Osteogenic Genes 
      Expression in Coculture System.
PG  - 1118-1129
LID - 10.7150/ijms.19814 [doi]
AB  - Objectives: The present study established a non-contact coculture system in 
      vitro, aiming to investigate the crosstalk between human dental pulp stem cells 
      (hDPSCs) and human umbilical cord mesenchymal stem cells (hUCMSCs) on 
      proliferation activity and osteogenic genes expression through paracrine. 
      Materials and methods: The stemness of hDPSCs and hUCMSCs were identified by flow 
      cytometric analysis and multipotential differentiation assays. With the help of 
      transwell inserts, the non-contact coculture system in vitro was established 
      between hDPSCs and hUCMSCs. EdU labeling analysis and Western Blot were used to 
      detect the proliferation activity. The mRNA and protein levels of osteogenic 
      genes were evaluated by RT-PCR and Western Blot. The expression of elements in 
      Akt/mTOR signaling pathway were detected by Western Blot. Results: Both hDPSCs 
      and hUCMSCs were positive to MSCs specific surface markers and had 
      multi-differentiation potential. The proportion of EdU-positive cells increased 
      and the expression of CDK6 and CYCLIN A were up-regulated in cocultured hDPSCs. 
      Both prior coculture and persistent coculture improved mRNA and protein levels of 
      osteogenic genes in hDPSCs. While in cocultured hUCMSCs, no statistical 
      differences were observed on proliferation and osteogenesis. The phosphorylation 
      of Akt and mTOR was up-regulated in cocultured hDPSCs. Conclusions: The crosstalk 
      between hDPSCs and hUCMSCs in coculture system increased the proliferation 
      activity and enhanced osteogenic genes expression in hDPSCs. Akt/mTOR signaling 
      pathway might take part in the enhancing effects in both cell proliferation and 
      gene expression.
FAU - Jia, Linglu
AU  - Jia L
AD  - School of Stomatology, Shandong University, Jinan, China.
AD  - Shandong provincial key laboratory of oral tissue regeneration, Jinan, China.
FAU - Gu, Weiting
AU  - Gu W
AD  - Qilu hospital of Shandong University, Jinan, China.
FAU - Zhang, Yunpeng
AU  - Zhang Y
AD  - School of Stomatology, Shandong University, Jinan, China.
AD  - Shandong provincial key laboratory of oral tissue regeneration, Jinan, China.
FAU - Ji, Yawen
AU  - Ji Y
AD  - School of Stomatology, Shandong University, Jinan, China.
AD  - Shandong provincial key laboratory of oral tissue regeneration, Jinan, China.
FAU - Liang, Jin
AU  - Liang J
AD  - School of Stomatology, Shandong University, Jinan, China.
AD  - Shandong provincial key laboratory of oral tissue regeneration, Jinan, China.
FAU - Wen, Yong
AU  - Wen Y
AD  - School of Stomatology, Shandong University, Jinan, China.
AD  - Shandong provincial key laboratory of oral tissue regeneration, Jinan, China.
FAU - Xu, Xin
AU  - Xu X
AD  - School of Stomatology, Shandong University, Jinan, China.
AD  - Shandong provincial key laboratory of oral tissue regeneration, Jinan, China.
LA  - eng
PT  - Journal Article
DEP - 20170904
PL  - Australia
TA  - Int J Med Sci
JT  - International journal of medical sciences
JID - 101213954
SB  - IM
MH  - Blotting, Western
MH  - Cell Differentiation/physiology
MH  - Cell Proliferation/physiology
MH  - Coculture Techniques/*methods
MH  - Dental Pulp/cytology/metabolism
MH  - Flow Cytometry
MH  - Humans
MH  - Mesenchymal Stem Cells/cytology/metabolism
MH  - Osteogenesis/drug effects
PMC - PMC5666543
OTO - NOTNLM
OT  - crosstalk.
OT  - human dental pulp stem cells
OT  - human umbilical cord mesenchymal stem cells
OT  - osteogenesis
OT  - proliferation
COIS- Competing Interests: The authors have declared that no competing interest exists.
EDAT- 2017/11/07 06:00
MHDA- 2018/06/26 06:00
PMCR- 2017/01/01
CRDT- 2017/11/07 06:00
PHST- 2017/02/26 00:00 [received]
PHST- 2017/06/19 00:00 [accepted]
PHST- 2017/11/07 06:00 [entrez]
PHST- 2017/11/07 06:00 [pubmed]
PHST- 2018/06/26 06:00 [medline]
PHST- 2017/01/01 00:00 [pmc-release]
AID - ijmsv14p1118 [pii]
AID - 10.7150/ijms.19814 [doi]
PST - epublish
SO  - Int J Med Sci. 2017 Sep 4;14(11):1118-1129. doi: 10.7150/ijms.19814. eCollection 
      2017.