PMID- 29104633
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 14
IP  - 5
DP  - 2017 Nov
TI  - Echinocystic acid ameliorates hyperhomocysteinemia-induced vascular endothelial 
      cell injury through regulating NF-kappaB and CYP1A1.
PG  - 4174-4180
LID - 10.3892/etm.2017.5097 [doi]
AB  - The present study investigated the role of echinocystic acid (EA) on the 
      expression of nuclear factor (NF)-kappaB and cytochrome P450 1A1 (CYP1A1), and aortic 
      morphology, in a rat model of hyperhomocysteinemia (Hhcy). A total of 50 Sprague 
      Dawley rats were randomly divided into five groups as follows: Normal control 
      (NC), model control (MC), vitamin control (VC; folic acid 1 mg/kg + vitamin B2 2 
      mg/kg + vitamin B12 10u g/kg), EA1 (20 mg/kg EA) and EA2 (40 mg/kg EA). Plasma 
      homocysteine (Hcy) levels were determined via high performance liquid 
      chromatography, and the morphology of the aorta was investigated using 
      hematoxylin and eosin staining. Furthermore, aortic mRNA and protein levels of 
      NF-kappaB and CYP1A1 were measured using reverse transcription-quantitative 
      polymerase chain reaction analysis and western blotting, respectively. Plasma Hcy 
      levels, and aortic mRNA and protein levels of NF-kappaB and CYP1A1, were 
      significantly lower in the EA-treated group compared with the MC group (all 
      P<0.05). However, the aortic morphology remained normal, including the 
      endothelial cells of the inner layer, and smooth muscle cells of the media layer 
      and adventitia. In conclusion, the results of the present study indicate that EA 
      has a protective role on vascular endothelial cells in Hhcy through decreasing 
      plasma Hcy, and thus NF-kappaB and CYP1A1 expression.
FAU - Huang, Chuan-Feng
AU  - Huang CF
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      Wuhan University, Wuhan, Hubei 430071, P.R. China.
AD  - Department of Pharmacology, Basic Medical School, Nanyang Medical College, 
      Nanyang, Henan 473003, P.R. China.
FAU - Wang, Wei-Na
AU  - Wang WN
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      Wuhan University, Wuhan, Hubei 430071, P.R. China.
AD  - Department of Pharmacology, Basic Medical School, Nanyang Medical College, 
      Nanyang, Henan 473003, P.R. China.
FAU - Sun, Cheng-Cao
AU  - Sun CC
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      Wuhan University, Wuhan, Hubei 430071, P.R. China.
FAU - Wang, Yu-Qing
AU  - Wang YQ
AD  - Department of Pharmacology, Basic Medical School, Nanyang Medical College, 
      Nanyang, Henan 473003, P.R. China.
FAU - Li, Ling
AU  - Li L
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      Wuhan University, Wuhan, Hubei 430071, P.R. China.
AD  - Department of Pharmacology, Basic Medical School, Nanyang Medical College, 
      Nanyang, Henan 473003, P.R. China.
FAU - Li, Yin
AU  - Li Y
AD  - Department of Pharmacology, Basic Medical School, Nanyang Medical College, 
      Nanyang, Henan 473003, P.R. China.
FAU - Li, De-Jia
AU  - Li DJ
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      Wuhan University, Wuhan, Hubei 430071, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20170901
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC5658691
OTO - NOTNLM
OT  - cytochrome P450 1A1
OT  - echinocystic acid
OT  - hyperhomocysteinemia
OT  - nuclear factor kappaB
OT  - vascular endothelial cells
EDAT- 2017/11/07 06:00
MHDA- 2017/11/07 06:01
PMCR- 2017/09/01
CRDT- 2017/11/07 06:00
PHST- 2016/04/13 00:00 [received]
PHST- 2017/04/28 00:00 [accepted]
PHST- 2017/11/07 06:00 [entrez]
PHST- 2017/11/07 06:00 [pubmed]
PHST- 2017/11/07 06:01 [medline]
PHST- 2017/09/01 00:00 [pmc-release]
AID - ETM-0-0-5097 [pii]
AID - 10.3892/etm.2017.5097 [doi]
PST - ppublish
SO  - Exp Ther Med. 2017 Nov;14(5):4174-4180. doi: 10.3892/etm.2017.5097. Epub 2017 Sep 
      1.