PMID- 29105463
OWN - NLM
STAT- MEDLINE
DCOM- 20190403
LR  - 20220410
IS  - 1000-0607 (Print)
IS  - 1000-0607 (Linking)
VI  - 42
IP  - 5
DP  - 2017 Oct 25
TI  - [Moxibustion Improves Learning Ability by Regulating Hippocampal Neurotrophic 
      Factor Expression and Notch Signaling in Vascular Dementia Rats].
PG  - 377-83
AB  - OBJECTIVE: To observe the effect of moxibustion on the learning ability and 
      expression of neurotrophic factors and Notch signaling in vascular dementia (VD) 
      rats, so as to explore its neurogenesis mechanism underlying improvement of VD. 
      METHODS: Sixty SD rats were equally and randomly divided into sham operation 
      (sham), model, medication and moxibustion groups (n=15 rats/group). The VD model 
      was established by occlusion of the bilateral cervical common arteries and 
      reperfusion. Moxibustion was applied to "Dazhui"(GV 14), "Guanyuan"(CV 4) and 
      "Mingmen"(GV 4)for 15 minutes, once daily, 6 times a week for 4 weeks. Rats of 
      the medication group were treated by gavage of nimodipine (2 mg.kg(-1).d(-1)),3 
      times a day, 6 days a week for 4 weeks. Morris water maze tests were performed to 
      detect the rat's learning-memory ability. The infarcted size of the brain was 
      detected by using 2,3,5-triphenyltetrazolium chloride (TTC) staining, and H.E. 
      staining was used to detect the histopathological changes. The expression level 
      of glia fibrillary acidic protein (GFAP), nerve growth factor (NGF), brain 
      derived neurotrophic factor (BDNF), Notch 1 (a receptor), Hes 3 (a downstream 
      effector) mRNAs and proteins in the hippocampal tissues were detected by 
      quantitative real-time PCR and Western blot, respectively. RESULTS: After 4 
      weeks' intervention, modeling-induced increase of escape latency was 
      significantly shortened in both moxibustion and medication groups relevant to the 
      model group (P<0.05), and the infarct size was reduced and the damage degree of 
      nerve cells in the brain tissue alleviated. The expression levels of BDNF, NGF, 
      GFAP, Hes 3, Notch 1 genes and proteins were significantly up-regulated in the 
      model group relevant to the sham operation group (P<0.05,P<0.01). After the 
      intervention, the expression levels of hippocampal BDNF, NGF, GFAP, Hes 3 and 
      Notch 1 mRNAs and proteins in the moxibustion group, and NGF and GFAP mRNAs, and 
      BDNF, NGF, GFAP, Hes 3 and Notch 1 proteins in the medication group were further 
      obviously up-regulated relevant to the model group (P<0.05, P<0.01). The effect 
      of moxibustion was significantly superior to that of medication in up-regulating 
      GFAP, Hes 3, Notch 1 mRNAs expression (P<0.05,P<0.01). No significant differences 
      were found in the expression of BDNF, Hes 3 and Notch 1 mRNAs in the medication 
      group relevant to the model group (P<0.05), and between the moxibustion and 
      medication groups in up-regulating the expression of BDNF and NGF mRNAs, and in 
      up-regulating the expression of BDNF, NGF, GFAP, Hes 3 and Notch 1 proteins 
      (P<0.05). CONCLUSIONS: Moxibustion can improve learning ability in VD rats, which 
      may be associated with its effects in up-regulating the expression of 
      neurotrophic factors and in potentiating Notch signaling.
FAU - Wang, Ying
AU  - Wang Y
AD  - College of Acu-moxibustion and Massage, Anhui University of Traditional Chinese 
      Medicine, Hefei 230061, China.
FAU - Cai, Sheng-Chao
AU  - Cai SC
AD  - Second Affiliated Hospital of Anhui University of Traditional Chinese Medicine, 
      Hefei 230061. caishengchao@sina.com.
FAU - Song, Xiao-Ge
AU  - Song XG
AD  - Institute of Acupuncture-moxibustion and Meridian, Anhui University of 
      Traditional Chinese Medicine, Hefei 230038.
FAU - Zhu, Wan-Li
AU  - Zhu WL
AD  - College of Acu-moxibustion and Massage, Anhui University of Traditional Chinese 
      Medicine, Hefei 230061, China.
FAU - Yang, Kun
AU  - Yang K
AD  - Second Affiliated Hospital of Anhui University of Traditional Chinese Medicine, 
      Hefei 230061.
FAU - Qian, Jian-Jian
AU  - Qian JJ
AD  - College of Acu-moxibustion and Massage, Anhui University of Traditional Chinese 
      Medicine, Hefei 230061, China.
FAU - Ding, Nuo-Nuo
AU  - Ding NN
AD  - College of Acu-moxibustion and Massage, Anhui University of Traditional Chinese 
      Medicine, Hefei 230061, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhen Ci Yan Jiu
JT  - Zhen ci yan jiu = Acupuncture research
JID - 8507710
SB  - IM
MH  - Animals
MH  - *Dementia, Vascular
MH  - Hippocampus
MH  - Learning
MH  - *Moxibustion
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2017/11/07 06:00
MHDA- 2019/04/04 06:00
CRDT- 2017/11/07 06:00
PHST- 2017/11/07 06:00 [entrez]
PHST- 2017/11/07 06:00 [pubmed]
PHST- 2019/04/04 06:00 [medline]
PST - ppublish
SO  - Zhen Ci Yan Jiu. 2017 Oct 25;42(5):377-83.